Structural characteristics are crucial to the benefits of guar gum in experimental osteoarthritis

Castro, Rondinelle Ribeiro; Silva, Christine Maria M.; Nunes, Rodolfo de Melo; Cunha, Pablyana Leila Rodrigues da; Paula, Regina C.M. de; Feitosa, Judith P.A.; Girão-Carmona, Virgínia Cláudia Carneiro; Pompeu, Margarida M. L.; Leite, José Alberto Dias; Rocha, Francisco Airton Castro

doi:10.1016/j.carbpol.2016.05.031

Cited by 20 publications

(17 citation statements)

References 21 publications

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“…Injection of this GM into the knee of rats subjected to experimental OA provided analgesia similar to that of HY regardless of using a viscous or a salinesoluble GM formulation, showing that viscosupplementation benefits may not depend on the viscoelastic properties [13]. We also showed that chronic GM administration prevented joint damage in an OA model and structure modifications of GM abrogate the therapeutic benefit [14].…”

Section: Introductionmentioning

confidence: 60%

Hylan G-F20 and galactomannan joint flares are associated to acute synovitis and release of inflammatory cytokines

et al. 2020

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Background: Injection of Hylan G-F20 (HY) into joints may provoke local flares, which mechanisms may involve reaction to protein contaminants. We have previously developed a protein-free saline-soluble galactomannan derived from guar gum (GM) that displays both analgesia and chondroprotection in experimental osteoarthritis (OA). We now demonstrate that both GM and Hylan G-F20 (HY) promote mild synovitis with cytokine release after intra-articular injection. Methods: Mice received 100 μg/25 μL GM or HY or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, interleukin (IL)-1, IL-6, and CXCL-1 (pg/mL) levels were assessed in joint lavage at 6 h. Synovia were excised for histopathology. Results: Neither GM nor HY after being given into mice knee joints induced pain albeit promoting mild cell influx into joint washings as well as mild synovitis at histology, with no damage to the underlying cartilage. HY but not GM promoted IL-1 release into mice joints. Both compounds induced IL-6 and CXCL-1 release. Conclusion: Intra-articular injection of HY or GM promote acute transient synovitis whilst not provoking detectable significant joint damage. Local administration of these polysaccharides induces acute intra-articular release of inflammatory cytokines, which may account for joint flares following viscosupplementation.

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Section: Introductionmentioning

confidence: 60%

Hylan G-F20 and galactomannan joint flares are associated to acute synovitis and release of inflammatory cytokines

et al. 2020

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“…Moreover, that GG-derived galactomannan analgesia occurred both with a highly viscous and a saline-soluble preparation, suggesting that the effect was independent of the gel state. Recently, the same group showed (5) that modifications of the chemical structure of that galactomannan, namely oxidation or sulfation, abrogated the analgesia provided by the original compound, using the ACLT rat OA model. Additionally, weekly administrations, starting 14 days until 70 days post-ACLT prevented structural joint damage to the knee, as compared to controls.…”

Section: Structural Properties Of the Polysaccharides Used In Vsmentioning

confidence: 99%

“…Additionally, weekly administrations, starting 14 days until 70 days post-ACLT prevented structural joint damage to the knee, as compared to controls. As a whole, these data suggested that the analgesia provided by a GG-derived galactomannan depends on the chemical structure of the compound, which was also shown to be chondroprotective, questioning that the rheological properties of polysaccharides are responsible for the therapeutic benefit of “viscosupplements” (5). …”

Section: Structural Properties Of the Polysaccharides Used In Vsmentioning

confidence: 99%

“…The observation that the clinical relief after a single injection of this compound may last up to 12 months (5, 6) argues against a pure rheological effect to explain the analgesia considering that exogenous HA possibly lasts less than 1 day in the joint (5). The possibility that intra-articular hyaluronic acid (IA-HA) has disease modifying properties does also question a mechanical action as the only explanation for the therapeutic benefit.…”

Section: Introductionmentioning

confidence: 99%

“…According to some reports, the therapeutic efficacy of hylans (HA derivatives) is directly associated with their viscoelastic properties, related to their HMW and gel formulation, despite a lack of studies to prove this assumption (5, 8). …”

Section: Introductionmentioning

confidence: 99%

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Polysaccharides As Viscosupplementation Agents: Structural Molecular Characteristics but Not Rheology Appear Crucial to the Therapeutic Response

2017

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IntroductionMost clinical studies and basic research document viscosupplementation (VS) in terms of effectiveness and safety, but only a few highlight its molecular mechanisms of action. Besides, there is generally focus on hyaluronic acid (HA) as being the most relevant polysaccharide to reach the clinical endpoints, attributing its effect mainly to its unique viscoelastic properties, related to a high-molecular weight and gel formulation. Usually, studies do not approach the possible biological pathways where HA may interfere, and there is a lack of reports on other biocompatible polysaccharides that could be of use in VS.AimWe briefly review the main proposed mechanisms of action of intra-articular hyaluronic acid (IA-HA) treatment and discuss its effectiveness focusing on the role of rheological and intrinsic structural molecular properties of polysaccharides in providing a therapeutic effect.MethodsWe conducted a literature search using PubMed database to find articles dealing with the mechanisms of action of IA-HA treatment and/or emphasizing how the structural properties of the polysaccharide used influenced the clinical outcomes.Discussion/conclusionHA is involved in numerous biochemical interactions that may explain the clinical benefits of VS, most of them resulting from HA–cluster of differentiation 44 receptor interaction. There are other important aspects apart from the molecular size or the colloidal state of the IA-HA involved in VS efficiency that still need to be consolidated. Indeed, it seems that clinical response may be dependent on the intrinsic properties of the polysaccharide, regardless of being HA, rather than to rheology, posing some controversy to previous beliefs.

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Galactomannan of Delonix regia seeds reduces nociception and morphological damage in the rat model of osteoarthritis induced by sodium monoiodoacetate

Nascimento

Bringel

Maia

et al. 2020

Naunyn-Schmiedeberg's Arch Pharmacol

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Structural characteristics are crucial to the benefits of guar gum in experimental osteoarthritis

Cited by 20 publications

References 21 publications

Hylan G-F20 and galactomannan joint flares are associated to acute synovitis and release of inflammatory cytokines

Hylan G-F20 and galactomannan joint flares are associated to acute synovitis and release of inflammatory cytokines

Polysaccharides As Viscosupplementation Agents: Structural Molecular Characteristics but Not Rheology Appear Crucial to the Therapeutic Response

Galactomannan of Delonix regia seeds reduces nociception and morphological damage in the rat model of osteoarthritis induced by sodium monoiodoacetate

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