Structural characterisation and antioxidant properties of polysaccharides from the fruiting bodies of Russula virescens

Sun, Zhongwei; Zhang, Lixiang; Bin, Zhang; Tian-gui, Niu

doi:10.1016/j.foodchem.2009.05.036

Cited by 59 publications

(47 citation statements)

References 21 publications

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“…The hot water extraction yield of polysaccharides from Ganoderma tsugae showed a higher yield from mycelia than did fruit bodies, as in our result (Tseng, Yang, & Mau, 2008). However, yields of hot water-extracted crude polysaccharides from mycelia in the present work were a bit less than those from G. tsugae reported by Tseng et al (2008), which were 8.3%, whereas the yields of polysaccharide from fruit bodies in our work were clearly higher than those from fruit bodies of other species, such as Pleurotus ostreatus, 3.32% (Tong et al, 2009), Russula virescens, 1.94% (Sun, Zhang, Zhang, & Niu, 2010) and G. tsugae, 1.5-1.7% (Tseng et al, 2008). The crude polysaccharide yields from mycelia of L. polychrous Lév.…”

Section: Crude Polysaccharide Yieldscontrasting

confidence: 62%

Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév.

et al. 2011

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Section: Crude Polysaccharide Yieldscontrasting

confidence: 62%

Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév.

et al. 2011

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“…The quenching activities of Rusalan were found to be 25.29%, 39.88%, and 52.5% at the concentrations of 500, 1000 and 1500 μg/ml respectively. On comparison to previous publications, the extract exhibited better radical scavenging potentiality than that of crude polysaccharides extracted from Auricularia auricula , Agaricus bisporus , Lentinus edodes and Flammulina velutipes [61]. DPPH .…”

Section: Resultsmentioning

confidence: 67%

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

et al. 2017

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Mushrooms have been prized by humankind as medicine and culinary wonder since antiquity. Though several species are ethnically valued; many prospective species are still being discovered. One such wild macrofungus has recently been discovered during subsequent field surveys in West Bengal, India which in turn exposed as a traditionally consumed popular myco-food. The collected taxon was found to be unique with regard to its morphological as well as genetical features. After detailed characterizations, the fungus was identified as a novel taxon belonging to the genus Russula (Russulaceae, Basidiomycota). Besides, the investigation was further extended in search of new functional ingredients and in this context, a water soluble crude polysaccharide rich extract (Rusalan) was isolated from dried basidiocarps. Accumulating evidences from GC-MS, HPTLC, FT-IR along with several spectrophotometric methods postulated that the fraction consisted mainly of carbohydrate in triple helical conformation, where glucose was the major monosaccharide mostly with β-type glycosidic linkage. Conversely, Rusalan showed pronounced antioxidant activity in six in vitro assay systems with EC50 value ranging from 190–1328 μg/ml concentration. The crude polysaccharide was also evaluated against six bacterial strains using microdilution method and the growth of Staphylococcus aureus and Bacillus subtilis were found to be inhibited effectively. In addition, immune-stimulatory assays demonstrated that Rusalan could evidently promote proliferation, induce phagocytosis, release NO, produce intracellular ROS and upregulate mRNA expression of iNOS, TNF-α, COX-2, as well as IL-6 genes in in mouse macrophage cells. Therefore, aim of the present study was not only to describe a new taxon to the world mycoflora but also to introduce a potent therapeutic agent that could be explored for food and pharmaceutical purposes. However, isolation of active component and in vivo studies need to be designed further.

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“…Thus, it could be assumed that the remarkable antioxidant effect of Rusenan was not due to a single element but the result of combination of many factors. Consequently, comparative study revealed that the fraction from R. senecis might possess stronger radical scavenging potential than crude as well as pure polysaccharides extracted from R. virescens (Sun et al, 2010). The data also implied that Rusenan exhibited better chelating ability than polysaccharidic extract from P. ostreatus (Mitra et al, 2013) and P. florida (Saha et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Water Soluble Antioxidative Crude Polysaccharide From Russula senecis Elicits TLR Modulated NF-κB Signaling Pathway and Pro-inflammatory Response in Murine Macrophages

Khatua

Acharya

2018

Front. Pharmacol.

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Russula senecis has recently been reported as a new addition to macrofungal flora of West Bengal. Besides, it also emerged as a seasonal health promoting nutrient to local ethnic people and enlisted for the first time as tribal food in our previous publication. In this context, the present work was designed to establish such usefulness scientifically and to meet the aim, crude polysaccharide, Rusenan, was prepared using conventional heated water reflux. Initially, the polymers were characterized to determine chemical composition and for that spectrophotometry along with Fourier-transform infrared spectroscopy (FT-IR), high-performance thin-layer chromatography (HPTLC), and gas chromatography-mass spectrometry (GC-MS) were performed. Analysis indicated that Rusenan was consisted mainly of carbohydrate conjugated with trace amount of protein. Furthermore, glucose was detected as the major monosaccharide (mainly in β-type glycosidic linkage) while other monomers were presented in the order of galactose > mannose > xylose > rhamnose. Conversely, antioxidant potential was determined following eight in vitro systems where the fraction evidenced strong superoxide, hydroxyl, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, high affinity to Fe2+ as well as instant ability to donate electron with EC50 values ranging from 80 to 3885 μg/ml concentration. In addition, effect on murine macrophages was also investigated where the polysaccharide treatment increased cell proliferation, phagocytic activity, filopodia or lamellipodia formation, nitric oxide (NO) production and reactive oxygen species (ROS) synthesis. Thereafter, through reverse transcriptase polymerase chain reaction (RT-PCR) analysis, significant increase in the expression of Toll like receptor (TLR)-4, TLR-2 and nuclear factor kappa B (NF-κB) was observed; as a result alleviated level of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, IκB-α, and interferon (IFN)-γ were also noticed explaining definite immune-stimulatory activity of the fraction. Thus, overall finding suggests that R. senecis can be considered as a functional food and may be used in preparation of dietary supplement to enhance general health.

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Structural characterisation and antioxidant properties of polysaccharides from the fruiting bodies of Russula virescens

Cited by 59 publications

References 21 publications

Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév.

Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév.

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

Water Soluble Antioxidative Crude Polysaccharide From Russula senecis Elicits TLR Modulated NF-κB Signaling Pathway and Pro-inflammatory Response in Murine Macrophages

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