“…Measurements of absolute molecular masses and gyration radiuses of investigated samples were performed by means of high pressure size exclusion chromatography (HPSEC) as described by Nowak et al [24] HPSEC system with a multiangle laser light scattering (MALLS) detector and a differential refractive index detector (RI) was used to determine the molecular weight (M w ) and radius of gyration (R g ) of the starch polysaccharide molecules. The system consisted of a pump (Ultimate 3000, Dionex, Palo Alto, CA), an injection valve (model 7021, Rheodyne, Palo Alto, CA), a guard column (TSK PWH, Tosoh Corporation, Tokyo, Japan), and two connected size exclusion columns: TSKgel GM-PWXL (300 × 7.8 mm, Tosoh Corporation, Tokyo, Japan) and TSKgel 2500 PWXL (300 × 7.8 mm, Tosoh Corporation, Tokyo, Japan).…”