Structural basis for the magnesium-dependent activation of transketolase from Chlamydomonas reinhardtii

Pasquini, Miriam; Fermani, Simona; Tedesco, Daniele; Sciabolini, Chiara; Crozet, Pierre; Naldi, Marina; Henri, Julien; Vothknecht, Ute C.; Bertucci, Carlo; Lemaire, Stéphane D.; Zaffagnini, Mirko; Francia, Francesco

doi:10.1016/j.bbagen.2017.05.021

Cited by 11 publications

(7 citation statements)

References 76 publications

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“…29 Transketolase that carried the catalytic site (TK) is concerned with non-oxidative carbohydrate transformation of the pentose phosphate pathway and has broad substrate specificity, including xylulose 5-phosphate, erythrulose 4-phosphate, sedoheptulose 7-phosphate, etc. 30 TK plays an important role in inorganic carbon fixation through the Calvin cycle in the chloroplast of higher plants and can act as a bioconversion tool in the enzymatic synthesis of ketoses. 31 The chlorophyll a−b binding protein is a light-harvesting complex, which functions as a light receptor to capture and deliver excitation energy to photosystems.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…ATP synthase subunit α is a part of the proton-transporting ATP synthase complex, which is a catalytic core, involved in the ATP metabolic process and nucleotide binding in mitochondrion and participating in proton-transporting ATP synthase activity . Transketolase that carried the catalytic site (TK) is concerned with non-oxidative carbohydrate transformation of the pentose phosphate pathway and has broad substrate specificity, including xylulose 5-phosphate, erythrulose 4-phosphate, sedoheptulose 7-phosphate, etc . TK plays an important role in inorganic carbon fixation through the Calvin cycle in the chloroplast of higher plants and can act as a bioconversion tool in the enzymatic synthesis of ketoses .…”

Section: Resultsmentioning

confidence: 99%

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Investigation of Differentially Expressed Proteins Induced by Alteration of Natural Se Uptake with Ultrahigh-Performance Liquid Chromatography Quadrupole Orbitrap Uncovers the Potential Nutritional Value in Se-Enriched Green Tea

Jia

Liu

Shi

et al. 2020

J. Agric. Food Chem.

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Se-enriched green tea, with an increasing consumption, is the shoot of tea plants grown naturally in a seleniferous region. A label-free proteomic strategy based on ultrahigh-performance liquid chromatography quadrupole Orbitrap was applied to characterize and distinguish the difference between the Se-enriched and normal green tea with a total of 283 proteins identified and 264 proteins quantified, in which 96 proteins were observed different. The expressions of 10 proteins were upregulated and 40 proteins were downregulated (p < 0.05) in Se-enriched samples. Gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and protein–protein interaction (PPI) network analysis results indicated that these differentially expressed proteins significantly interacted and were involved in secondary metabolites and inflammatory response biological processes. Furthermore, the expression of methyl-jasmonate- and ethylene-related genes changed significantly in Se-enriched green tea, and catalase proteins were employed as the center of the pathway that changed significantly in the PPI network. These results associating with the current knowledge of selenium in soil–plant cycling revealed that organic selenium was synthesized in green tea, which provided novel information on Se assimilation in Camellia sinensis and improved the understanding of Se-enriched green tea as a possible ideal selenium supplement in daily life.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Investigation of Differentially Expressed Proteins Induced by Alteration of Natural Se Uptake with Ultrahigh-Performance Liquid Chromatography Quadrupole Orbitrap Uncovers the Potential Nutritional Value in Se-Enriched Green Tea

Jia

Liu

Shi

et al. 2020

J. Agric. Food Chem.

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“…Residues are numbered according to Uniprot reference sequences (ID: A0A2K3DSC9). CrTRXf2 was cloned in a modified pET-3d vector containing additional codons upstream of the Nco I site to express a His-tagged protein with six N-terminal histidines [41]. The expression vector was then used to transform E. coli BL21 Rosetta TM 2 (DE3) (Novagen).…”

Section: Methodsmentioning

confidence: 99%

Crystal Structure of Chloroplastic Thioredoxin f2 from Chlamydomonas reinhardtii Reveals Distinct Surface Properties

et al. 2018

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Protein disulfide reduction by thioredoxins (TRXs) controls the conformation of enzyme active sites and their multimeric complex formation. TRXs are small oxidoreductases that are broadly conserved in all living organisms. In photosynthetic eukaryotes, TRXs form a large multigenic family, and they have been classified in different types: f, m, x, y, and z types are chloroplastic, while o and h types are located in mitochondria and cytosol. In the model unicellular alga Chlamydomonas reinhardtii, the TRX family contains seven types, with f- and h-types represented by two isozymes. Type-f TRXs interact specifically with targets in the chloroplast, controlling photosynthetic carbon fixation by the Calvin–Benson cycle. We solved the crystal structures of TRX f2 and TRX h1 from C. reinhardtii. The systematic comparison of their atomic features revealed a specific conserved electropositive crown around the active site of TRX f, complementary to the electronegative surface of their targets. We postulate that this surface provides specificity to each type of TRX.

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“…TK is involved in both the pentose phosphate pathway and the Calvin-Benson Cycle, although the direction of the reaction is typically opposite in these two processes [33]. Because metabolites are extracted from all cellular compartments, it is nominally difficult to make conclusions about the specific directionality and reaction rates in each of these TK-dependent pathways.…”

Section: Impacts Of Thiamine and Hmp On Algal Central Metabolism In Amentioning

confidence: 99%

Impact of thiamine metabolites and spent medium from Chlorella sorokiniana on metabolism in the green algae Auxenochlorella prototheciodes

Higgins

Wang

et al. 2018

Algal Research

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Auxenochlorella protothecoides is a known thiamine auxotroph but our past work has shown that it can synthesize thiamine if provided with the precursor molecule 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). Partial thiamine auxotrophy is common in microalgae with important ramifications for global phytoplankton productivity as well as engineering applications of algae. While thiamine deficiency can greatly depress algae growth and lipid content, the detailed metabolic impacts of thiamine deficiency are not well understood. We used metabolomics to study the response to thiamine-limited and replete conditions in mixotrophic A. protothecoides. We also investigated the impacts of exogenous HMP addition and the use of spent medium from another green algae, C. sorokiniana, as a source of thiamine metabolites. This is the first study, to our knowledge, that addresses metabolic impacts of thiamine deficiency and alleviation in green microalgae. Thiamine deficient cultures exhibited accumulation of pyruvate and α-ketoglutarate, indicating bottlenecks at the pyruvate dehydrogenase (PDH) and oxoglutarate dehydrogenase (OGDH) complexes. Both PDH and OGDH require thiamine pyrophosphate (TPP) as a cofactor. Transketolase also requires TPP but we only observed build-up of ribose-5-phosphate when glucose was supplied as a substrate. As expected, thiamine and HMP addition could alleviate these metabolic bottlenecks while greatly increasing algal growth, neutral lipid and starch content. Spent medium from C. sorokiniana only appeared to partially alleviate thiamine deficiency and resulted in build-up of isocitrate and glycolate, metabolites that appeared relatively unaffected by the presence or absence of thiamine. Interestingly, longer culture time of C. sorokiniana when preparing the spent medium led to much higher availability of thiamine metabolites. Thus, under the right conditions, it may be possible to co-culture mutually beneficial algae species and/or recycle spent cultivation medium to overcome auxotrophy in algae.

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Structural basis for the magnesium-dependent activation of transketolase from Chlamydomonas reinhardtii

Cited by 11 publications

References 76 publications

Investigation of Differentially Expressed Proteins Induced by Alteration of Natural Se Uptake with Ultrahigh-Performance Liquid Chromatography Quadrupole Orbitrap Uncovers the Potential Nutritional Value in Se-Enriched Green Tea

Investigation of Differentially Expressed Proteins Induced by Alteration of Natural Se Uptake with Ultrahigh-Performance Liquid Chromatography Quadrupole Orbitrap Uncovers the Potential Nutritional Value in Se-Enriched Green Tea

Crystal Structure of Chloroplastic Thioredoxin f2 from Chlamydomonas reinhardtii Reveals Distinct Surface Properties

Impact of thiamine metabolites and spent medium from Chlorella sorokiniana on metabolism in the green algae Auxenochlorella prototheciodes

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