Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland

Siwińska, Małgorzata; Levina, Evgeniya A.; Shashkov, Alexander S.; Kalinchuk, N. A.; Drzewiecka, Dominika; Knirel, Yuriy A.

doi:10.1016/j.carres.2019.107831

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…A total of the 580 S strains (possessing an O antigen in the LPS molecules) were serologically investigated to classify them into proper O serogroups in the genus Proteus. The results for 24 of these strains classi ed to O2, O8, O11, O77 -O82 serogroups, respectively, had been reported previously [13][14][15][16][17][18][19][20] .…”

Section: Serological Featuressupporting

confidence: 78%

“…strains were collected in Łódź, Poland (Fig. 1) including the 24 clinical strains reported earlier [13][14][15][16][17][18][19][20]. What is worth noticing, no strains were collected from blood, although Proteus spp.…”

Section: Discussionmentioning

confidence: 99%

“…clinical isolates from the Łódź region (central Poland) enabled us to study their serological differentiation. Up to date, serologically unique strains from the collection have formed six new serogroups O77-O82 [13][14][15][16][17][18] and two new subgroups in O8 and O11 serogroups [19,20]. In this work the serological variety of the whole collection of Proteus spp.…”

Section: Introductionmentioning

confidence: 96%

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The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

Drzewiecka

Palusiak

Siwińska

et al. 2021

Preprint

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In the years 2006-2011, 617 Proteus spp. strains isolated mostly from urine and wounds or other clinical sources from infected individuals were collected in Łódź, Poland. P. mirabilis species was dominating (86.9%), followed by P. genomospecies, P. vulgaris , and P. penneri . Ninety four per cent strains were recognized as S (smooth) forms. P. mirabilis exhibited the most intensive swarming growth. Serological studies (involving ELISA – enzyme-linked immunosorbent assay and Western blotting using native and adsorbed rabbit antisera) enabled classification of 80% S isolates into respective Proteus O serogroups among the 83 ones, described so far. The remaining strains seemed to be serologically unique. Detailed serological analysis confirmed that the recently described in Poland O78 serogroup is the most widespread. Also, 14 other serogroups have been found to predominate, being represented by ten or more strains. No unique structural feature of the prevalent O serotypes has been indicated. The observed big serological variety may suggest a low rate of Proteus spp. strains’ transmission between patients or rapid changes in their lipopolysaccharides. However, the prevalence of some O serogroups indicates that particular serotypes may be in some ways beneficial to the strains producing these kinds of O antigen.

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Section: Serological Featuressupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

Drzewiecka

Palusiak

Siwińska

et al. 2021

Preprint

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“…That might be due to the low expression of these genes and/or correspondence to the significant role of other genes in this type of motility (Belas, 1994;Belas et al, 1995;Furness et al, 1997;Dufour et al, 1998;Morgenstein et al, 2010;Fusco et al, 2017;Little et al, 2018). The appearance of the demarcation line between two bacterial isolates causes a competition between them and is considered as a lack of kinship between neighboring bacterial strains (Pfaller et al, 2000;Munson et al, 2002;Drzewiecka et al, 2010;Siwińska et al, 2019). We noticed that the strains with more expansive swarming were related to less of the other strains in the Dienes test.…”

Section: Discussionmentioning

confidence: 67%

Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains

et al. 2020

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Proteus mirabilis is the third most common etiological factor of urinary tract infection. It produces urease, which contributes to the formation of a crystalline biofilm, considered to be one of the most important virulence factors of P. mirabilis strains, along with their ability to swarm on a solid surface. The aim of this study was to analyze the pathogenic properties of two selected groups of clinical P. mirabilis isolates, antimicrobial susceptible and multidrug resistant (MDR), collected from hospitals in different regions in Poland. The strains were examined based on virulence gene profiles, urease and hemolysin production, biofilm formation, and swarming properties. Additionally, the strains were characterized based on the Dienes test and antibiotic susceptibility patterns. It turned out that the MDR strains exhibited kinship more often than the susceptible ones. The strains which were able to form a stronger biofilm had broader antimicrobial resistance profiles. It was also found that the strongest swarming motility correlated with susceptibility to most antibiotics. The correlations described in this work encourage further investigation of the mechanisms of pathogenicity of P. mirabilis.

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“…clinical isolates from the Łódź region (central Poland) enabled us to study their serological differentiation. Up to date, serologically unique strains from the collection have formed six new serogroups O77-O82 13 – 18 and two new subgroups in O8 and O11 serogroups 19 , 20 . In this work the serological variety of the whole collection of Proteus spp.…”

Section: Introductionmentioning

confidence: 99%

The prevailing O serogroups among the serologically differentiated clinical Proteus spp. strains in central Poland

Drzewiecka

Palusiak

Siwińska

et al. 2021

Sci Rep

Self Cite

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In the years 2006–2011, 617 Proteus spp. strains isolated mostly from urine and wounds or other clinical sources were collected in Łódź, Poland, to determine the offensive O serotypes frequently occurring among patients. P. mirabilis exhibited the most intensive swarming growth and was dominating species (86.9%), followed by P. genomospecies, P. vulgaris, and P. penneri. Ninety four per cent strains were recognized as S (smooth) forms. Serological studies (involving ELISA—enzyme-linked immunosorbent assay and Western blotting using native and adsorbed rabbit antisera) enabled classification of 80% S isolates into respective Proteus O serogroups among the 83 ones, described so far. The remaining strains seemed to be serologically unique. Despite the observed big serological variety of Proteus spp. isolates, we found the O78 serogroup recently described in Poland as dominating and identified other widespread serotypes: O3, O6, O10, O11, O27, O28, and O30 reported earlier as predominating also in other countries; O77 and O79 detected lately in Poland; O16, O18, O20, and O50. No unique structural feature of the prevalent O serotypes has been indicated. However, the prevalence of some O serogroups indicates that particular serotypes may be in some ways beneficial to the strains producing these kinds of O antigen.

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Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland

Cited by 6 publications

References 26 publications

The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains

The prevailing O serogroups among the serologically differentiated clinical Proteus spp. strains in central Poland

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