Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains

Filipiak, Aneta; Chrapek, Magdalena; Literacka, Elżbieta; Wawszczak, Monika; Głuszek, Stanisław; Majchrzak, Michał; Wróbel, Grzegorz; Łysek-Gładysińska, Małgorzata; Gniadkowski, Marek; Adamus-Białek, Wioletta

doi:10.3389/fmicb.2020.579389

Cited by 25 publications

(17 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteus mirabilis is the 4 th cause of urinary tract infections in women [38] and males [12], and like other germs, their resistance to antimicrobials increases drastically [37]. A recent study evaluated its resistance regarding forming biofilmsstable biofilms mean higher resistance [21]. Studies in Ghana have confirmed the resistance of Proteus mirabilis to cotrimoxazole (81%) and ampicillin (77%) [20], different data from ours: cotrimoxazole 71.42% and ampicillin 28.57%.…”

Section: Resultsmentioning

confidence: 68%

Bacterial Pathogens Isolated From Surgical Site Infections and Their Antibiotic Susceptibility

Petca¹

2021

FARMACIA

View full text Add to dashboard Cite

The increased reservoir of pathogenic bacteria sheltered in hospitals is the cause of various types of nosocomial infections and represents one of the major health problems. Surgical site infections (SSIs) count for 14 -16% of all hospital-acquired infections (HAI) and represent the 3 rd most occurring nosocomial infections and the 3 rd most expensive HAI. We present a germ pattern (on 92 strains), located in post-operative dehiscence sites -treated with vacuum-assisted closure, in failed per secundam suture. The most common pathogens were E. coli -18.47% (76.47% multi-drug resistant -MDR), Pseudomonas aeruginosa -16.30% (33.33% MDR) and Enterococcus faecalis -15.21%. Acinetobacter baumannii (100%) and Klebsiella pneumoniae (88.88%) also showed multi-drug resistance. Gentamicin, amikacin and carbapenems (> 50% sensibility) are eligible for treating SSIs, whereas cephalosporins have a higher resistance rate (> 50%). Knowledge of resistance patterns in SSIs is recommendable to ensure proper empirical treatment. RezumatRezervorul crescut de bacterii patogene din spitale reprezintă cauza multiplelor tipuri de infecții nosocomiale, fiind una dintre problemele majore de sănătate. Infecțiile plăgilor postoperatorii ocupă locul 3 atât ca incidență cât și ca cea mai costisitoare infecție nosocomială. Studiul prezintă tiparul de rezistență a unei serii de patogeni (92 de bacterii) din plăgile postoperatorii dehiscente, pentru care s-a aplicat sistemul de terapie cu presiune negativă, după eșuarea suturii per secundam. Cele mai frecvente bacterii au fost E. coli -18,47% (76,47% multirezistent), Pseudomonas aeruginosa -16,30% (33,33% multirezistent) și Enterococcus faecalis -15,21%. În privința multirezistenței bacteriene s-au evidențiat și Acinetobacter baumannii (100%), Klebsiella pneumoniae (88,88%). Tratamentul cu gentamicină, amikacină și clasa carbapenemelor (sensibilitate > 50% fiecare) pot fi o soluție; terapia cu cefalosporine nu trebuie indicată de primă intenție (rezistență > 50%). Cunoașterea tiparelor de rezistență în infecțiile plăgilor operatorii este recomandabilă pentru a asigura un tratament empiric adecvat.

show abstract

Section: Resultsmentioning

confidence: 68%

Bacterial Pathogens Isolated From Surgical Site Infections and Their Antibiotic Susceptibility

Petca¹

2021

FARMACIA

View full text Add to dashboard Cite

show abstract

“…Concerning the correlation between the antimicrobial resistance genes and the virulence determinants, a previous study that was reported by Filipiak 64 revealed an inversed correlation between the virulence factors and the presence of the resistance genes in the retrieved P. mirabilis strains. However, in the present study, the majority of the screened virulence genes were found in the recovered isolates.…”

Section: Discussionmentioning

confidence: 82%

atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis

Algammal

Hashem

Alfifi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Proteus mirabilis is a common opportunistic pathogen causing severe illness in humans and animals. To determine the prevalence, antibiogram, biofilm-formation, screening of virulence, and antimicrobial resistance genes in P. mirabilis isolates from ducks; 240 samples were obtained from apparently healthy and diseased ducks from private farms in Port-Said Province, Egypt. The collected samples were examined bacteriologically, and then the recovered isolates were tested for atpD gene sequencing, antimicrobial susceptibility, biofilm-formation, PCR detection of virulence, and antimicrobial resistance genes. The prevalence of P. mirabilis in the examined samples was 14.6% (35/240). The identification of the recovered isolates was confirmed by the atpD gene sequencing, where the tested isolates shared a common ancestor. Besides, 94.3% of P. mirabilis isolates were biofilm producers. The recovered isolates were resistant to penicillins, sulfonamides, β-Lactam-β-lactamase-inhibitor-combinations, tetracyclines, cephalosporins, macrolides, and quinolones. Using PCR, the retrieved strains harbored atpD, ureC, rsbA, and zapA virulence genes with a prevalence of 100%, 100%, 94.3%, and 91.4%, respectively. Moreover, 31.4% (11/35) of the recovered strains were XDR to 8 antimicrobial classes that harbored blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Besides, 22.8% (8/35) of the tested strains were MDR to 3 antimicrobial classes and possessed blaTEM, tetA, and sul1genes. Furthermore, 17.1% (6/35) of the tested strains were MDR to 7 antimicrobial classes and harbored blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Alarmingly, three strains were carbapenem-resistant that exhibited PDR to all the tested 10 antimicrobial classes and shared blaTEM, blaOXA-1, blaCTX-M, tetA, and sul1 genes. Of them, two strains harbored the blaNDM-1 gene, and one strain carried the blaKPC gene. In brief, to the best of our knowledge, this is the first study demonstrating the emergence of XDR and MDR-P.mirabilis in ducks. Norfloxacin exhibited promising antibacterial activity against the recovered XDR and MDR-P. mirabilis. The emergence of PDR, XDR, and MDR-strains constitutes a threat alarm that indicates the complicated treatment of the infections caused by these superbugs.

show abstract

“…A high percentage of the isolates were found to be resistant to ampicillin, ampicillin/sulbactam, cefotaxime, gentamicin, and nitrofurantoin. In the literature data, there are many reports about bacterial resistance to SXT, β-lactams, fluoroquinolones, nitrofurantoin, fosfomycin, aminoglycosides, tetracyclines, and sulfonamides [ 30 , 31 , 32 , 33 ]. Due to this phenomenon, there is a need to explore other effective and alternative drugs for the treatment of UTIs and their complications.…”

Section: Discussionmentioning

confidence: 99%

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

et al. 2022

View full text Add to dashboard Cite

The natural microbiota of the urinary tract includes Lactobacillus spp., which secrete molecules with antimicrobial properties and have antagonistic activity against many pathogens. This paper focuses on the antibacterial effect of Lactobacillus strains isolated from urine against clinical strains of Proteus mirabilis isolated from kidney stones and from urine with coexisting urolithiasis. The study involved analyzing the main antimicrobial molecules secreted by Lactobacillus. In order to indicate which agent had the strongest antimicrobial effect, the supernatants were made alkaline and treated with catalase and high temperature. Both treated and untreated supernatants were analyzed for their activity. Exposing uropathogens to all untreated cell-free supernatants of Lactobacillus significantly reduced their growth, and it was established that these properties were related to organic acid secretion by these strains. Using LC–MS/MS and spectrophotometric techniques, lactic, citric, and succinic acids were determined qualitatively and quantitatively. The influence of these acids on the P. mirabilis growth and biofilm formation and their influence on membrane permeability were also investigated. The results indicate that organic acids secreted by Lactobacillus strains have a high antibacterial potential and could be used as novel agents in the treatment of urinary tract infections caused by P. mirabilis.

show abstract

Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains

Cited by 25 publications

References 85 publications

Bacterial Pathogens Isolated From Surgical Site Infections and Their Antibiotic Susceptibility

Bacterial Pathogens Isolated From Surgical Site Infections and Their Antibiotic Susceptibility

atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

Contact Info

Product

Resources

About