1976
DOI: 10.1128/jvi.18.1.227-234.1976
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Structural and growth characteristics of infectious bursal disease virus

Abstract: The infectious bursal disease virus is not enveloped and has a diameter of 60 nm and a density of about 1.32 g/ml. It contains two pieces of single-stranded RNA with molecular weights close to 2 X 10(6). The capsid is made up of four major polypeptides with molecular weights of 110,000, 50,000, 35,000, and 25,000. The virus replicates in chicken embryo fibroblasts rather than in epitheloid cells. After an eclipse period of 4 h, virus production reaches a maximum about 12 h later. The virus has no structural or… Show more

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Cited by 127 publications
(31 citation statements)
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References 17 publications
(19 reference statements)
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“…To determine the specificity of the recovered viruses, CEF cells were infected with the transfectant viruses and the infected cells were analyzed by immunofluorescence assay with rabbit anti-IBDV polyclonal serum, as described previously (25). To examine viral structural proteins expressed by recovered chimeric viruses, the viruses (including the one propagated in the CAM) were purified by sucrose gradient centrifugation and analyzed by Western blotting, as described previously (26,39). To further characterize the recovered virus, RT-PCR was performed on the chimeric virus with the appropriate primer pair, used to produce the original clone.…”
Section: Cells Viruses and Hybridomasmentioning
confidence: 99%
“…To determine the specificity of the recovered viruses, CEF cells were infected with the transfectant viruses and the infected cells were analyzed by immunofluorescence assay with rabbit anti-IBDV polyclonal serum, as described previously (25). To examine viral structural proteins expressed by recovered chimeric viruses, the viruses (including the one propagated in the CAM) were purified by sucrose gradient centrifugation and analyzed by Western blotting, as described previously (26,39). To further characterize the recovered virus, RT-PCR was performed on the chimeric virus with the appropriate primer pair, used to produce the original clone.…”
Section: Cells Viruses and Hybridomasmentioning
confidence: 99%
“…The test was repeated once, and the mean of the percentage of reactivity was calculated. In addition to the three Bangladeshi isolates, two other classical virulent strains of IBDV, F 52/70 (Bygrave and Faragher, 1970) and Cu-1wt (Nick et al, 1976;Lange et al, 1987), were also included in the antigenic analysis. Before the antigen characterization assay, the optimum concentration of each test antigen was determined.…”
Section: Ac-elisamentioning
confidence: 99%
“…An IBDV virus strain LH, purified in China, was utilized in this study. Purified IBDV was prepared as previously described [31] from the Bursa of Fabricius of chickens (BF) that had died from infectious bursal disease (IBD). The concentration of viral protein was determined according to previous report [32].…”
Section: Methodsmentioning
confidence: 99%
“…Nonpurified virus were either propagated on chicken embryo fibroblasts (CEF) in MEM medium, or prepared from tissue homogenate of BF of chickens that had died from the disease [31]. The supernatant of the infected CEF was collected when a cytopathic effect was clearly visible.…”
Section: Methodsmentioning
confidence: 99%