Structural and growth characteristics of infectious bursal disease virus

Nick, H; Cursiefen, Dagmar; Becht, H.

doi:10.1128/jvi.18.1.227-234.1976

Cited by 127 publications

(31 citation statements)

References 17 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the specificity of the recovered viruses, CEF cells were infected with the transfectant viruses and the infected cells were analyzed by immunofluorescence assay with rabbit anti-IBDV polyclonal serum, as described previously (25). To examine viral structural proteins expressed by recovered chimeric viruses, the viruses (including the one propagated in the CAM) were purified by sucrose gradient centrifugation and analyzed by Western blotting, as described previously (26,39). To further characterize the recovered virus, RT-PCR was performed on the chimeric virus with the appropriate primer pair, used to produce the original clone.…”

Section: Cells Viruses and Hybridomasmentioning

confidence: 99%

Molecular Determinants of Virulence, Cell Tropism, and Pathogenic Phenotype of Infectious Bursal Disease Virus

Brandt

Yao

Liu

et al. 2001

J Virol

216

143

View full text Add to dashboard Cite

Infectious bursal disease viruses (IBDVs), belonging to the family Birnaviridae, exhibit a wide range of immunosuppressive potential, pathogenicity, and virulence for chickens. The genomic segment A encodes all the structural (VP2, VP4, and VP3) and nonstructural proteins, whereas segment B encodes the viral RNA-dependent RNA polymerase (VP1). To identify the molecular determinants for the virulence, pathogenic phenotype, and cell tropism of IBDV, we prepared full-length cDNA clones of a virulent strain, Irwin Moulthrop (IM), and constructed several chimeric cDNA clones of segments A and B between the attenuated vaccine strain (D78) and the virulent IM or GLS variant strain. Using the cRNA-based reverse-genetics system developed for IBDV, we generated five chimeric viruses after transfection by electroporation procedures in Vero or chicken embryo fibroblast (CEF) cells, one of which was recovered after propagation in embryonated eggs. To evaluate the characteristics of the recovered viruses in vivo, we inoculated 3-week-old chickens with D78, IM, GLS, or chimeric viruses and analyzed their bursae for pathological lesions 3 days postinfection. Viruses in which VP4, VP4-VP3, and VP1 coding sequences of the virulent strain IM were substituted for the corresponding region in the vaccine strain failed to induce hemorrhagic lesions in the bursa. In contrast, viruses in which the VP2 coding region of the vaccine strain was replaced with the variant GLS or virulent IM strain caused rapid bursal atrophy or hemorrhagic lesions in the bursa, as seen with the variant or classical virulent strain, respectively. These results show that the virulence and pathogenic-phenotype markers of IBDV reside in VP2. Moreover, one of the chimeric viruses containing VP2 sequences of the virulent strain could not be recovered in Vero or CEF cells but was recovered in embryonated eggs, suggesting that VP2 contains the determinants for cell tropism. Similarly, one of the chimeric viruses containing the VP1 segment of the virulent strain could not be recovered in Vero cells but was recovered in CEF cells, suggesting that VP1 contains the determinants for cell-specific replication in Vero cells. By comparing the deduced amino acid sequences of the D78 and IM strains and their reactivities with monoclonal antibody 21, which binds specifically to virulent IBDV, the putative amino acids involved in virulence and cell tropism were identified. Our results indicate that residues Gln at position 253 (Gln253), Asp279, and Ala284 of VP2 are involved in the virulence, cell tropism, and pathogenic phenotype of virulent IBDV.

show abstract

Section: Cells Viruses and Hybridomasmentioning

confidence: 99%

Molecular Determinants of Virulence, Cell Tropism, and Pathogenic Phenotype of Infectious Bursal Disease Virus

Brandt

Yao

Liu

et al. 2001

J Virol

216

143

View full text Add to dashboard Cite

show abstract

“…The test was repeated once, and the mean of the percentage of reactivity was calculated. In addition to the three Bangladeshi isolates, two other classical virulent strains of IBDV, F 52/70 (Bygrave and Faragher, 1970) and Cu-1wt (Nick et al, 1976;Lange et al, 1987), were also included in the antigenic analysis. Before the antigen characterization assay, the optimum concentration of each test antigen was determined.…”

Section: Ac-elisamentioning

confidence: 99%

Molecular and Antigenic Characterization of Bangladeshi Isolates of Infectious Bursal Disease Virus Demonstrate their Similarities with Recent European, Asian and African Very Virulent Strains

Islam

Zierenberg

Eterradossi

et al. 2001

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

Three isolates of infectious bursal disease virus (IBDV), obtained from chickens in Bangladesh in 1999 and designated as BD 1/99, BD 2/99 and BD 3/99, were characterized. In an antigen capture enzyme-linked immunosorbent assay using a panel of VP2-specific neutralizing monoclonal antibodies (mAb), all three isolates showed a mAb-binding profile similar to that of very virulent IBDV (vvIBDV) strains. In contrast to the classical virulent strains, they did not react with mAb 3 and mAb 4. Molecular characterization was performed by direct sequencing of a 677-base pair cDNA corresponding to the VP2 variable domain of the polyprotein gene, synthesized by a reverse transcription-polymerase chain reaction. In comparison to the classical virulent strains, the Bangladeshi isolates were found to have five amino acid substitutions in this region. Four of these changes, Pro222Ala, Val256Ile, Leu294Ile and Asn299Ser, were also observed in other vvIBDV strains. The fifth substitution, Glu300Ala, was similar to that in some African strains of IBDV. The results support the observation that antigenically and genetically similar vvIBDV strains, first observed in Europe in the late 1980s, have spread to most parts of the world in a short period of time.

show abstract

“…An IBDV virus strain LH, purified in China, was utilized in this study. Purified IBDV was prepared as previously described [31] from the Bursa of Fabricius of chickens (BF) that had died from infectious bursal disease (IBD). The concentration of viral protein was determined according to previous report [32].…”

Section: Methodsmentioning

confidence: 99%

“…Nonpurified virus were either propagated on chicken embryo fibroblasts (CEF) in MEM medium, or prepared from tissue homogenate of BF of chickens that had died from the disease [31]. The supernatant of the infected CEF was collected when a cytopathic effect was clearly visible.…”

Section: Methodsmentioning

confidence: 99%

Effects of hydrostatic pressure on the structure and biological activity of infectious bursal disease virus

Tian

Ruan

Qian

et al. 2000

European Journal of Biochemistry

View full text Add to dashboard Cite

The effects of high hydrostatic pressure on the structure and biological activity of infectious bursal disease virus (IBDV), a commercially important pathogen of chickens, were investigated. IBDV was completely dissociated into subunits at a pressure of 240 MPa and 0 8C revealed by the change in intrinsic fluorescence spectrum and light scattering. The dissociation of IBDV showed abnormal concentration dependence as observed for some other viruses. Electron microscopy study showed that morphology of IBDV had an obvious change after pressure treatment at 0 8C. It was found that elevating pressure destroyed the infectivity of IBDV, and a completely pressure-inactivated IBDV could be obtained under proper conditions. The pressure-inactivated IBDV retained the original immunogenic properties and could elicit high titers of virus neutralizing antibodies. These results indicate that hydrostatic pressure provides a potential physical means to prepare antiviral vaccine.

show abstract

Structural and growth characteristics of infectious bursal disease virus

Cited by 127 publications

References 17 publications

Molecular Determinants of Virulence, Cell Tropism, and Pathogenic Phenotype of Infectious Bursal Disease Virus

Molecular Determinants of Virulence, Cell Tropism, and Pathogenic Phenotype of Infectious Bursal Disease Virus

Molecular and Antigenic Characterization of Bangladeshi Isolates of Infectious Bursal Disease Virus Demonstrate their Similarities with Recent European, Asian and African Very Virulent Strains

Effects of hydrostatic pressure on the structure and biological activity of infectious bursal disease virus

Contact Info

Product

Resources

About