Effects of hydrostatic pressure on the structure and biological activity of infectious bursal disease virus

Abstract

The effects of high hydrostatic pressure on the structure and biological activity of infectious bursal disease virus (IBDV), a commercially important pathogen of chickens, were investigated. IBDV was completely dissociated into subunits at a pressure of 240 MPa and 0 8C revealed by the change in intrinsic fluorescence spectrum and light scattering. The dissociation of IBDV showed abnormal concentration dependence as observed for some other viruses. Electron microscopy study showed that morphology of IBDV had an obvious change after pressure treatment at 0 8C. It was found that elevating pressure destroyed the infectivity of IBDV, and a completely pressure-inactivated IBDV could be obtained under proper conditions. The pressure-inactivated IBDV retained the original immunogenic properties and could elicit high titers of virus neutralizing antibodies. These results indicate that hydrostatic pressure provides a potential physical means to prepare antiviral vaccine.

New Insights into the Mechanisms of Protein Misfolding and Aggregation in Amyloidogenic Diseases Derived from Pressure Studies

“…The observed effects vary from small perturbations in the conformation of the envelope proteins to complete disassembly of the virus capsid (40)(41)(42)(43). In all these studies, highly specific protein-protein and protein-nucleic acid interactions are the targets for the high-pressure perturbation.…”

Full Inactivation of Human Influenza Virus by High Hydrostatic Pressure Preserves Virus Structure and Membrane Fusion While Conferring Protection to Mice against Infection

“…Pressure-based virus inactivation is a promising alternative and an industrially mature technology. Some groups have successfully inactivated human and animal viruses using high hydrostatic pressure (HHP), and satisfactory immune responses have been produced after vaccination and challenge [10-12]. …”

“…Viruses that have been successfully inactivated by HHP include vesicular stomatitis virus [11], yellow fever virus [15], avian influenza viruses [10,19], Hepatitis A virus [20], norovirus [21], and infectious bursal disease virus [12]. HHP has the potential to cause viral inactivation without drastically affecting viral immunogenic properties or destroying structural epitopes [10,11,19,22].…”

“…This interesting finding highlights the potential application of this tool to prepare whole viral vaccines in a simple, fast, and inexpensive way. Moreover, this approach would not introduce exogenous substances into vaccines, which differs from inactivation using chemical methods [15,12,11]. …”

Effects of high hydrostatic pressure on Eimeria acervulina pathogenicity, immunogenicity and structural integrity

“…Several microbial pathogens that can be inactivated by high hydrostatic pressure have also been demonstrated to retain immunogenic properties, including Leptospira interrogans (Silva et al, 2001), Vesicular Stomatitis Virus (Silva et al, 1992), Infectious Bursal Disease Virus (Tian et al, 2000), Foot and Mouth Disease Virus (Ishimaru et al, 2004), and Coxsackievirus group B (Chen, Tian, & Ruan, 2001), and thus, development of a vaccine by high hydrostatic pressure is a theoretical possibility. Having established that high pressure can render E. acervulina non-pathogenic, we next explored whether immunogenic properties of the parasite were retained after pressure treatment and whether potential for vaccine development by high-hydrostatic pressure exists.…”