Structural and genetic characterization of the O-antigen of Enterobacter cloacae C5529 related to the O-antigen of E. cloacae G3054

Han, Richou; Perepelov, Andrei V.; Wang, Yuanyuan; Filatov, Andrei V.; Wang, Min; Shashkov, Alexander S.; Wang, Lei; Knirel, Yuriy A.

doi:10.1016/j.carres.2017.02.006

Cited by 7 publications

(2 citation statements)

References 19 publications

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“…The O-AGC of E. cloacae has been reported to be located between two housekeeping genes, galF and gnd, and shows perfect correlations with each O-antigen structure in several isolates (Filatov et al, 2014;Perepelov et al, 2014Perepelov et al, , 2015Perepelov et al, , 2016Perepelov et al, , 2017Han et al, 2017). These previous studies shed clear genetic and evolutionary information regarding O-AGC of E. cloacae.…”

Section: Introductionmentioning

confidence: 58%

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Huang

Wang

et al. 2020

Front. Microbiol.

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Enterobacter cloacae is a well-characterized opportunistic pathogen that is closely associated with various nosocomial infections. The O-antigen, which is one of the most variable constituents on the cell surface, has been used widely and traditionally for serological classification of many gram-negative bacteria. E. cloacae is divided into 30 serotypes, based on its O-antigen diversity. In this study, by using genomic and comparative-genomic approaches, we analyzed the O-antigen gene clusters of 26 E. cloacae serotypes in depth. We also identified the sero-specific gene for each serotype and developed a multiplex polymerase chain reaction (PCR) method. The sensitivity of the assay was 0.1 ng for genomic DNA and 10 3 colony forming units for pure cultures. The assay reliability was evaluated by double-blinded testing with 81 clinical strains. Furthermore, we established a valid, genome-based tool for in silico serotyping of E. cloacae. By screening 431 E. cloacae genomes deposited in GenBank, 304 were classified into current antigenic scheme, and 112 were allocated into 55 putative novel serotypes. Our results represent the first genetic basis of the O-antigen diversity and variation of E. cloacae, providing a rationale for studying the O-antigen associated evolution and pathogenesis of this bacterium. In addition, we extended the current serotyping system for E. cloacae, which is important for detection and epidemiological surveillance purposes for this important pathogen.

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Section: Introductionmentioning

confidence: 58%

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Huang

Wang

et al. 2020

Front. Microbiol.

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“…[2] Belonging to the nonulosonic acid carbohydrate family, Pse is structurally related to its well-known congener, sialic acid. Anomerically, Pse exists with both α [3][4][5][6][7] and β [8][9][10][11][12][13] configuration in native glycoconjugates with variable substitution patterns at N5 and N7. As illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

A Solution to Chemical Pseudaminylation via Development of a Bimodal Glycosyl Donor to Enable Highly Stereocontrolled α- and β-Glycosylation

Wei¹,

Liu²,

Tang³

et al. 2019

Preprint

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Bacterial pseudaminic acids (Pse) are present on the surface of many pathogenic bacteria. Herein, we report a robust methodology for the stereocontrolled chemical glycosylation of pseudaminic acid to afford both α- (axial) and β- (equatorial) glycosides reliably with complete stereoselectivity, using a common glycosyl donor (7N-Cbz/5N-azido Pse thioglycoside) simply by changing the reaction conditions (DCM-DMF, -40 oC and DCM/MeCN, -78 oC, respectively). Examples of such bimodal selectivity are both sparse and highly sought-after in carbohydrate chemistry. This method enables efficient access to pseudaminylated molecules, which will open up various opportunities in chemical glycobiology research of bacterial pseudaminic acids and carbohydrate-based antibacterial vaccine development.

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Structure and gene cluster of the O-antigen of Enterobacter cloacae C4115

Perepelov

Zhang

et al. 2017

Carbohydrate Research

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Structural and genetic characterization of the O-antigen of Enterobacter cloacae C5529 related to the O-antigen of E. cloacae G3054

Cited by 7 publications

References 19 publications

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

A Solution to Chemical Pseudaminylation via Development of a Bimodal Glycosyl Donor to Enable Highly Stereocontrolled α- and β-Glycosylation

Structure and gene cluster of the O-antigen of Enterobacter cloacae C4115

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