Structure and gene cluster of the O-antigen of Enterobacter cloacae C4115

Perepelov, Andrei V.; Xu, Guangnan; Zhang, Xinjie; Shashkov, Alexander S.; Wang, Min; Knirel, Yuriy A.

doi:10.1016/j.carres.2017.05.021

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…The O-AGC of E. cloacae has been reported to be located between two housekeeping genes, galF and gnd, and shows perfect correlations with each O-antigen structure in several isolates (Filatov et al, 2014;Perepelov et al, 2014Perepelov et al, , 2015Perepelov et al, , 2016Perepelov et al, , 2017Han et al, 2017). These previous studies shed clear genetic and evolutionary information regarding O-AGC of E. cloacae.…”

Section: Introductionmentioning

confidence: 58%

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Huang

Wang

et al. 2020

Front. Microbiol.

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Enterobacter cloacae is a well-characterized opportunistic pathogen that is closely associated with various nosocomial infections. The O-antigen, which is one of the most variable constituents on the cell surface, has been used widely and traditionally for serological classification of many gram-negative bacteria. E. cloacae is divided into 30 serotypes, based on its O-antigen diversity. In this study, by using genomic and comparative-genomic approaches, we analyzed the O-antigen gene clusters of 26 E. cloacae serotypes in depth. We also identified the sero-specific gene for each serotype and developed a multiplex polymerase chain reaction (PCR) method. The sensitivity of the assay was 0.1 ng for genomic DNA and 10 3 colony forming units for pure cultures. The assay reliability was evaluated by double-blinded testing with 81 clinical strains. Furthermore, we established a valid, genome-based tool for in silico serotyping of E. cloacae. By screening 431 E. cloacae genomes deposited in GenBank, 304 were classified into current antigenic scheme, and 112 were allocated into 55 putative novel serotypes. Our results represent the first genetic basis of the O-antigen diversity and variation of E. cloacae, providing a rationale for studying the O-antigen associated evolution and pathogenesis of this bacterium. In addition, we extended the current serotyping system for E. cloacae, which is important for detection and epidemiological surveillance purposes for this important pathogen.

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Section: Introductionmentioning

confidence: 58%

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Huang

Wang

et al. 2020

Front. Microbiol.

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“…1 ). 4 Considering the growing need for robust and practical strategies to deal with the synthesis of bacterial cell surface oligosaccharides bearing rare amino deoxy sugar residues 5 we took up the challenge of developing a concise strategy for the synthesis of this pentasaccharide. Taking a cue from the recent reports directed towards synthesis of rare sugar derivatives, 6,7 herein we report the first total synthesis of the aforesaid pentasaccharide in the form of its p -methoxyphenyl glycoside (1, Fig.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of the pentasaccharide repeating unit of the O-antigen from Enterobacter cloacae C4115 containing the rare α-d-FucNAc

Chaudhury

Mukhopadhyay

2020

RSC Adv.

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Establishment of a suspension array for Pseudomonas aeruginosa O-antigen serotyping

Qian

et al. 2018

Journal of Microbiological Methods

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Structure and gene cluster of the O-antigen of Enterobacter cloacae C4115

Cited by 3 publications

References 35 publications

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme for Enterobacter cloacae

Synthesis of the pentasaccharide repeating unit of the O-antigen from Enterobacter cloacae C4115 containing the rare α-d-FucNAc

Establishment of a suspension array for Pseudomonas aeruginosa O-antigen serotyping

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