Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen

Groeme, Rachel; Airouche, Sabi; Kopečný, David; Jaekel, Judith; Savko, Martin; Berjont, Nathalie; Bussières, Laetitia; Mignon, Maxime Le; Jagic, Franck; Zieglmayer, Petra; Baron‐Bodo, Véronique; Floch, Véronique Bordas-Le; Mascarell, Laurent; Briozzo, Pierre; Moingeon, Philippe

doi:10.1074/jbc.m115.702001

Cited by 24 publications

(19 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In detail, the mice showed airway hyperresponsiveness, high levels of eosinophils and innate lymphoid type 2 cells (ILC2) in bronchoalveolar lavage fluid, as well as Amb a 11-specific IgE and IgG 1 responses after challenge with ragweed pollen extract. However, sensitization with pro-rAmb a 11 or E64-inhibited rAmb a 11 resulted in lower allergic responses [53]. These effects correlate with reports on cysteine protease allergen-induced disruption of airway epithelium, as well as with protease-enhanced activation of immune cells and initiation of Th2-type inflammation [54].…”

Section: Major Ragweed Pollen Allergenssupporting

confidence: 60%

See 1 more Smart Citation

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Chen

Marusciac

Tamas

et al. 2018

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Ambrosia artemisiifolia, also known as common or short ragweed, is an invasive annual flowering herbaceous plant that has its origin in North America. Nowadays, ragweed can be found in many areas worldwide. Ragweed pollen is known for its high potential to cause type I allergic reactions in late summer and autumn and represents a major health problem in America and several countries in Europe. Climate change and urbanization, as well as long distance transport capacity, enhance the spread of ragweed pollen. Therefore ragweed is becoming domestic in non-invaded areas which in turn will increase the sensitization rate. So far 11 ragweed allergens have been described and, according to IgE reactivity, Amb a 1 and Amb a 11 seem to be major allergens. Sensitization rates of the other allergens vary between 10 and 50%. Most of the allergens have already been recombinantly produced, but most of them have not been characterized regarding their allergenic activity, therefore no conclusion on the clinical relevance of all the allergens can be made, which is important and necessary for an accurate diagnosis. Pharmacotherapy is the most common treatment for ragweed pollen allergy but fails to impact on the course of allergy. Allergen-specific immunotherapy (AIT) is the only causative and disease-modifying treatment of allergy with long-lasting effects, but currently it is based on the administration of ragweed pollen extract or Amb a 1 only. In order to improve ragweed pollen AIT, new strategies are required with higher efficacy and safety.

show abstract

Section: Major Ragweed Pollen Allergenssupporting

confidence: 60%

“…The structure of pro rAmb a 11 is typical of a C1A cysteine protease consisting of 6 alpha-helices and a beta-sheet formed from 6 antiparallel beta-strands in its mature form (Fig. 4) [53]. …”

Section: Major Ragweed Pollen Allergensmentioning

confidence: 99%

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Chen

Marusciac

Tamas

et al. 2018

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…The coordinates files of SET/TAF‐Iβ and NRP1 were obtained using homology models based on PDB ID 2E50 , as described previously . The following protein structures were constructed as homology models with SWISS‐MODEL: STRAP (based on 3JAP ), eiF2γ (based on 6FEC, ), BiP1 (based on 6HAB, ), RD21 (based on 5EGW, ), TCL (based on 6ES4, ), and Sm/D1 (based on 5XJC, ).…”

Section: Cytochrome C In the Cytoplasm: Established And New Targetsmentioning

confidence: 99%

New moonlighting functions of mitochondrial cytochrome c in the cytoplasm and nucleus

et al. 2019

View full text Add to dashboard Cite

Cytochrome c (Cc) is a protein that functions as an electron carrier in the mitochondrial respiratory chain. However, Cc has moonlighting roles outside mitochondria driving the transition of apoptotic cells from life to death. When living cells are damaged, Cc escapes its natural mitochondrial environment and, once in the cytosol, it binds other proteins to form a complex named the apoptosome—a platform that triggers caspase activation and further leads to controlled cell dismantlement. Early released Cc also binds to inositol 1,4,5‐triphosphate receptors on the ER membrane, which stimulates further massive Cc release from mitochondria. Besides the well‐characterized binding proteins contributing to the proapoptotic functions of Cc, many novel protein targets have been recently described. Among them, histone chaperones were identified as key partners of Cc following DNA breaks, indicating that Cc might modulate chromatin dynamics through competitive binding to histone chaperones. In this article, we review the ample set of recently discovered antiapoptotic proteins—involved in DNA damage, transcription, and energetic metabolism—reported to interact with Cc in the cytoplasm and even the nucleus upon DNA breaks.

show abstract

“…The mouse model of papaya allergy was established following an in vivo allergen challenge protocol described in Groeme et al (2016) with certain modifications. Briefly, 6–8 weeks old female BALB/c mice were assorted into four groups (6 mice per group).…”

Section: Methodsmentioning

confidence: 99%

Cari p 1, a Novel Polygalacturonase Allergen From Papaya Acting as Respiratory and Food Sensitizer

et al. 2018

View full text Add to dashboard Cite

Papaya has been reported to elicit IgE-mediated hypersensitivity via pollen inhalation and fruit consumption. Certain papaya sensitive patients with food allergy were found to experience recurrent respiratory distresses even after quitting the consumption of fruits. This observation prompted us to investigate the allergens commonly present in fruits and pollen grains of papaya. A discovery approach consisting of immunoproteomic detection followed by molecular characterization led to the identification of a novel papaya allergen designated as Cari p 1. This allergen was detected as a 56 kDa IgE-reactive protein from pollen as well as fruit proteome through serological analysis. The protein was identified as an endopolygalacturonase by tandem mass spectrometry. Full length Cari p 1 cDNA was isolated from papaya pollen, cloned in expression vector, and purified as recombinant allergen. The recombinant protein was monomeric and displayed pectinolytic activity. Recombinant Cari p 1 reacted with IgE-antibodies of all the papaya sensitized patient sera. In addition to IgE-reactivity, rCari p 1 displayed allergenic activity by stimulating histamine release from IgE-sensitized granulocytes. CD-spectroscopy of rCari p 1 revealed the presence of predominantly β-sheet characters. The melting curve of the allergen showed partial refolding from a fully denatured state indicating the possible presence of conformational IgE-epitopes characteristic of inhalant allergens in addition to the linear IgE-epitopes of food allergens. The expression of this allergen in papaya fruits was detected by immunoblot with anti-Cari p 1 rabbit IgG and reconfirmed by PCR. In an in vivo mouse model, rCari p 1 exhibited a comparable level of inflammatory responses in the lung and duodenum tissues explaining the dual role of Cari p 1 allergen in respiratory sensitization via pollen inhalation and sensitization of gut mucosa via fruit consumption. Purified rCari p 1 can be used a marker allergen for component-resolved molecular diagnosis. Further immunological studies on Cari p 1 are warranted to design immunotherapeutic vaccine for the clinical management of papaya allergy.

show abstract

Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen

Cited by 24 publications

References 54 publications

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

New moonlighting functions of mitochondrial cytochrome c in the cytoplasm and nucleus

Cari p 1, a Novel Polygalacturonase Allergen From Papaya Acting as Respiratory and Food Sensitizer

Contact Info

Product

Resources

About