Structural and functional characterization of Tomato SUMO1 gene

Abstract

Small ubiquitin-related modifier (SUMO) genes regulate various functions of target proteins through post-translational modification. The SUMO proteins have a similar 3-dimensional structure as that of ubiquitin proteins and occur through a cascade of enzymatic reactions. In the present study we have cloned a new SUMO gene from Tomato (Solanum lycopersicum L.), cv Saudi-1, named SlS-SUMO1 gene by PCR using specific primers. This gene has SUMO member's features such as C-terminal diglycine (GG) motif as processing site by ULP (ubiquitin-like SUMO protease) and has SUMO consensus ΨKXE/D sequence. Phylogenetic analysis showed that SlS-SUMO1 gene is highly conserved and homologous to Potatoes Ca-SUMO1 and Ca-SUMO2 genes based on sequence similarity. Expression protein of SlS-SUMO1 gene found to be localized in the nucleus, cytoplasm, and nuclear envelop or nuclear pore complex. SUMO conjugating enzyme SCE1a with SlS-SUMO1 protein co-expressed and co-localized in nucleus and formed nuclear subdomains. This study reported that the SlS-SUMO1 gene is a member of SUMO family and its SUMO protein processing using GG motif and activate and transport to nucleus through Sumoylation system in the plant cell.

Assessment of mutant tomato lines as a starting material for breeding varieties resistant to Alternaria alternata

“…Tomato ( Solanum lycopersium Mill.) is among the most cultivated and consumed vegetable crops around the world, including Russia ( Al-Amri, 2013 , Nekoval et al, 2020a , Salman et al, 2020 ). Krasnodar Krai is one of the leading regions of Russia in terms of area and production of open ground tomato.…”

Molecular Characterization and Functional Localization of a Novel SUMOylation Gene in Oryza sativa

“…The SUMO system contains several protein isoforms which participate in SUMOylation reactions [ 4 ]. SUMOylation is a mechanism that occurs in the nucleus and cytoplasm via a cascade of parallel enzymatic reactions, including activation enzymes (E1-SUMO), conjugation enzymes (E2-SUMO), and ligation enzymes (E3-SUMO) [ 13 , 14 ]. It often occurs in lysine (K) inside the SUMO consensus sequence ΨKXE/D (ψ, a hydrophobic residue; K, lysine; X, any amino acid; and E/D, glutamic, or aspartic) [ 11 , 15 , 16 ].…”

Influence of a biological fungicide on the development and spreading of Alternaria alternata on vegetable crops

“…Tomato (Solanum lycopersium Mill. ), pepper (Capsicum annuum L.) and eggplant (Solanum melongena L.) all over the world, including Russia, are among the most cultivated and consumed vegetable crops [1][2][3][4]. So, for example, Krasnodar Krai is one of the leading regions of Russia in terms of area and production of tomato in open ground.…”