Structural and Functional Characterization of Exopolysaccharide Produced by a Novel Isolate Bacillus sp. EPS003

Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

“…The fact that sugar reducing function in polysaccharides is not available for the DNS reaction [27] and that these compounds are not volatiles could explain why a diminution in sugars is seen via quantification and why it is not detected by GC-MS. These results are supported in the literature by the description of some exopolysaccharides secretions by B. megaterium [24]. Several studies have also described B. megaterium as a producer of extracellular proteins [19,28].…”

“…A total of 75 compounds were found to be present in BC extracts and not present in CS2 extracts, as illustrated in Table 3 for analysis without derivatization, and in Table 4 for analysis with derivatization. Some of these identified compounds were strictly found in BC extracts, suggesting a probable production by B. megaterium: 10 fatty acids derivatives (23 ′ , 32 ′ , 34 ′ , 39 ′ , 42 ′ , 44 ′ , 46 ′ , 47 ′ , 36, 44), 5 linear hydrocarbons (4,12,20,24,25), 5 cyclic hydrocarbons with 3 isomers (5,6,8,9,10,11,15,18), 6 amino acids (10 ′ , 12 ′ , 15 ′ , 18 ′ , 20 ′ , 22 ′ ), 2 dipeptides (29, 47), 2 cyclic dipeptides (46, 64), 6 aromatic hydrocarbons (1,16,26,27,31,33), 2 phthalates (59, 61), 2 sugar acids with 1 isomer (9 ′ , 24 ′ , 41 ′ ), 4 polyols with 1 isomer (2 ′ , 4 ′ , 5 ′ , 6 ′ , 21 ′ ), 2 cyclitols (29 ′ , 43 ′ ) and 17 others were identified (3,7,13,14,19,21,23,32,37,43,45,48,50,62, 63, 17 ′ , 38 ′ ). tr indicates integration inferior to 0.1 × 10 6 ; "ND": not detected.…”

“…In this regard, B. megaterium has already been commercialized for agricultural applications through its inoculation as a living organism [17]. But few B. megaterium metabolites have been described so far (phytohormones [20], antibiotic lipopeptides [21], vitamin B12 [22], siderophores [23], biopolymer [18], carotenoids [23], exopolysaccharides [24]) and valuated through their direct utilization for agriculture industry. In the present work, in order to identify its secondary metabolites related to plant growth promotion, a composition and activity analysis of B. megaterium filtrated supernatant was achieved through extraction, chemical family quantification, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), antioxidant analysis and seed germination.…”

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

“…All DT1-0, DT1-2, and DT1-5 exhibit high water solubility, estimated at 95.4 ± 1.5%, 92.8 ± 0.9%, and 97.1 ± 1.0%, respectively. These values exceed those of EPS003 [ 20 ], EPS produced by Weissella cibaria YB-1 [ 21 ], and EPS-B108. Moreover, highly water-soluble EPS typically exhibit strong hydrophilicity, which makes them well-suited for use in food applications as biologically active surfactants and stabilizers.…”