Structural and Functional Characterization of an Orphan ATP-Binding Cassette ATPase Involved in Manganese Utilization and Tolerance in Leptospira spp

Benaroudj, Nadia; Saul, F.A.; Bellalou, Jacques; Miras, Isabelle; Weber, Patrick; Bondet, Vincent; Murray, Gerald L; Adler, Ben; Ristow, Paula; Louvel, Hélène; Haouz, Ahmed; Picardeau, Mathieu

doi:10.1128/jb.00915-13

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Several in vitro studies were conducted to determine the effects of trace elements on Leptospira spp growth. In one study, the effects of Mn 2+ or Fe 2+ on leptospire growth were examined in wild-type serovars compared to LEPBla2866 mutant Leptospira in which ATP-binding cassette (ABC) ATPase was inactivated [ 76 ]. The wild-type Leptospira grew 100% in media with either Mn 2+ or Fe 2+ , while the LEPBla2866 mutant had a 50% reduction in growth in the medium containing Mn 2+ ( p = 0.05).…”

Section: Resultsmentioning

confidence: 99%

Micronutrients and Leptospirosis: A Review of the Current Evidence

et al. 2016

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BackgroundLeptospirosis is one of the most widespread zoonoses and represents a major threat to human health. Due to the high burden of disease, limitations in diagnostics, and limited coverage and availability of effective human and veterinary vaccines, leptospirosis remains an important neglected zoonotic disease. Improved surveillance and identification of modifiable risk factors for leptospirosis are urgently needed to inform preventive interventions and reduce the risk and severity of Leptospira infection.Methodology/Principal FindingsThis review was conducted to examine the evidence that links micronutrient status and Leptospira infection. A total of 56 studies were included in this review: 28 in vitro, 17 animal, and 11 observational human studies. Findings indicated that Leptospira infection is associated with higher iron and calcium concentrations and hypomagnesemia.Conclusions/SignificanceFew prospective studies and no randomized trials have been conducted to date to examine the potential role of micronutrients in Leptospira infection. The limited literature in this area constrains our ability to make specific recommendations; however, the roles of iron, calcium, and magnesium in leptospirosis represent important areas for future research. The role of micronutrients in leptospirosis risk and severity needs to be elucidated in larger prospective human studies to inform public health interventions.

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Section: Resultsmentioning

confidence: 99%

Micronutrients and Leptospirosis: A Review of the Current Evidence

et al. 2016

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“…Further, significant domain class proteins like chaperone proteins, VOC family, SGNH hydrolase, ComF, and ATP binding cassette proteins were also identified from our study. Previous studies reported the presence of these domains in Leptospira determining the functions like survival under stress conditions, cell-cell signaling, and binding to membrane receptors [ 49 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein in Leptospira interrogans Grown in EMJH Medium

et al. 2021

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Leptospirosis is a re-emerging form of zoonosis that is caused by the spirochete pathogen Leptospira. Extracellular proteins play critical roles in the pathogenicity and survival of this pathogen in the host and environment. Extraction and analysis of extracellular proteins is a difficult task due to the abundance of enrichments like serum and bovine serum albumin in the culture medium, as is distinguishing them from the cellular proteins that may reach the analyte during extraction. In this study, extracellular proteins were separated as secretory proteins from the culture supernatant and surface proteins were separated during the washing of the cell pellet. The proteins identified were sorted based on the proportion of the cellular fractions and the extracellular fractions. The results showed the identification of 56 extracellular proteins, out of which 19 were exclusively extracellular. For those proteins, the difference in quantity with respect to their presence within the cell was found to be up to 1770-fold. Further, bioinformatics analysis elucidated characteristics and functions of the identified proteins. Orthologs of extracellular proteins in various Leptospira species were found to be closely related among different pathogenic forms. In addition to the identification of extracellular proteins, this study put forward a method for the extraction and identification of extracellular proteins.

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Survival Tests for Leptospira spp.

Mouville

Benaroudj

2020

Methods in Molecular Biology

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Measuring viability is an important and necessary assessment in studying microorganisms. Several methods can be applied to Leptospira spp, each with advantages and inconveniencies. Here, we describe the traditional colony-forming unit method, together with two other methods based respectively on the reducing capacity of live cells (Alamar Blue Assay) and differential staining of live and dead cells (LIVE/DEAD BacLight). The Alamar Blue Assay uses the blue reagent resazurin, which can be reduced into the pink reagent resorufin by live cell oxidoreductases. Production of resorufin can be quantified by absorbance or fluorescence reading. The LIVE/DEAD BacLight assay uses a mixture of two nucleic acid dyes (Syto9 and propidium iodide) that differentially penetrate and stain nucleic acid of cells with decreased membrane integrity. The colony-forming unit method is labor-intensive but the most sensitive and linear method. The two other methods are not laborious and well-adapted to high throughput studies but the range of detection and linearity is limited.

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Structural and Functional Characterization of an Orphan ATP-Binding Cassette ATPase Involved in Manganese Utilization and Tolerance in Leptospira spp

Cited by 3 publications

References 47 publications

Micronutrients and Leptospirosis: A Review of the Current Evidence

Micronutrients and Leptospirosis: A Review of the Current Evidence

Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein in Leptospira interrogans Grown in EMJH Medium

Survival Tests for Leptospira spp.

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