Structural and functional analysis of the S proteins of two human coronavirus OC43 strains adapted to growth in different cells

Kunkel, Frank; Herrler, Georg

doi:10.1007/bf01718615

Cited by 18 publications

(21 citation statements)

References 13 publications

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“…3. Alignment of the nucleotide sequences of the 568-nucleotide PCR product from TCV with the corresponding S gene sequences of HCV-OC43 (Kuenkel and Herrler, 1996; accession number Z32768) and BCV-L9 (Zhang et al, 1991;accession number M64667). Nucleotides identical to the consensus sequence are shown as dots.…”

Section: Methods and Resultsmentioning

confidence: 99%

“…A nested-PCR product of 568 bp nucleotide fragment was obtained. The 568-nucleotide sequence analyzed by the computer program Megalign (DNASTAR, Madison, WI) for matching sequences with BCV-L9 (Zhang et al, 1991;accession number M64667) or human coronavirus (HCV-OC43) (Kuenkel and Herrler, 1996; accession number Z32768) revealed high degree of nucleotide sequence homology among them. Pairwise comparisons of the nucleotide sequence to the corresponding S gene sequences of BCV or HCV-OC43 demonstrated 99% of similarity.…”

Section: Methods and Resultsmentioning

confidence: 99%

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Characterization of turkey coronavirus from turkey poults with acute enteritis

Lin

Loa

Tsai

et al. 2002

Veterinary Microbiology

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The present study was to characterize turkey coronavirus associated with turkey poult enteritis and mortality. Intestinal contents or intestines from affected turkey poults and inoculated turkey embryos contained coronaviruses as revealed by electron microscopy or were positive for turkey coronavirus by immunofluorescent antibody assay. Sucrose density gradient ultracentrifugation of the virus-containing intestinal homogenate yielded two opalescent bands corresponding to the buoyant densities of 1.14-1.15 and 1.18-1.20 g/ml, respectively. Coronaviral particles from intestinal contents or the sucrose density gradient preparation were mainly spherical in shape and had envelope and central depression. They were surrounded by a fringe of regularly spaced petal-shaped projections attached to the particles by a short stalk. Purified viruses hemagglutinated rabbit erythrocytes with a titer of 16. Major protein bands of purified viruses analyzed by SDS-PAGE were located at 200, 100-110, 50-60, and 30-35 kDa. The patterns of protein bands were consistent with those of Minnesota or Quebec turkey coronavirus isolates. A 568 bp nucleotide fragment of turkey coronavirus spike protein gene was amplified from RNA of inoculated turkey embryo intestine or purified virus. Sequence analysis of the 568 bp PCR product revealed high degree of identity with the corresponding spike protein gene sequence of human and bovine coronaviruses. The results indicated that turkey coronavirus was associated with turkey poults with acute enteritis.

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Section: Methods and Resultsmentioning

confidence: 99%

Section: Methods and Resultsmentioning

confidence: 99%

Characterization of turkey coronavirus from turkey poults with acute enteritis

Lin

Loa

Tsai

et al. 2002

Veterinary Microbiology

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“…1). In all laboratory isolates of HCoV-OC43, viruses encoding these proteins were passaged at least a few times in tissue culture cells (Kunkel and Herrler, 1993b;Kunkel and Herrler, 1996;Mounir and Talbot, 1993;St-Jean et al, 2004;Vijgen et al, 2005b). While no two sequences are identical, three substitutions, T33S, N491H and R759H, are unique to the HCoV-OC43 NV strain (Table 1 and Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

Murine encephalitis caused by HCoV-OC43, a human coronavirus with broad species specificity, is partly immune-mediated

et al. 2006

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The human coronavirus HCoV-OC43 causes a significant fraction of upper respiratory tract infections. Most coronaviruses show a strong species specificity, although the SARS-Coronavirus crossed species from palm civet cats to infect humans. Similarly, HCoV-OC43, likely a member of the same coronavirus group as SARS-CoV, readily crossed the species barrier as evidenced by its rapid adaptation to the murine brain [McIntosh, K., Becker, W.B., Chanock, R.M., 1967. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc Natl Acad Sci U.S.A. 58, 2268-73]. Herein, we investigated two consequences of this plasticity in species tropism. First, we showed that HCoV-OC43 was able to infect cells from a large number of mammalian species. Second, we showed that virus that was passed exclusively in suckling mouse brains was highly virulent and caused a uniformly fatal encephalitis in adult mice. The surface glycoprotein is a major virulence factor in most coronavirus infections. We identified three changes in the HCoV-OC43 surface glycoprotein that correlated with enhanced neurovirulence in mice; these were located in the domain of the protein responsible for binding to host cells. These data suggest that some coronaviruses, including HCoV-OC43 and SARS-CoV, readily adapt to growth in cells from heterologous species. This adaptability has facilitated the isolation of HCoV-OC43 viral variants with markedly differing abilities to infect animals and tissue culture cells.

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“…In experimental studies, when human cell cultures are pretreated with monoclonal antibodies against hAPN, viral infection appears blocked [48]. However, HCoV-OC43 gains entry into the respiratory epithelium by using the two surface glycoproteins, Haemagglutinin-esterase (HE) and Spike (S) glycoproteins [49]. Despite their differences in pathogenesis, they both cause similar clinical manifestations [50].…”

Section: Pathogenesismentioning

confidence: 99%

The common cold: a review of the literature

Wat

2004

European Journal of Internal Medicine

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Respiratory viral infections, also known as the common cold, are the most common infections in humans. Despite their benign nature, they are a major cause of morbidity and mortality on a worldwide basis. Several viruses have been associated with such illness, of which rhinovirus is the most common. Symptom production is a combination of viral cytopathic effect and the activation of inflammatory pathways. Therefore, antiviral treatment alone may not be able to prevent these events. The optimal use of such agents also requires earlier initiation; therefore, it is important to develop accurate and rapid diagnostic techniques for respiratory viruses. Before any reliable and effective treatment is available, symptomatic therapies may remain the only possible choice of management.

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Structural and functional analysis of the S proteins of two human coronavirus OC43 strains adapted to growth in different cells

Cited by 18 publications

References 13 publications

Characterization of turkey coronavirus from turkey poults with acute enteritis

Characterization of turkey coronavirus from turkey poults with acute enteritis

Murine encephalitis caused by HCoV-OC43, a human coronavirus with broad species specificity, is partly immune-mediated

The common cold: a review of the literature

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