Structural and Expression Analysis of Salinity Stress Responsive Phosphoserine Phosphatase from Brassica juncea (L.)

Purty, Ram Singh; Sachar, Madhav; Chatterjee, Sayan

doi:10.4172/jpb.1000432

Cited by 10 publications

(7 citation statements)

References 47 publications

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“…Phosphoserine phosphatase (PSP) is inhibited by high concentrations of serine ( Ho and Saito, 2001 ). It exhibits 1.5-fold abundance in the root compared with the leaf tissues ( Ho et al, 1999a ) and is induced under salinity stress ( Purty et al, 2017 ). The enzyme was purified from Arabidopsis ( Ho et al, 1999a ), and its similarity to the mammal and bacterial enzyme has been demonstrated ( Basurko et al, 1999 ).…”

Section: Phosphorylated (Phosphohydroxypyruvate) Serine Pathwaymentioning

confidence: 99%

The Glycerate and Phosphorylated Pathways of Serine Synthesis in Plants: The Branches of Plant Glycolysis Linking Carbon and Nitrogen Metabolism

Igamberdiev

Kleczkowski

2018

Front. Plant Sci.

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Serine metabolism in plants has been studied mostly in relation to photorespiration where serine is formed from two molecules of glycine. However, two other pathways of serine formation operate in plants and represent the branches of glycolysis diverging at the level of 3-phosphoglyceric acid. One branch (the glycerate – serine pathway) is initiated in the cytosol and involves glycerate formation from 3-phosphoglycerate, while the other (the phosphorylated serine pathway) operates in plastids and forms phosphohydroxypyruvate as an intermediate. Serine formed in these pathways becomes a precursor of glycine, formate and glycolate accumulating in stress conditions. The pathways can be linked to GABA shunt via transamination reactions and via participation of the same reductase for both glyoxylate and succinic semialdehyde. In this review paper we present a hypothesis of the regulation of redox balance in stressed plant cells via participation of the reactions associated with glycerate and phosphorylated serine pathways. We consider these pathways as important processes linking carbon and nitrogen metabolism and maintaining cellular redox and energy levels in stress conditions.

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Section: Phosphorylated (Phosphohydroxypyruvate) Serine Pathwaymentioning

confidence: 99%

The Glycerate and Phosphorylated Pathways of Serine Synthesis in Plants: The Branches of Plant Glycolysis Linking Carbon and Nitrogen Metabolism

Igamberdiev

Kleczkowski

2018

Front. Plant Sci.

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“…LOC_Os05g09724 , encoding HAD superfamily phosphatase, is the only annotated gene in the green module that was detected by DG and CN. One of the Haloacid Dehalogenase (HAD) superfamily, phosphoserine phosphatase from Brassica juncea L, was upregulated in salt stress ( Purty et al, 2017 ) and the HAD1 gene in soybean ( GmHAD1 ) was shown to be involved in low phosphorus stress tolerance ( Cai et al, 2018 ). In saline soil, P solubility is decreased due to high Na + concentration and soil pH ( Martinez et al, 1996 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Key Genes in ‘Luang Pratahn’, Thai Salt-Tolerant Rice, Based on Time-Course Data and Weighted Co-expression Networks

et al. 2021

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Salinity is an important environmental factor causing a negative effect on rice production. To prevent salinity effects on rice yields, genetic diversity concerning salt tolerance must be evaluated. In this study, we investigated the salinity responses of rice (Oryza sativa) to determine the critical genes. The transcriptomes of ‘Luang Pratahn’ rice, a local Thai rice variety with high salt tolerance, were used as a model for analyzing and identifying the key genes responsible for salt-stress tolerance. Based on 3' Tag-Seq data from the time course of salt-stress treatment, weighted gene co-expression network analysis was used to identify key genes in gene modules. We obtained 1,386 significantly differentially expressed genes in eight modules. Among them, six modules indicated a significant correlation within 6, 12, or 48h after salt stress. Functional and pathway enrichment analysis was performed on the co-expressed genes of interesting modules to reveal which genes were mainly enriched within important functions for salt-stress responses. To identify the key genes in salt-stress responses, we considered the two-state co-expression networks, normal growth conditions, and salt stress to investigate which genes were less important in a normal situation but gained more impact under stress. We identified key genes for the response to biotic and abiotic stimuli and tolerance to salt stress. Thus, these novel genes may play important roles in salinity tolerance and serve as potential biomarkers to improve salt tolerance cultivars.

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“…Upon exposure to abiotic stress several genes/proteins gets up- or down regulated [ [39] , [40] , [41] ]. These proteins are believed to play vital role against biotic and abiotic stresses in plants.…”

Section: Discussionmentioning

confidence: 99%

In silico identification and validation of miRNA and their DIR specific targets in Oryza sativa Indica under abiotic stress

Singh

Mehra

Chatterjee

et al. 2020

Non-coding RNA Research

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Several biotic (bacterial and viral pathogenesis) and abiotic stress factors like salt, drought, cold, and extreme temperatures significantly reduce crop productivity and grain quality throughout the world. MicroRNAs (miRNAs) are small (~22 nucleotides) non-coding endogenous RNA molecules which negatively regulate gene expression at the post-transcriptional level either by degrading the target protein-coding mRNA genes or suppressing translation in plants. Dirigent (DIR) gene protein plays a crucial role as they are involved to dictate the stereochemistry of a compound synthesized by other enzymes as well as in lignifications against biotic and abiotic stress. In plants, several miRNAs, as well as their targets, are known to regulate stress response but systematic identification of the same is limited. The present work has been designed for in silico identification of miRNAs against a total of sixty-one DIR genes in Oryza sativa Indica followed by target prediction of identified miRNAs through the computational approach and thereafter validation of potential miRNAs in rice genotypes. We systematically identified 3 miRNA and their respective DIR specific target gene in Oryza sativa Indica. The expression of these three miRNAs and their respective DIR specific targets were validated in rice seedlings subjected to five different abiotic stress conditions (heavy metal, high temperature, low temperature, salinity and drought) by quantitative Real-Time PCR (qRT-PCR). Expression analysis indicated that miRNA under stress conditions regulates the gene expression of the DIR gene in rice. To the best of our knowledge this is this is the first report in any organism showing the expression of ath-miRf10317-akr, and osamiRf10761-akr miRNAs in response to various abiotic stresses.

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Structural and Expression Analysis of Salinity Stress Responsive Phosphoserine Phosphatase from Brassica juncea (L.)

Cited by 10 publications

References 47 publications

The Glycerate and Phosphorylated Pathways of Serine Synthesis in Plants: The Branches of Plant Glycolysis Linking Carbon and Nitrogen Metabolism

The Glycerate and Phosphorylated Pathways of Serine Synthesis in Plants: The Branches of Plant Glycolysis Linking Carbon and Nitrogen Metabolism

Identification of Key Genes in ‘Luang Pratahn’, Thai Salt-Tolerant Rice, Based on Time-Course Data and Weighted Co-expression Networks

In silico identification and validation of miRNA and their DIR specific targets in Oryza sativa Indica under abiotic stress

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