Structural and developmental analysis of two linked myosin heavy chain genes

Parker-Thornburg, Janice; Bauer, Beverly Jean; Palermo, Joseph; Robbins, Jeffrey

doi:10.1016/0012-1606(92)90010-e

Cited by 28 publications

(21 citation statements)

References 35 publications

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“…This represents a striking example of uniform gene expression and uniform fiber-type composition. Conversely, neither TnC-slow gene transcripts (this study) nor type I/b MHC (Parker- Thornburg et al, 1992) are detected in the tongue after birth. Type I/b-MHC is also not detectable at day E17 of fetal development in the tongue (Parker-Thornburg et al, 1992).…”

Section: Discussioncontrasting

confidence: 58%

“…Conversely, neither TnC-slow gene transcripts (this study) nor type I/b MHC (Parker- Thornburg et al, 1992) are detected in the tongue after birth. Type I/b-MHC is also not detectable at day E17 of fetal development in the tongue (Parker-Thornburg et al, 1992). However, using the more sensitive RT-PCR method, we did detect slow/type I/b-MHC at E17 as well as E18, although our immunofluorescent labeling using the NCL-MHCs antibody demonstrated that type I/b MHC was present in only a small subpopulation of fibers (Fig.…”

Section: Discussioncontrasting

confidence: 58%

“…The homology of MHC genes permitted few regions for amplification to detect specific isoforms. We were able to amplify the transcript derived from the mdms gene (Parker- Thornburg et al, 1992) (Fig. 6A), which has been shown to correspond to a protein isoform type IIX (DeNardi et al, 1993).…”

Section: Uniform Mhc Protein Content Of the Tongue Is Regulated At Thmentioning

confidence: 99%

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Differential expression of troponin C genes during tongue myogenesis

et al. 1997

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Determination of muscle fiber type is related to the developmental stage of the tissue. Ordinarily the final distribution of fast and slow fibers in a muscle is determined postnatally. Tongue muscle, however, is composed solely of fast-twitch fibers that express only troponin C fast mRNA and fast (type II) myosin heavy chain (MHC) proteins in both the adult and the one-dayold mouse. The fiber-type determination of this muscle was examined during fetal development. Both troponin C fast and slow mRNAs were expressed at initial stages of tongue development at embryonic day 13. However, by embryonic day 16 the troponin C fast transcripts predominated. At 17 days of embryonic development, TnC fast mRNA was 10 times more abundant than TnC slow, and at 18 days of development the TnC slow mRNA was barely detectable. The tongue muscle myotubes expressed fast, slow, and embryonic MHC isoforms during early embryonic development. At 18 days of gestation, the MHC isoform expressed by the majority of the myotubes was the fast isoform, whereas the slow isoform was present in very few fibers. RT-PCR analysis of the MHC transcripts present throughout tongue development demonstrated expression of the mdms or type IIx MHC in both late fetal and postnatal stages of development. In contrast, the type I/b slow MHC mRNA was undetectable in the postnatal and adult tongue. The absence of TnC and MHC slow-isoform mRNAs in the newborn mouse tongue suggests that slow isoform genes become dominantly repressed with the TnC-F and MHC type IIx genes remaining transcriptionally active, giving rise to an unusually homogeneous fast-twitch phenotype. The tongue muscle fibers acquire their specific adult-type fiber characteristics during fetal development rather than postnatally. Dev. Dyn. 209:36-44, 1997. r 1997 Wiley-Liss, Inc.

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Section: Discussioncontrasting

confidence: 58%

Section: Discussioncontrasting

confidence: 58%

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Differential expression of troponin C genes during tongue myogenesis

et al. 1997

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“…Thus, cis-elements in the proximal promoter of the slow MyHC 3 gene appear to be sufficient to direct expression to slow embryonic muscle fibers in vitro. DISCUSSION A family of myosin heavy chain genes have been identified in mammalian (7,8) and avian (9 -11) genomes. The expression of different members of this family has been shown to be regulated in developmental stage-specific (5, 21), fiber type-specific (6,53), and hormone-dependent (27) fashion.…”

Section: Figmentioning

confidence: 99%

“…Numerous MyHC isoforms of the fast class have been identified in both mammalian and avian striated muscles, and genes encoding many of the fast MyHC isoforms have been isolated (7)(8)(9)(10)(11). In mammals, the only MyHC isoform characterized in slow skeletal muscle is encoded by the ␤-cardiac/slow MyHC gene (12)(13)(14)(15), although immunological data suggests that additional slow isoforms may exist (16).…”

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confidence: 99%