Structural and biochemical correlates of Na+,K+‐ATPase driven ion uptake across the posterior gill epithelium of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae)

Furriel, Rosa dos Prazeres Melo; Firmino, Kelly Cristina Silva; Masui, Douglas Chodi; Faleiros, Rogério Oliveira; Torres, Antonio Hernandes; McNamara, John Campbell

doi:10.1002/jez.622

Cited by 45 publications

(54 citation statements)

References 69 publications

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“…olfersi , M. rosenbergii (FRANÇA et al, 2013), Clibanarius vittatus (GONÇALVES et al, 2006;LUCENA et al, 2012), Callinectes ornatus (GARÇON et al, 2007; (FURRIEL et al, 2001). …”

Section: Figura 3 Esquema Da Estrutura Das Subunidades Da (Na + K +mentioning

confidence: 99%

“…Vários pesquisadores tem focado seus esforços no estudo da capacidade da (Na + , K + )-ATPase em hidrolisar o p-nitrofenilfosfato (PNFF) (GACHE et al, 1977;GLYNN, 1985;BERBERIAN & BEAUGÉ, 1992;SPECHT et al, 1997;FURRIEL et al, 2001;HOMAREDA & USHIMARU, 2005). Entretanto, existem controvérsias a respeito do mecanismo de reação da enzima quando esse substrato não fisiológico é utilizado.…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…O uso de p-nitrofenilfosfato como substrato para caracterizar cineticamente a atividade de K + -fosfatase da enzima das brânquias de crustáceos provou ser uma alternativa conveniente para estudos comparativos da atividade (Na + ,K + )-ATPase não só em M. olfersi (FURRIEL et al , 2001, MENDONÇA et al, 2007, mas também no caranguejo Portunidae, C. danae (MASUI et al, 2003;MASUI et al, 2005b), no camarão M. amazonicum (BELLI et al, 2009), e no ermitão Clibanarius symmetricus . A caracterização cinética da atividade K + -fosfatase em M. amazonicum juvenil e adulto ajudou a elucidar a capacidade osmoregulatória nestes dois estágios do ciclo de vida (LEONE et al, 2013 (FURRIEL et al, 2001MASUI et al, 2005;MENDONÇA et al, 2007;BELLI et al, 2009;GARÇON et al, 2012;GARÇON et al, 2013;LEONE et al, 2013).…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…A caracterização cinética da atividade K + -fosfatase em M. amazonicum juvenil e adulto ajudou a elucidar a capacidade osmoregulatória nestes dois estágios do ciclo de vida (LEONE et al, 2013 (FURRIEL et al, 2001MASUI et al, 2005;MENDONÇA et al, 2007;BELLI et al, 2009;GARÇON et al, 2012;GARÇON et al, 2013;LEONE et al, 2013). O valor de atividade máxima observado para C. guanhumi foi menor quando comparados aos camarões M. amazonicum e M. olfersii, ao siri C. danae e ao ermitão Clibanarius vittatus (BELLI et al, 2009;LEONE et al, 2012;FURRIEL et al, 2001;MASUI et al, 2003;GARÇON et al, 2012).…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…O valor da afinidade aparente da enzima de C. guanhumi para o p-nitrofenilfosfato (K 0.5 = 2,9 ± 0,14 mmol L -1 ) é semelhante ao do siri C. danae recém-capturado (MASUI et al, 2003), do camarão M. olfersi em água doce (FURRIEL et al, 2001), e aclimatado à 21‰…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

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Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Farı́as¹

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“…olfersi , M. rosenbergii (FRANÇA et al, 2013), Clibanarius vittatus (GONÇALVES et al, 2006;LUCENA et al, 2012), Callinectes ornatus (GARÇON et al, 2007; (FURRIEL et al, 2001). …”

Section: Figura 3 Esquema Da Estrutura Das Subunidades Da (Na + K +mentioning

confidence: 99%

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

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Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Farı́as¹

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Low salinity‐induced alterations in epithelial ultrastructure, Na⁺/K⁺‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab, Clibanarius vittatus (Anomura, Diogenidae)

et al. 2017

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Fresh caught Clibanarius vittatus [SW, 31‰ salinity (S)] were acclimated to a dilute medium (15‰ S) for 10 days, employing silver staining to locate gill ion transporting tissue, immunofluorescence to localize the Na/K-ATPase α-subunit in the lamellae, and electron microscopy to portray ultrastructural changes in the gill epithelia. Na/K-ATPase activity was characterized kinetically in a gill microsomal fraction, including synergistic stimulation by NH plus K. Silver staining revealed that all 26 phyllobranchiate arthro- and pleurobranchiae participate in ion transport. Na/K-ATPase α-subunit staining was weak in SW crabs and distributed exclusively and irregularly within the intralamellar septal cells, particularly at the septal-pillar cell body junctions, and septal cell cytoplasm facing the hemolymph space. In 15‰ S crabs, α-subunit localization was intense, occupying the entire thickened septum. Pillar cells and flanges did not stain. Mitochondria and membrane foldings increased in the pillar cell flanges and intralamellar septal cells, greatly amplifying surface area. Only a single ATP binding site (V = 130.8 ± 10.5 nmol min mg protein; K = 55.3 ± 1.7 μmol l) obeying Michaelis-Menten kinetics was disclosed. Na/K-ATPase activity was modulated by Mg, Na, and NH, exhibiting site-site interactions; K modulation showed Michaelis-Menten kinetics. K plus NH synergistically stimulated activity ≈ 1.7-fold. Ouabain inhibited total ATPase activity by ≈ 70% (K = 220-300 μmol l), revealing phosphohydrolytic activities other than the Na/K-ATPase. Despite ample phylogenetic separation, the phyllobranchiate lamellae of the Anomura and Caridea share many ultrastructural features, that is, an intralamellar septum and opposed abutting pillar cells, similar Na/K-ATPase distribution, and comparable kinetic characteristics. These findings suggest either convergent evolution at the structural and biochemical levels, or preservation of traits present in a remote common ancestor.

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A systematic evaluation on the relationship between hypo‐osmoregulation and hyper‐osmoregulation in decapods of different habitats

Bozza,

Freire,

Prodocimo

2023

J Exp Zool Pt A

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Decapods occupy all aquatic, and terrestrial and semi‐terrestrial environments. According to their osmoregulatory capacity, they can be osmoconformers or osmoregulators (hypo or hyperegulators). The goal of this study is to gather data available in the literature for aquatic decapods and verify if the rare hyporegulatory capacity of decapods is associated with hyper‐regulatory capacity. The metric used to quantify osmoregulation was the osmotic capacity (OC), the gradient between external and internal (hemolymph) osmolalities. We employ phylogenetic comparative methods using 83 species of decapods to test the correlation between hyper OC and hypo OC, beyond the ancestral state for osmolality habitat, which was used to reconstruct the colonization route. Our analysis showed a phylogenetic signal for habitat osmolality, hyper OC and hypo OC, suggesting that hyper‐hyporegulators decapods occupy similar habitats and show similar hyper and hyporegulatory capacities. Our findings reveal that all hyper‐hyporegulators decapods (mainly shrimps and crabs) originated in estuarine waters. Hyper OC and hypo OC are correlated in decapods, suggesting correlated evolution. The analysis showed that species which inhabit environments with intense salinity variation such as estuaries, supratidal and mangrove habitats, all undergo selective pressure to acquire efficient hyper‐hyporegulatory mechanisms, aided by low permeabilities. Therefore, hyporegulation can be observed in any colonization route that passes through environments with extreme variations in salinity, such as estuaries or brackish water.

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Structural and biochemical correlates of Na⁺,K⁺‐ATPase driven ion uptake across the posterior gill epithelium of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae)

Cited by 45 publications

References 69 publications

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Low salinity‐induced alterations in epithelial ultrastructure, Na⁺/K⁺‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab, Clibanarius vittatus (Anomura, Diogenidae)

A systematic evaluation on the relationship between hypo‐osmoregulation and hyper‐osmoregulation in decapods of different habitats

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Structural and biochemical correlates of Na+,K+‐ATPase driven ion uptake across the posterior gill epithelium of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae)

Cited by 45 publications

References 69 publications

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Low salinity‐induced alterations in epithelial ultrastructure, Na+/K+‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab, Clibanarius vittatus (Anomura, Diogenidae)

A systematic evaluation on the relationship between hypo‐osmoregulation and hyper‐osmoregulation in decapods of different habitats

Contact Info

Product

Resources

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Structural and biochemical correlates of Na⁺,K⁺‐ATPase driven ion uptake across the posterior gill epithelium of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae)

Low salinity‐induced alterations in epithelial ultrastructure, Na⁺/K⁺‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab, Clibanarius vittatus (Anomura, Diogenidae)