Structural analysis of the curdlan-like exopolysaccharide produced by Cellulomonas flavigena KU

Kenyon, William J.; Buller, C.

doi:10.1038/sj.jim.7000277

Cited by 32 publications

(11 citation statements)

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“…[25] the gram-positive bacteria Cellulomonas flavigena [26] and the sclerotia of the basidiomycete fungus Poria cocos [27]. In S. cerevisiae , (1→3),(1→6)-β-glucan, which comprises approximately 50% of the cell wall, forms a core where the non-reducing termini are covalently linked either to chitin, (1→6)-β-glucan or mannoprotein, which together compose approximately 40% of the wall.…”

Section: Resultsmentioning

confidence: 99%

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Medeiros

Cordeiro²,

Cavalcanti

et al. 2012

IJMS

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Water-insoluble glucan was isolated from the baker’s yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing.

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Section: Resultsmentioning

confidence: 99%

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Medeiros

Cordeiro²,

Cavalcanti

et al. 2012

IJMS

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“…Readings were obtained at room temperature using the sodium D-line and a path length of 1 dm (Kenyon and Buller 2002). …”

Section: Methodsmentioning

confidence: 99%

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

2012

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We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of curdlan in 120 h with a yield of (0.88) while, the wild strain produced 41 g/L in 120 h with a yield of (0.62) in a stirred bioreactor. The mutant could not produce curdlan when the pH was shifted from 7.0 to 5.5 after nitrogen depletion as followed for wild strain. In contrast, pH optimum for cell growth and curdlan production for mutant was found to be 7.0. We optimized the downstream processing of curdlan by varying different volumes of NaOH and HCl for extraction and precipitation of curdlan. The molecular weight of the purified curdlan from the wild and mutant strain was 6.6 × 105 Da and 5.8 × 105 Da respectively. The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units. From the NMR and FTIR data, it has been confirmed that curdlan was exclusively composed of β (1 → 3)-D-glucan residues.

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“…Different buffers such as phosphate -citrate (pH 3 to 5), sodium -phosphate (pH 6 and 7) and HEPES (pH to 7.4 to 9) were used at 50 mM. For determining optimal temperature, standard reactions (100 µl) having 10 µl enzyme (1 mg/ml) and 10 µl of 1% suitable substrate were performed using optimized pH at different temperatures (22,30,37,45, and 54°C) for 2 h.…”

Section: Evaluating Biochemical Properties Of Enzymesmentioning

confidence: 99%

“… 15–18 Curdlan is one of the abundant bio-resources that can be synthesized by several bacteria, including Agrobacterium , 19 Rhizobium , 20 and Cellulomonas species. 21 , 22 In addition, huge quantities of laminarin can accumulate in marine environment upon degradation of macroalgae, and plays a major role in marine carbon cycle. 23 , 24 Therefore, extraction of curdlan and laminarin is much simpler as compared to other natural glycans and can be easily exploited for nutraceutical perspectives.…”

Section: Introductionmentioning

confidence: 99%

Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288^T and their roles in cooperation with gut bacteria

et al. 2020

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β-glucans are the dietary nutrients present in oats, barley, algae, and mushrooms. The macromolecules are well known for their immune-modulatory activity; however, how the human gut bacteria digest them is vaguely understood. In this study, Bacteroides uniformis JCM 13288 T was found to grow on laminarin, pustulan, and porphyran. We sequenced the genome of the strain, which was about 5.05 megabase pairs and contained 4868 protein-coding genes. On the basis of growth patterns of the bacterium, two putative polysaccharide utilization loci for β-glucans were identified from the genome, and associated four putative genes were cloned, expressed, purified, and characterized. Three glycoside hydrolases (GHs) that were endo-acting enzymes (BuGH16, BuGH30, and BuGH158), and one which was an exo-acting (BuGH3) enzyme. The BuGH3, BuGH16, and BuGH158 can cleave linear exo/endo-β-1-3 linkages while BuGH30 can digest endo-β-1-6 linkages. BuGH30 and BuGH158 were further explored for their roles in digesting β-glucans and generation of oligosaccharides, respectively. The BuGH30 predominately found to cleave long chain β-1-6 linked glucans, and obtained final product was gentiobiose. The BuGH158 used for producing oligosaccharides varying from degree of polymerization 2 to 7 from soluble curdlan. We demonstrated that these oligosaccharides can be utilized by gut bacteria, which either did not grow or poorly grew on laminarin. Thus, B. uniformis JCM 13288 T is not only capable of utilizing β-glucans but also shares these glycans with human gut bacteria for potentially maintaining the gut microbial homeostasis.

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Structural analysis of the curdlan-like exopolysaccharide produced by Cellulomonas flavigena KU

Cited by 32 publications

References 0 publications

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288^T and their roles in cooperation with gut bacteria

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Structural analysis of the curdlan-like exopolysaccharide produced by Cellulomonas flavigena KU

Cited by 32 publications

References 0 publications

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750

Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288T and their roles in cooperation with gut bacteria

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Product

Resources

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Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288^T and their roles in cooperation with gut bacteria