Structural analysis of monoterpene glycosides extracted from Paeonia lactiflora Pall. using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

Abstract

Paeoniflorin standard was first investigated by electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) using a sustained off-resonance irradiation (SORI) collision-induced dissociation (CID) method at high mass resolution. The experimental results demonstrated that the unambiguous elemental composition of product ions can be obtained at high mass resolution. Comparing MS/MS spectra and the experimental methods of hydrogen and deuterium exchange, the logical fragmentation pathways of paeoniflorin have been proposed. Then, the extracts of the traditional Chinese medicine Paeonia lactiflora Pall. were analyzed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS). By comparison with the ESI-FTICR-MS/MS data of paeoniflorin, the isomers paeoniflorin and albiflorin in Paeonia lactiflora Pall. have been identified using HPLC/MS with CID in an ion trap and in-source CID. Furthermore, using the characteristic fragmentation pathways, the retention times (t(R)) in HPLC and MS/MS spectra, the structures of three other kinds of monoterpene glycoside compounds have been identified on-line without time-consuming isolation. Thus an HPLC/ESI-MS method for the analysis of constituents in Paeonia lactiflora Pall. has been established.

Systematic chemical profiling of a multicomponent Chinese herbal formula Huo Luo Xiao Ling Dan by ultra high performance liquid chromatography coupled with electrospray ionization quadrupoletime‐of‐flight mass spectrometry

“…S3). In negative mode, paeoniflorin (peak 14) showed a product ion at m/z 449 resulting from the loss of formaldehyde, which is consistent with the reference compound and literature values . The ion at m/z 327 derived from lose of benzoic acid and formaldehyde.…”

NaCl Induces Flavonoid Biosynthesis through a Putative Novel Pathway in Post-harvest Ginkgo Leaves

“…The mass spectra analysis of flavonoids were carried out using a LCQ ion trap mass spectrometer (Finnigan MAT, San Jose, CA, United States) equipped with an ESI as described before (Dong et al, 2007). The mass spectrometer was connected to the HPLC system via an UV cell outlet.…”

Chemical constituents isolated fromPaeonia lactifloraroots and their neuroprotective activity against oxidative stress in vitro

“…lactiflora has been used to treat wounds, fungal infection, pain and dementia in some Asian countries including China and Korea [3]. Monoterpenes, triterpenes and flavonoids have been reported to be isolated from the roots of Paeonia species [4,5].…”