Chemical constituents isolated from<i>Paeonia lactiflora</i>roots and their neuroprotective activity against oxidative stress in vitro

Kim, Seung Hyun; Lee, Mi Kyeong; Lee, Ki Yong; Sung, Sang Hyun; Kim, Jinwoong; Kim, Young Choong

doi:10.1080/14756360802667977

Cited by 58 publications

(39 citation statements)

References 10 publications

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“…Among them, the ethyl acetate fraction of Paeonia lactiflora (EA-PL) presented the highest potency in scavenging DPPH radical when used in combination with four solvent-extracted fractions from AME ( P <0.05), suggesting that EA-PL was rich in DPPH radical-scavenging activity (Fig.1A). Our results were in agreement with the previous report [19]. Herein, CIs were used to determine the possible interactive actions between the extracts or fractions.…”

Section: Resultssupporting

confidence: 93%

In Vitro Synergistic Antioxidant Activity and Identification of Antioxidant Components from Astragalus membranaceus and Paeonia lactiflora

Zhang

et al. 2014

PLoS ONE

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Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membranaceus (AME) and Paeonia Lactiflora (PL), and identify the potential antioxidant components by 1,1-diphenyl-2-picrylhydrazine (DPPH) radical spiking test followed by a high performance liquid chromatography separation combined with diode array detection and tandem mass spectrometry analysis (DPPH-HPLC-DAD-MS/MS). Eight AME-PL combined extracts (E1–E8) were prepared based on bioactivity-guided fractionation. Among them, E1 exhibited the strongest synergistic effect in scavenging DPPH radicals and reducing ferric ions (P<0.05). Moreover, E1 presented strong cytoprotection against H2O2-induced oxidative damage in MRC-5 cells by suppressing the decrease of the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities. A strong correlation between the increment of total phenolic/flavonoid and synergistic antioxidant activity, especially between the increment of total flavonoid and the increase in ferric reducing power was observed. Finally, seven antioxidant substances were identified in E1 as oxypaeoniflora, catechin, calycosin-7-O-β-D-glucopyranoside, fomononetin-7-O-β-D-glucopyranoside, 9,10-dimethoxy-pterocarpan-3-O-β-D-glucopyranoside, quercetin and 2′-dihydroxy-3′,4′-dimethyl-isoflavan-7-O-β-D-glucopyranoside.

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Section: Resultssupporting

confidence: 93%

In Vitro Synergistic Antioxidant Activity and Identification of Antioxidant Components from Astragalus membranaceus and Paeonia lactiflora

Zhang

et al. 2014

PLoS ONE

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“…PF, the major monoterpene glycoside responsible for the biological activity of Paeonia lactiflora Pallas, has a wide range of pharmacological functions, including neuroprotective effects (Kim et al, 2009). Nam and colleagues (2013) have found that PF can suppress LPS-induced overproduction of pro-inflammatory mediators from microglial cells (Nam et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin attenuates Aβ1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-κB- and VEGF/Flt-1 signaling pathways

Liu¹,

Wang

et al. 2015

Brain Research

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“…Kim et al (2009) found that TGP significantly protected primary cultures of rat cortical cells exposed to oxidative stress induced by H 2 O 2 . Paeoniflorin was reported to exert a neuroprotective effect on glutamate-induced neurotoxicity in PC12 cells, via inhibiting oxidative stress and Ca 2+ overload (Mao et al, 2010).…”

Section: Antioxidant Effect Of Tgpmentioning

confidence: 99%

Anti-Inflammatory and Immunomodulatory Effects of Paeonia Lactiflora Pall., a Traditional Chinese Herbal Medicine

2011

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In China, Korea, and Japan, a decoction of the dried root without bark of Paeonia lactiflora Pall. has been used in the treatment of rheumatoid arthritis, systemic lupus erythematosus, hepatitis, dysmenorrhea, muscle cramping and spasms, and fever for more than 1200 years. A water/ethanol extract of the root is now known as total glucosides of peony (TGP), which contains more than 15 components. Paeoniflorin is the most abundant ingredient and accounts for the pharmacological effects observed with TGP in both in vitro and in vivo studies. The analgesic effect of TGP was confirmed in various animal models of pain, which may be mediated partly by adenosine A1 receptor. The direct anti-inflammatory effects of TGP were observed in animal models of both acute and subacute inflammation, by inhibiting the production of prostaglandin E2, leukotriene B4, and nitric oxide, and by suppressing the increase of intracellular calcium ion concentration. TGP was also reported to have protective effects of cells against oxidative stress. In vitro, dual effects of TGP were noted on the proliferation of lymphocytes, differentiation of Th/Ts lymphocytes, and the production of proinflammatory cytokines and antibodies. In vivo, TGP inhibited the delayed-type hypersensitivity in immuno-activated mice, and enhanced the delayed-type hypersensitivity in immuno-suppressed mice. In adjuvant arthritis rats, paeoniflorin exerted immunosuppressive effects. The beneficial effects of TGP in treating rheumatoid arthritis were verified by randomized controlled trials. The adverse events of TGP were mainly gastrointestinal tract disturbances, mostly mild diarrhea.

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Chemical constituents isolated fromPaeonia lactifloraroots and their neuroprotective activity against oxidative stress in vitro

Cited by 58 publications

References 10 publications

In Vitro Synergistic Antioxidant Activity and Identification of Antioxidant Components from Astragalus membranaceus and Paeonia lactiflora

In Vitro Synergistic Antioxidant Activity and Identification of Antioxidant Components from Astragalus membranaceus and Paeonia lactiflora

Paeoniflorin attenuates Aβ1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-κB- and VEGF/Flt-1 signaling pathways

Anti-Inflammatory and Immunomodulatory Effects of Paeonia Lactiflora Pall., a Traditional Chinese Herbal Medicine

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