Structural analysis of fertilization in the fish <i>Brycon orbignyanus</i>

Ganeco, Luciana Nakaghi; Franceschini-Vicentini, Irene Bastos; Nakaghi, Laura Satiko Okada

doi:10.1017/s0967199408005030

Cited by 17 publications

(30 citation statements)

References 29 publications

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“…The micropyle is a small opening in the zona radiata, composed of the vestibule and micropylar channel that reaches the oocyte's plasmatic membrane and participates in the fertilization process (Ganeco and Nakaghi, ). In this study, oocytes of both species showed a funnel‐shaped micropyle, a common pattern similar to other Characiformes species (Ganeco et al., ; Alexandre et al., ; Isaú et al., ).…”

Section: Discussionsupporting

confidence: 86%

Comparative Analysis of the Oocytes and Early Development of Two Species of Curimatidae Teleost Fish

Perini

Sato²,

Rizzo

et al. 2012

Anat Histol Embryol

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Curimatella lepidura and Steindachnerina elegans are small forage fish, constituting an important link in the food chain, serving as food for larger commercial fish. In this study, characteristics of the eggs, of the oocyte's surface ultrastructure and of the embryogenesis are first described for these species. Absolute fecundity was 40864 ± 8769 oocytes for C. lepidura and 22089 ± 8710 oocytes for S. elegans. Oocytes of both species are yellowish, weakly adhesive and with a post-fertilization diameter of 1019.5 ± 20.6 μm and 978.75 ± 29.16 μm for C. lepidura and S. elegans, respectively. The ultrastructural analysis, using scanning electron microscopy, showed that the oocyte's surface of both species has pore canals over the entire surface and a funnel-shaped micropyle. At 24°C, the embryonic development of C. lepidura was completed 25 h after fertilization, and blastopore closure occurred in 7 h 30 min. In S. elegans, larvae hatched 20 h after fertilization, and blastopore closure occurred in 7 h 15 min. The fertilization rate was 74.5 ± 7.96 and 71.2 ± 10.8% for C. lepidura and S. elegans, respectively. This study provides important support for clarifying phylogenetic relationships and in ecological and zoological understanding of Neotropical Curimatidae fish.

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Section: Discussionsupporting

confidence: 86%

Comparative Analysis of the Oocytes and Early Development of Two Species of Curimatidae Teleost Fish

Perini

Sato²,

Rizzo

et al. 2012

Anat Histol Embryol

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“…The diameter of oocytes of B. gouldingi was small and typical of migratory species that exhibit spawning and external fertilization with no parental care (Vazzoler, ). The diameters recorded in this study were similar to those reported in congeneric species such as matrinxã Brycon cephalus (Günther 1869) (Romagosa et al ., ) and piracanjuba Brycon orbignyanus (Valenciennes 1850) (Ganeco et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Morphological and morphometric aspects of early life stages of piabanhaBrycon gouldingi(Characidae)

Faustino

Makino

Neumann³

et al. 2015

Journal of Fish Biology

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Adult specimens of piabanha Brycon gouldingi were collected from Rio das Mortes (Mato Grosso, Brazil), adapted to captivity and induced to spawn at Buriti Fisheries (Nova Mutum, MT, Brazil). The early developmental stages of B. gouldingi were then characterized. Samples were collected at pre-determined times from oocyte extrusion to total yolk absorption. Oocyte diameter, total larval length (LT ) and yolk-sac volume were measured. The mean ± s.d. duration of embryo developmental of B. gouldingi was 13·90 ± 0·06 h at 26·40 ± 1·13° C. The mean ± s.d. oocyte diameter was 1·13 ± 0·06 mm with 54% of oocytes ranging from 1·11 to 1·20 mm. Seven stages characterized the early developmental phase of this species: zygote, cleavage, morula, blastula, gastrula, histogenesis-organogenesis and hatching, with unique features related to each stage. At hatching, the larvae measured 3·40 ± 0·07 mm, presented an elongated shape with yolk-sac volume of 0·46 ± 0·08 µl, non-pigmented eyes and exhibited swimming ability. When the yolk was completely absorbed at 55 h post-hatch, mean ± larval LT was 6·68 ± 0·65 mm, the eyes were highly pigmented and the teeth were visible. These are the first reported findings on the initial developmental stages of B. gouldingi and could be used to improve captive breeding management and conservation practices.

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“…During fertilization, a large number of spermatozoa compete to fertilize a single oocyte, as observed at the micropylar region in the hybrid egg 30 seconds after fertilization. Freire-Brasil (2001) and Ganeco et al (2008), analyzing fertilization in Prochilodus lineatus and Brycon orbinyanus, reported that several spermatozoa reach and pass through the micropyle, reinforcing the development of distinct strategies in fish to avoid polyspermy. Hart (1990) pointed out some of them, such as: the presence of chorion as well as micropyle, modifications in the site of spermatozoon entrance, obstruction of the micropylar canal and inhibition of spermatozoa by fluids from the perivitelline space.…”

Section: B C D E Fmentioning

confidence: 99%

Structural and ultrastructural characterization of the embryonic development of Pseudoplatystoma spp. hybrids

Faustino

Nakaghi²,

Marques³

et al. 2010

Int. J. Dev. Biol.

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The hybrid fish Pseudoplatystoma spp. has been raised on a large scale by several fish farmers, despite the fact that little is known about its biology. This is because it presents a number of zootechnical advantages over the parental species. In order to provide information about the early morphology of this important species, we analyzed the fertilization and embryonic development of the hybrid between spotted females and barred males of sorubim specimens by light microscopy and by scanning (SEM) and transmission electron microscopy (TEM) after induced spawning. Samples were collected at pre-established moments up to larval hatching. Seven distinct stages of hybrid embryonic development were identified: zygote, cleavage, morula, blastula, gastrula, histogenesis and organogenesis, and hatching. Under SEM, we observed spermatozoa at the micropyle entrance, the formation of a fertilization cone in the eggs, the differentiation of cephalic and caudal regions, the neural tube and embryo growth along the cephalo-caudal axis, as well as rudimentary optic vesicle and barbels. Under light microscopy, cytoplasmic movement was apparent with the consequent formation of animal and vegetative poles in eggs, in addition to epiboly movements and a small notochord portion. Under TEM, the oocyte chorion and eggs presented a sieve-like aspect in transversal cuts, coupled with the rupture of cortical alveoli and chorion elevation, thus enlarging the perivitelline space. Several mitochondria in the cortical cytoplasm were detected in both oocytes and eggs. Overall, we observed that the larvae hatched without visible morphological alterations, and seemed to be as viable in captive systems as they are in the natural environment.

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Structural analysis of fertilization in the fish Brycon orbignyanus

Cited by 17 publications

References 29 publications

Comparative Analysis of the Oocytes and Early Development of Two Species of Curimatidae Teleost Fish

Comparative Analysis of the Oocytes and Early Development of Two Species of Curimatidae Teleost Fish

Morphological and morphometric aspects of early life stages of piabanhaBrycon gouldingi(Characidae)

Structural and ultrastructural characterization of the embryonic development of Pseudoplatystoma spp. hybrids

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