Structural Analysis of Antioxidative Peptides from Soybean .beta.-Conglycinin

Chen, Hua Ming; Muramoto, Koji; Yamauchi, Fumio

doi:10.1021/jf00051a004

Cited by 533 publications

(396 citation statements)

References 15 publications

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“…Karnjanapratum and Benjakul (2014a) also reported that H 2 O 2 scavenging activity of gelatin hydrolysates from unicorn leatherjacket prepared using papain decreased when the level of papain increased. It was reported that degree of hydrolysis had no relationship with antioxidative activity of protein hydrolysate (Chen et al 1995). The extensive hydrolysis probably caused the lower antioxidative activity, due to the generation of free amino acids which had lower antioxidative activity, compared to peptides (Hernandez- Ledesma et al 2005).…”

Section: Resultsmentioning

confidence: 99%

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Karnjanapratum

Benjakul

2015

Int Aquat Res

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Gelatin hydrolysates from autolysed non-swollen and swollen unicorn leatherjacket skin prepared using partially purified glycyl endopeptidase (GE) from papaya latex were examined for their antioxidative activities. Autolysed swollen skin was more hydrolysed by GE as indicated by higher a-amino group content, compared with autolysed non-swollen skin. ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of hydrolysates were increased with increasing levels of GE used. Antioxidative gelatin hydrolysate from autolysed skin, both non-swollen and swollen skins, using 8 % GE termed 'NS-8GE' and 'SS-8GE' exhibited different modes of action. When both hydrolysates were tested in lecithin liposome system, the efficiency in retardation of lipid oxidation was in a dose-dependent manner. Antioxidative activity of hydrolysates at 5.0 g/L was comparable to that of 0.1 g/L Trolox, in which the oxidation was almost completely inhibited. NS-8GE and SS-8GE also showed their antioxidative activities in gastrointestinal tract model system (GIMs). ABTS radical scavenging activity of both hydrolysates increased, but FRAP decreased in a duodenal condition. Based on size exclusion chromatography, major antioxidative peptides in NS-8GE and SS-8GE had molecular weight of 1,170 and 750 Da, respectively. Therefore, GE could enhance antioxidative activity of autolysed skin. Additionally, swelling process directly determined the modes of actions of resulting gelatin hydrolysates. NS-8GE had high ABTS radical scavenging activity, whereas SS-8GE showed high FRAP.

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Section: Resultsmentioning

confidence: 99%

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Karnjanapratum

Benjakul

2015

Int Aquat Res

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“…A number of commercial proteases have been used for the production of these hydrolysates and peptides (Lin and Li 2006;Mendis et al 2005;Yang et al 2008). Protease specificity affects size, amount, free amino acid composition and, peptides and their amino acid sequences, which in turn influences the biological activity of the hydrolysates (Chen et al 1995;Jeon et al 1999;Wu et al 2003). Considering the fact that microbial-derived proteinases are low-cost and safe, and the product yields are very high, the use of these enzymes to hydrolyze food proteins is valuable (Phelan et al 2009).…”

Section: Degree Of Hydrolysis (%)mentioning

confidence: 99%

ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

et al. 2012

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Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cutoff (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F 3 permeates (MWCO< 1 kDa) of skin (IC 50 03.2 μg/ml) and bone (IC 50 01.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F 3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.

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“…Peptide antioxidative properties are related to their amino acid composition, structure and molecular weight that low molecular weight peptides possess a strong antioxidant activity [20,21]. Protease specificity can affect size, amount, free amino acid composition of peptides and their amino acid sequences, which in turn influences the biological activity of the hydrolysates [20,22,23].…”

Section: Preparation Of Sea Cucumber Peptidesmentioning

confidence: 99%

Antioxidant peptides isolated from sea cucumber Stichopus Japonicus

Zhou

Wang

Ai-li

2011

Eur Food Res Technol

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The sea cucumber Stichopus japonicus protein was hydrolyzed by pepsin, trypsin, papain, acid protease and neutral protease, respectively, to get five kinds of peptide fractions: pepsin peptides (PP), trypsin peptides (TP), acid protease peptides (AP), neutral protease peptides (NP) and papain peptide (PAP). Antioxidative activities of all peptide fractions were evaluated by hydroxyl radical-(ÁOH) and Superoxide anion (O 2 Á 2 )-scavenging activity.Trypsin peptide (TP) exhibited the highest antioxidative activity compared to other peptide fractions. In considering scavenging effects on hydroxyl radicals (ÁOH) and Superoxide anions (O 2 Á 2 ), TP was employed for isolation, purification and identification of antioxidant peptide. To purify and characterize antioxidative peptide, two steps gel filtration, one-step ion-exchange column chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) were used. The purified antioxidative peptide TP2b-1 was a novel peptide and was sequenced as GPEPTGPTGAPQWLR, in which the low molecular weight and some amino acid constituents played important role in the radical-scavenging effects according reports. The IC 50 values of TP2b-1 were 138.9 lM on ÁOH and 353.9 lM on O 2 Á 2 .

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Structural Analysis of Antioxidative Peptides from Soybean .beta.-Conglycinin

Cited by 533 publications

References 15 publications

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

Antioxidative gelatin hydrolysate from unicorn leatherjacket skin as affected by prior autolysis

ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

Antioxidant peptides isolated from sea cucumber Stichopus Japonicus

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