Structural analysis of an extracellular polysaccharide produced by Rhodococcus rhodochrous strain S-2

Urai, Makoto; Anzai, H.; Ogihara, Jun; Iwabuchi, Noriyuki; Harayama, Shigeaki; Sunairi, Michio; Nakajima, Mizuki

doi:10.1016/j.carres.2005.12.013

Cited by 45 publications

(27 citation statements)

References 24 publications

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“…The S-2 EPS was prepared as described by Urai et al [5]. Hyaluronic acid from cockscomb was purchased from Tokyo Kasei Kogyo Co. (Tokyo, Japan), and the molecular sieve, glycerol, silica gel, and urea were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The native S-2 EPS contains 0.8% (W/W) stearic acid and 2.7% (W/W) palmitic acid as described previously [5]. To elucidate the functions of the fatty acid residues of S-2 EPS, the residues were removed by mild alkali hydrolysis (deacylated S-2 EPS, DeAcyl S-2 EPS), and the deacylated S-2 EPS was reacylated by palmitoylation (ReAcyl S-2 EPS).…”

Section: Chemical Modification Of S-2 Epsmentioning

confidence: 99%

“…Recently, we determined the sugar-chain structure of one of these rhodococcal FACEPS, the EPS produced by R. rhodochrous strain S-2 (S-2 EPS) [5]. S-2 EPS consists of a tetrasaccharide repeating unit with an → 3)-α-D-mannose-(1 → 3)-α-D-glucose-(1 → 3)-α-D-galactose-(1 → backbone with side chains of β-D-glucuronic acid residues bound to the C-2 position of the mannose residue via glycosyl bond, and it contains 0.8% (W/W) stearic acid and 2.7% (W/W) palmitic acid.…”

Section: Introductionmentioning

confidence: 99%

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An Anionic Polymer Incorporating Low Amounts of Hydrophobic Residues Is a Multifunctional Surfactant. Part 1: Emulsifying, Thickening,Moisture-Absorption and Moisture-Retention Abilities of a FattyAcid-Containing Anionic Polysaccharide

Urai¹,

Aizawa²,

Nakajima³

et al. 2015

ACES

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We have been studying the function and structure of fatty acid-containing extracellular polysaccharides (FACEPS) produced by bacteria belonging to the genus Rhodococcus. In this study, we examined the relationships between the structure and emulsifying, thickening, moisture-absorption, and moisture-retention capabilities of rhodococcal FACEPS using S-2 EPS produced by R. rhodochrous strain S-2. We prepared chemically deacylated S-2 EPS (DeAcyl S-2 EPS) and palmitoylated DeAcyl S-2 EPS (ReAcyl S-2 EPS), and compared them with native S-2 EPS. All of the properties were attenuated and recovered by deacylation and reacylation of S-2 EPS, respectively. These results suggest that the fatty acid moiety of rhodococcal FACEPS is involved in such functional properties. We also showed that palmitoylation improved the emulsifying, moisture-absorption, and moisture-retention abilities of other acidic polysaccharides that are commercially available. These results suggest that the acidity of the polysaccharide backbone is at least partly responsible for the observed functionality of fatty acid-containing polysaccharides. To our knowledge, this is the first report on multifunctional property of an anionic polymer incorporating low * Corresponding author. M. Urai et al. 174amounts of hydrophobic residues. The present findings could be useful for the creation of new multifunctional surfactants from renewable raw materials for use in various industries, e.g., in cosmetics.

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Section: Methodsmentioning

confidence: 99%

Section: Chemical Modification Of S-2 Epsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Anionic Polymer Incorporating Low Amounts of Hydrophobic Residues Is a Multifunctional Surfactant. Part 1: Emulsifying, Thickening,Moisture-Absorption and Moisture-Retention Abilities of a FattyAcid-Containing Anionic Polysaccharide

Urai¹,

Aizawa²,

Nakajima³

et al. 2015

ACES

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“…To confirm the homogeneity of M2 EPS, gelfiltration column chromatography was performed on a Sephacryl S1000 (850 mm × 15 mm; Amersham Biosciences UK Ltd., Buckinghamshire, UK) column with 1 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl as the eluent (17). Dextran T2000 (Amersham Biosciences UK Ltd., Buckinghamshire, UK) was used as a size marker.…”

Section: Purification Of Innate Immune Stimulant From Rhizobium Sp Smentioning

confidence: 99%

“…M2 EPS was extracted from the supernatant of the suspension as described previously (17), except the Sevag method was used instead of phenol-chloroform treatment (18). M2 EPS was further purified by DEAE-Toyopearl 650 M column chromatography as described previously (19).…”

Section: Purification Of M2 Epsmentioning

confidence: 99%