“…However, since the constant temperature trajectories are discontinuous, the RE sampling method loses kinetic information. Furthermore, since the number of replicas increases markedly with system’s size, the use of RE-MD is limited to the study of oligomerization of small peptide fragments ( Nishino et al, 2005 ; Liang et al, 2007 , 2008 ; Wei et al, 2007 ; De Simone and Derreumaux, 2010 ; Nishikawa et al, 2019 ). In the case of β2m, MD simulations have been used to study the dynamics of the native monomer ( Ma and Nussinov, 2003 ; Deng et al, 2006 ; Fogolari et al, 2011 ; Chandrasekaran and Rajasekaran, 2016 ), the oligomerization process of selected amyloidogenic β2m peptide sequences ( Liang et al, 2007 ; Zheng et al, 2008 ; Fang et al, 2009a ), and provide complementary information to that obtained through in vitro experiments ( Gumral et al, 2013 ; Chong et al, 2015 ; Torbeev et al, 2015 ; Camilloni et al, 2016 ; Hall et al, 2016 ; Brancolini et al, 2018 ).…”