Structural analysis and binding domain of albumin complexes with natural dietary supplement humic acid

Ding, Fei; Diao, Jian-Xiong; Yang, Xinling; Sun, Ying

doi:10.1016/j.jlumin.2011.06.005

Cited by 15 publications

(4 citation statements)

References 45 publications

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“…Overall, the fluorescence spectroscopy indicates that the binding of the gold complexes to the HSA proceeds through a single interaction mode that strongly affects the protein emission, consistent with the formation of a non-fluorescent 1:1 adduct in the Overall, the fluorescence spectroscopy indicates that the binding of the gold complexes to the HSA proceeds through a single interaction mode that strongly affects the protein emission, consistent with the formation of a non-fluorescent 1:1 adduct in the ground state for all complexes, except for the complex [P][AuSEt(=Se)] which seems to form a non-fluorescent 1:2 adduct. The binding constants determined for these four complexes are higher than that found for Cisplatin (10 2 M −1 ) and similar to those found for other gold complexes (10 3 −10 4 M −1 ) [67][68][69][70][71].…”

Section: Gold-complexes-hsa Interaction Studiessupporting

confidence: 82%

Broad Spectrum Functional Activity of Structurally Related Monoanionic Au(III) Bis(Dithiolene) Complexes

Gal

Filatre-Furcate

Lorcy

et al. 2022

IJMS

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The biological properties of sixteen structurally related monoanionic gold (III) bis(dithiolene/diselenolene) complexes were evaluated. The complexes differ in the nature of the heteroatom connected to the gold atom (AuS for dithiolene, AuSe for diselenolene), the substituent on the nitrogen atom of the thiazoline ring (Me, Et, Pr, iPr and Bu), the nature of the exocyclic atom or group of atoms (O, S, Se, C(CN)2) and the counter-ion (Ph4P+ or Et4N+). The anticancer and antimicrobial activities of all the complexes were investigated, while the anti-HIV activity was evaluated only for selected complexes. Most complexes showed relevant anticancer activities against Cisplatin-sensitive and Cisplatin-resistant ovarian cancer cells A2780 and OVCAR8, respectively. After 48 h of incubation, the IC50 values ranged from 0.1–8 µM (A2780) and 0.8–29 µM (OVCAR8). The complexes with the Ph4P+ ([P]) counter-ion are in general more active than their Et4N+ ([N]) analogues, presenting IC50 values in the same order of magnitude or even lower than Auranofin. Studies in the zebrafish embryo model further showed that, despite their marked anticancer effect, the complexes with [P] counter-ion exhibited low in vivo toxicity. In general, the exocyclic exchange of sulfur by oxygen or ylidenemalononitrile (C(CN)2) enhanced the compounds toxicity. Most complexes containing the [P] counter ion exhibited exceptional antiplasmodial activity against the Plasmodium berghei parasite liver stages, with submicromolar IC50 values ranging from 400–700 nM. In contrast, antibacterial/fungi activities were highest for most complexes with the [N] counter-ion. Auranofin and two selected complexes [P][AuSBu(=S)] and [P][AuSEt(=S)] did not present anti-HIV activity in TZM-bl cells. Mechanistic studies for selected complexes support the idea that thioredoxin reductase, but not DNA, is a possible target for some of these complexes. The complexes [P] [AuSBu(=S)], [P] [AuSEt(=S)], [P] [AuSEt(=Se)] and [P] [AuSeiPr(=S)] displayed a strong quenching of the fluorescence intensity of human serum albumin (HSA), which indicates a strong interaction with this protein. Overall, the results highlight the promising biological activities of these complexes, warranting their further evaluation as future drug candidates with clinical applicability.

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Section: Gold-complexes-hsa Interaction Studiessupporting

confidence: 82%

Broad Spectrum Functional Activity of Structurally Related Monoanionic Au(III) Bis(Dithiolene) Complexes

Gal

Filatre-Furcate

Lorcy

et al. 2022

IJMS

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“…Encapsulation of trypsin and ribonuclease A, which to the contrary bear negatively charged surfaces, suggested that hydrophobic effects can overcome localized charge repulsion when the pH is sufficiently low to reduce surface charge density, fostering entrapment within the HA matrix (Tomaszewski et al, 2011). Encapsulation enhances resistance against proteases (Zang et al, 2000) and preserves activity of soil enzymes, albeit at lower levels, by inducing conformational changes (Ding et al, 2011) and/or hampering access of substrates to the active site (Chen et al, 2004b).…”

Section: Reduction In Infectivity Of Soil Prionsmentioning

confidence: 99%

Using Humic Fractions to Understand Natural Organic Matter Processes in Soil and Water: Selected Studies and Applications

et al. 2019

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Natural organic matter (NOM) plays key environmental roles in both aquatic and soil systems. A long-standing approach for evaluating NOM composition and activity is to extract soils with alkali solutions to obtain humic substances, namely humic acids (HA), and fulvic acids (FA), or to briefly expose isolated fractions of dissolved organic matter to alkali. Critics have claimed these methods create laboratory artifacts and are thus unsuitable for studying NOM behavior in field conditions. In response, we describe case studies in which humic fractions were analyzed to identify significant processes in environmental or agricultural issues. Specifically, humic fractions played a key role in maintaining toxic levels of arsenic (As) in drinking water supplies in South and Southeast Asia. Elsewhere, binding reactions of FA and HA with prions were shown to provide a plausible mechanism for variable persistence of prion infectivity across soil types. Humic substances were also shown to enhance iron (Fe) uptake by plants in solution culture and field conditions. Their specific binding sites for mercury (Hg) as determined in laboratory conditions enabled accurate modeling of soil Hg binding under varying conditions. A young HA fraction reproduced in controlled conditions the capacity of animal manure to maintain potassium (K) availability in strongly K-fixing field soils, leading to development of a commercially successful humic-K fertilizer. Humic fractions accurately represented NOM across multiple settings and research objectives while providing novel opportunities for advanced analyses. The study of humic fractions has helped resolve scientific and practical issues in aquatic and soil systems.

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“…Interaction studies of HSA with biological molecules can elucidate the properties of the drug-protein complex because they may provide useful information about the structural features that determine the therapeutic effectiveness of drugs. Therefore, various studies on the interactions between HSA and small bioactive molecules have been reported over the years [16][17][18][19][20]. Generally, strong binding to HSA will decrease the concentrations of a free drug in plasma, whereas weak binding will reduce the duration of drug action.…”

Section: Introductionmentioning

confidence: 99%

Comparative Interactions of Dihydroquinazolin Derivatives with Human Serum Albumin Observed via Multiple Spectroscopy

et al. 2017

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Abstract:The interactions of dihydroquinazolines with human serum albumin (HSA) were studied in pH 7.4 aqueous solution via fluorescence, circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopic techniques. In this work, 6-chloro-1-(3,3-dimethyl-butanoyl)-2-(un)substitutedphenyl-2,3-dihydroquinazolin-4(1H)-one (PDQL) derivatives were designed and synthesized to study the impact of five similar substituents (methyl, methoxy, cyano, trifluoromethyl and isopropyl) on the interactions between PDQL and HSA using a comparative methodology. The results revealed that PDQL quenched the intrinsic fluorescence of HSA through a static quenching process. Displacement experiments with site-specific markers revealed that PDQL binds to HSA at site II (subdomain IIIA) and that there may be only one binding site for PDQL on HSA. The thermodynamic parameters indicated that hydrophobic interactions mainly drove the interactions between PDQL and HSA. The substitution using five similar groups in the benzene ring could increase the interactions between PDQL and HSA to some extent through the van der Waals force or hydrogen bond effects in the proper temperature range. Isopropyl substitution could particularly enhance the binding affinity, as observed via comparative studies.

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Structural analysis and binding domain of albumin complexes with natural dietary supplement humic acid

Cited by 15 publications

References 45 publications

Broad Spectrum Functional Activity of Structurally Related Monoanionic Au(III) Bis(Dithiolene) Complexes

Broad Spectrum Functional Activity of Structurally Related Monoanionic Au(III) Bis(Dithiolene) Complexes

Using Humic Fractions to Understand Natural Organic Matter Processes in Soil and Water: Selected Studies and Applications

Comparative Interactions of Dihydroquinazolin Derivatives with Human Serum Albumin Observed via Multiple Spectroscopy

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