Strontium ranelate-loaded PLGA porous microspheres enhancing the osteogenesis of MC3T3-E1 cells

Mao, Zhenyang; Fang, Zhiwei; Yang, Yunqi; Chen, Xuan; Wang, Yugang; Kang, Jian; Qu, Xinhua; Yuan, Weien; Dai, Kerong

doi:10.1039/c7ra01445g

Cited by 23 publications

(15 citation statements)

References 92 publications

(99 reference statements)

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“…[8][9][10][11] MSCs have been the focus of recent studies in bone tissue engineering, in which they are seeded in a scaffold and induced to generate new osteogenic cells via osteoinductive cues. [12][13][14] Appropriate bioactive cues encapsulated within the scaffolds can not only regulate cell adhesion and proliferation, but also enhance osteogenic differentiation. For example, incorporation of hydroxyapatite (HA) into the formulations of scaffolds enhanced the osteoinductivity 15,16 since bone is primarily composed of HA 17 .…”

Section: Introductionmentioning

confidence: 99%

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

et al. 2018

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Section: Introductionmentioning

confidence: 99%

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

et al. 2018

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“…There are some studies of porous scaffolds via mechanically mixing β-TCP/β-CaSi with different β-TCP mass fraction 17 , 19 – 21 but little of research on excellent osteogenic capability has been confirmed. On the other hand, granular form of bioceramics has an advantage of suiting for non-geometrically well-defined craniomaxillofacial bone defects accompanied with favorable interconnected macropore structures and is also potentially used as drug delivery systems 22 , 23 . Recently, we have developed a gradient hybrid design to fabricate core-shell β-TCP‒β-CaSi (CaP@CaSi, CaSi@CaP) microspheres conceiving respective biological performance to be exerted in specific layer 24 .…”

Section: Introductionmentioning

confidence: 99%

Optimized Bone Regeneration in Calvarial Bone Defect Based on Biodegradation-Tailoring Dual-shell Biphasic Bioactive Ceramic Microspheres

Chen

et al. 2018

Sci Rep

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Bioceramic particulates capable of filling bone defects have gained considerable interest over the last decade. Herein, dual-shell bioceramic microspheres (CaP@CaSi@CaP, CaSi@CaP@CaSi) with adjustable beta-tricalcium phosphate (CaP) and beta-calcium silicate (CaSi) distribution were fabricated using a co-concentric capillary system enabling bone repair via a tailorable biodegradation process. The in vitro results showed the optimal concentration (1/16 of 200 mg/ml) of extracts of dual-shell microspheres could promote bone marrow mesenchymal cell (BMSC) proliferation and enhance the level of ALP activity and Alizarin Red staining. The in vivo bone repair and microsphere biodegradation in calvarial bone defects were compared using micro-computed tomography and histological evaluations. The results indicated the pure CaP microspheres were minimally resorbed at 18 weeks post-operatively and new bone tissue was limited; however, the dual-shell microspheres were appreciably biodegraded with time in accordance with the priority from CaSi to CaP in specific layers. The CaSi@CaP@CaSi group showed a significantly higher ability to promote bone regeneration than the CaP@CaSi@CaP group. This study indicates that the biphasic microspheres with adjustable composition distribution are promising for tailoring material degradation and bone regeneration rate, and such versatile design strategy is thought to fabricate various advanced biomaterials with tailorable biological performances for bone reconstruction.

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“…SrR, a classic anti-osteoporosis drug, has been gradually recognized to have an anti-osteoarthritis effect and the scope of its clinical application has gradually expanded in recent years. The role of SrR in promoting osteogenesis (18,(24)(25)(26), chondrogenesis (14,15,18,27) and angiogenesis (21,28) has been experimentally confirmed, but its anti-inflammatory Figure 1. Effect of SrR on rat chondrocytes viability.…”

Section: Discussionmentioning

confidence: 99%

The anti‑inflammation effect of strontium ranelate on rat chondrocytes with or without IL‑1β in vitro

Liu

Yang

et al. 2022

Exp Ther Med

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Temporomandibular joint osteoarthritis (TMJ-OA) is a common disease with a high level of inflammation in the joint micro-environment and cartilage degradation. Anti-inflammation and cartilage regeneration are the key therapies for TMJ-OA, but currently, there are no novel medicines or treatments that can control its pathogenic progression. Strontium ranelate (SrR) is an anti-osteoporosis drug and is now considered a promising anti-OA drug, but the anti-inflammatory effect of SrR remains to be elucidated. In the present study, the anti-inflammatory effect of SrR in a normal or high IL-1β environment was observed. Cell viability under the treatment of SrR was tested using Cell Counting Kit-8. Toluidine blue staining, immunofluorescence staining, hydroxyproline assay, PCR assay and western blotting were used to detect the expression of collagen (Col)II, proteoglycans (PG) and aggrecan as a reflection of extracellular matrix synthesis and MMP-9,13 hydroxyproline was used as an inflammation indicator. IL-1β of 10 ng/ml was added to the culture medium as inflammation environment and the tests of those biomarkers were done again. Then, the changes in β-catenin were also studied by immunofluorescence staining, PCR assay and western blotting to explore the possible involvement of the Wnt/β-catenin pathway. The results showed a significant inhibition of MMP-9, MMP-13, β-catenin and promotion of Col-II, PG and aggrecan in normal chondrocytes. The presence of IL-1β markedly upregulated the expression of MMP-9, MMP-13 and β-catenin while suppressing Col-II and PG and SrR partially reversed this trend. In conclusion, SrR decreased MMPs but promoted Col-II, aggrecan and PG synthesis in rat chondrocytes with or without the presence of IL-1β and SrR attenuated the IL-1β-induced increase in β-catenin, thus reducing the inflammatory reaction.

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Strontium ranelate-loaded PLGA porous microspheres enhancing the osteogenesis of MC3T3-E1 cells

Abstract: Biodegradable poly lactic-co-glycolic acid (PLGA) has been used as a tissue engineering scaffold as well as a carrier for the delivery of proteins, drugs, and other macromolecules.

Cited by 23 publications

References 92 publications

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Optimized Bone Regeneration in Calvarial Bone Defect Based on Biodegradation-Tailoring Dual-shell Biphasic Bioactive Ceramic Microspheres

The anti‑inflammation effect of strontium ranelate on rat chondrocytes with or without IL‑1β in vitro

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