Strontium hydroxyapatite/chitosan nanohybrid scaffolds with enhanced osteoinductivity for bone tissue engineering

Lei, Yong; Xu, Zhi-Fang; Ke, Qinfei; Yin, Wenwu; Chen, Yixuan; Zhang, Changqing; Guo, Ya‐Jun

doi:10.1016/j.msec.2016.11.063

Cited by 150 publications

(90 citation statements)

References 47 publications

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“…Our results were similar to that Wu [47], who demonstrated that Si up-regulated the osteogenic-related gene expression of COL, ALP, and RUNX2 in the stem cells. Moreover, Si and Sr ions can activate Ca sensing receptor and improve osteogenic-related proteins production [48]. Also, Shaltooki et al reasoned that the scaffolds reinforced with Sr-bioglass exhibited a significant increase in the ALP activity in comparison with the scaffolds containing pure bioglass [49].…”

Section: Osteogenesismentioning

confidence: 99%

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Wang

Liu

et al. 2020

Applied Sciences

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Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications. Furthermore, bone regeneration is a complex physiological process regulated by osteoclast resorption with osteoblast bone formation and the entire process includes inflammatory reactions, endochondral bone formation and bone remodeling. Currently, for clinical cases, the golden method to repair bone defects is to use autologous bone grafts as this method avoids disease spreading and immunological rejection. However, autografts have some major drawbacks such as limited availability and invasive harvesting that requires additional surgery to harvest healthy bones from another site. Furthermore, additional surgeries mean increased surgical risks and complications [2]. Allograft is an alternative to bone repair; however, it is highly limited due to rejection and lack of availability. To solve this problem, researchers had attempted to develop novel biomaterials including ceramics, polymers and composites as an alternative choice for bone regeneration [3][4][5]. In order to promote bone healing, the material must be biocompatible, biodegradable and able to promote bone regeneration which mimics the distinctive property of natural bone [6]. With the emergence of newer 3D printing techniques, we are now able to fabricate scaffolds with customized shape, pore sizes and architectures that are similar to native bone [7,8].For many years, bioceramics has emerged as promising biomaterial for bone regeneration [9,10]. Calcium phosphate is one of the most popular bioceramics which had been repeate...

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Section: Osteogenesismentioning

confidence: 99%

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Wang

Liu

et al. 2020

Applied Sciences

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“…For example, nanostructured LDHs can serve as drug delivery system, which exhibit the long‐term stability and storage of the drug by isolating it from a hostile environment . Chitosan (CS) is a typical natural material with great advantages in bone tissue engineering, which provides a friendly microenvironment for cell proliferation and extracellular matrix production . Up to now, various organic–inorganic composite scaffolds have been developed because they possess more advantages in mechanical and biological properties than pure organic or inorganic biomaterials .…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11] Chitosan (CS) is a typical natural material with great advantages in bone tissue engineering, which provides a friendly microenvironment for cell proliferation and extracellular matrix production. [12][13][14] Up to now, various organic-inorganic composite scaffolds have been developed because they possess more advantages in mechanical and biological properties than pure organic or inorganic biomaterials. 15,16 To the best of our knowledge, LDH/CS composite scaffolds have rarely reported for the application of bone tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Cao

Chen

et al. 2017

J Biomed Mater Res

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Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection. Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold. This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm. The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces. Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs. When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property. The released Sr ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2. Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus. Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.

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“…Despite its excellent biological properties, CH exhibits low mechanical properties, limiting its suitability for orthopedic implants [17]. Therefore, a proposed novel approach for a CH/ceramics scaffold is a better candidate for bone tissue engineering due to its excellent physicochemical and biological properties [18,19]. …”

Section: Introductionmentioning

confidence: 99%

Calcium Silicate/Chitosan-Coated Electrospun Poly (Lactic Acid) Fibers for Bone Tissue Engineering

Su¹,

Wei

et al. 2017

Materials

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Electrospinning technology allows fabrication of nano- or microfibrous fibers with inorganic and organic matrix and it is widely applied in bone tissue engineering as it allows precise control over the shapes and structures of the fibers. Natural bone has an ordered composition of organic fibers with dispersion of inorganic apatite among them. In this study, poly (lactic acid) (PLA) mats were fabricated with electrospinning and coated with chitosan (CH)/calcium silicate (CS) mixer. The microstructure, chemical component, and contact angle of CS/CH-PLA composites were analyzed by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, various CS/CH-coated PLA mats increased the formation of hydroxyapatite on the specimens’ surface when soaked in cell cultured medium. During culture, several biological characteristics of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-PLA groups were promoted as compared to those on pure PLA mat. Increased secretion levels of Collagen I and fibronectin were observed in calcium silicate-powder content. Furthermore, with comparison to PLA mats without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs and their osteogenesis properties, which was characterized by osteogenic-related gene expression. These results clearly demonstrated that the biodegradable and electroactive CS/CH-PLA composite mats are an ideal and suitable candidate for bone tissue engineering.

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Strontium hydroxyapatite/chitosan nanohybrid scaffolds with enhanced osteoinductivity for bone tissue engineering

Cited by 150 publications

References 47 publications

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Calcium Silicate/Chitosan-Coated Electrospun Poly (Lactic Acid) Fibers for Bone Tissue Engineering

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