Strontium-Doped Bioactive Glass Nanoparticles in Osteogenic Commitment

Leite, Álvaro J.; Gonçalves, Ana I.; Rodrigues, Márcia T.; Gomes, Manuela E.; Mano, João F.

doi:10.1021/acsami.8b06154

Cited by 58 publications

(36 citation statements)

References 59 publications

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“…Such effect has not been observed for other divalent cations. On the basis of this dual effect, several Sr 2+ -based medicines [12][13][14] and biomaterials [15,16] have been developed to treat osteoporosis, a bone disease that affects hundreds of millions of people worldwide [17,18]. However, Sr 2+ administration has a dose-dependent impact on bone formation [19] and high Sr 2+ doses have been associated with the development of skeletal diseases like osteomalacia in rats with renal failure [20,21].…”

Section: Introductionmentioning

confidence: 99%

Formation of stable strontium-rich amorphous calcium phosphate: Possible effects on bone mineral

et al. 2019

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a b s t r a c tBone, tooth enamel, and dentin accumulate Sr 2+ , a natural trace element in the human body. Sr 2+ comes from dietary and environmental sources and is thought to play a key role in osteoporosis treatments. However, the underlying impacts of Sr 2+ on bone mineralization remain unclear and the use of synthetic apatites (which are structurally different from bone mineral) and non-physiological conditions have led to contradictory results. Here, we report on the formation of a new Sr 2+ -rich and stable amorphous calcium phosphate phase, Sr(ACP). Relying on a bioinspired pathway, a series of Sr 2+ substituted hydroxyapatite (HA) that combines the major bone mineral features is depicted as model to investigate how this phase forms and Sr 2+ affects bone. In addition, by means of a comprehensive investigation the biomineralization pathway of Sr 2+ bearing HA is described showing that not more than 10 at% of Sr 2+ , i.e. a physiological limit incorporated in bone, can be incorporated into HA without phase segregation. A combination of 31 P and 1 H solid state NMR, energy electron loss spectromicroscopy, transmission electron microscopy, electron diffraction, and Raman spectroscopy shows that Sr 2+ introduces disorder in the HA culminating with the unexpected Sr(ACP), which co-exists with the HA under physiological conditions. These results suggest that heterogeneous Sr 2+ distribution in bone is associated with regions of low structural organization. Going further, such observations give clues from the physicochemical standpoint to understand the defects in bone formation induced by high Sr 2+ doses. Statement of SignificanceUnderstanding the role played by Sr 2+ has a relevant impact in physiological biomineralization and provides insights for its use as osteoporosis treatments. Previous studies inspired by the bone remodelling pathway led to the formation of biomimetic HA in terms of composition, structures and properties in water. Herein, by investigating different atomic percentage of Sr 2+ related to Ca 2+ in the synthesis, we demonstrate that 10% of Sr 2+ is the critical loads into the biomimetic HA phase; similarly to bone. Unexpectedly, using higher amount leads to the formation of a stable Sr 2+ -rich amorphous calcium phosphate phase that may high-dose related pathologies. Our results provide further understanding of the different ways Sr 2+ impacts bone.⇑ Corresponding authors at

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Section: Introductionmentioning

confidence: 99%

Formation of stable strontium-rich amorphous calcium phosphate: Possible effects on bone mineral

et al. 2019

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“…The used ions were expected to have a positive influence on osteogenic differentiation, because of their important role in bone biology [19][20][21]. An increased osteogenic differentiation in the presence of ions like Ca, Sr and Mg has been shown in previous studies [22,23]. An increased extracellular calcium concentration can cause an increase of intracellular calcium through calcium channels and results in activation of important targets during osteogenic differentiation [24].…”

Section: Cell Response In the Presence Of Released Ionsmentioning

confidence: 94%

Grain boundary corrosion in TiO2 bone scaffolds doped with group II cations

Klemm

Gomez‐Florit²,

Carvalho

et al. 2019

Journal of the European Ceramic Society

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A pH drop during the inflammatory phase during bone regeneration can cause corrosion in TiO 2 bone scaffolds and the loss of compressive strength. Corrosion as ion leaching and dissolution is confined to grain boundaries. Cationic doping of TiO 2 showed to increase the compressive strength but increased the amount of impurities in grain boundaries as well. Therefore, this study showed the different grain boundary formation for Ca, Sr and Mg doped scaffolds and their corrosion behavior. After corrosion, the amorphous phase in grain boundaries was dissolved in all doped scaffolds. Differences occurred due to the formation of an additional crystalline phase in Sr doped scaffolds. The presence of an amorphous and crystalline phase led to an inhomogeneous dissolution in grain boundaries and a significant decrease in compressive strength already after 4 h in contact with an acidic environment. Released ions did not show any cytotoxic effect on hASCs. Mg doped TiO 2 scaffolds led to significant increased osteogenic differentiation.

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“…The results of Leite et al showed that the products generated by the dissolved nanoparticles promoted the expression of key genes and proteins associated with osteogenic lineages in hASCs. This effect has been significantly improved by the presence of strontium, which induces osteogenic differentiation even without the use of osteogenic inducers [ 308 ].…”

Section: Adipose Stem Cells and Regenerative Medicinementioning

confidence: 99%

Adipose Stem Cell Translational Applications: From Bench-to-Bedside

Argentati

Morena

Bazzucchi

et al. 2018

IJMS

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During the last five years, there has been a significantly increasing interest in adult adipose stem cells (ASCs) as a suitable tool for translational medicine applications. The abundant and renewable source of ASCs and the relatively simple procedure for cell isolation are only some of the reasons for this success. Here, we document the advances in the biology and in the innovative biotechnological applications of ASCs. We discuss how the multipotential property boosts ASCs toward mesenchymal and non-mesenchymal differentiation cell lineages and how their character is maintained even if they are combined with gene delivery systems and/or biomaterials, both in vitro and in vivo.

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Strontium-Doped Bioactive Glass Nanoparticles in Osteogenic Commitment

Cited by 58 publications

References 59 publications

Formation of stable strontium-rich amorphous calcium phosphate: Possible effects on bone mineral

Formation of stable strontium-rich amorphous calcium phosphate: Possible effects on bone mineral

Grain boundary corrosion in TiO2 bone scaffolds doped with group II cations

Adipose Stem Cell Translational Applications: From Bench-to-Bedside

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