Strontium Ameliorates Glucocorticoid Inhibition of Osteogenesis Via the ERK Signaling Pathway

Aimaiti, Abudousaimi; Wahafu, Tuerhongjiang; Keremu, Ajimu; Li, Yicheng; Cao, Li

doi:10.1007/s12011-019-02009-6

Cited by 14 publications

(10 citation statements)

References 45 publications

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“…Peng et al showed that strontium could greatly increase RUNX2 transcriptional activity and phosphorylation [ 22 ]. Aimaiti pointed out that strontium could enhance osteogenesis and matrix mineralization via the ERK signaling pathway [ 23 ]. ERK signaling pathways can activate the transcription of RUNX2 during osteoblast differentiation [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

The Effect of Strontium-Substituted Hydroxyapatite Nanofibrous Matrix on Osteoblast Proliferation and Differentiation

et al. 2021

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Natural bone tissue consists primarily of bioapatite and collagen. Synthetic hydroxyapatite (HA) possesses good biocompatibility, bioactivity, and osteoconductivity due to its chemical and biological similarity to bioapatite. Hence, HA has been widely used as a bone graft, cell carrier and drug/gene delivery carrier. Moreover, strontium-substituted hydroxyapatite (SrHA) can enhance osteogenic differentiation and inhibit adipogenic differentiation of mesenchymal stem cells. Hence, SrHA has the potential to be used as a bone graft for bone regeneration. It is widely accepted that cell adhesion and most cellular activities are sensitive to the topography and molecular composition of the matrix. Electrospun polymer or polymer-bioceramic composite nanofibers have been demonstrated to enhance osteoblast differentiation. However, to date, no studies have investigated the effect of nanofibrous bioceramic matrices on osteoblasts. In this study, hydroxyapatite nanofiber (HANF) and strontium-substituted hydroxyapatite nanofiber (SrHANF) matrices were fabricated by electrospinning. The effect of the HANF components on MG63 osteoblast-like cells was evaluated by cell morphology, proliferation, alkaline phosphatase activity (ALP) and gene expression levels of RUNX2, COLI, OCN and BSP. The results showed that MG63 osteoblast-like cells exhibited higher ALP and gene expression levels of RUNX2, COLI, BSP and OCN on the SrHANF matrix than the HANF matrix. Hence, SrHANFs could enhance the differentiation of MG63 osteoblast-like cells.

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Section: Resultsmentioning

confidence: 99%

The Effect of Strontium-Substituted Hydroxyapatite Nanofibrous Matrix on Osteoblast Proliferation and Differentiation

et al. 2021

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“…38 The activation of CaSR can elevate the concentration of intracellular second messengers DAG and IP3, and then the ERK1/2 cascade pathway was triggered. 39 Osteogenic related protein was synthesized, meanwhile, the receptor activator of NF-κb ligand (RANKL) was inhibited. 28 All these biological behaviors resulted in the promotion of osteoblasts proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have demonstrated that strontium was somehow like calcium, which can activate the calcium‐sensing receptor (CaSR) 38 . The activation of CaSR can elevate the concentration of intracellular second messengers DAG and IP3, and then the ERK1/2 cascade pathway was triggered 39 . Osteogenic related protein was synthesized, meanwhile, the receptor activator of NF‐κb ligand (RANKL) was inhibited 28 .…”

Section: Discussionmentioning

confidence: 99%

A novel porous titanium with engineered surface for bone defect repair in load‐bearing position

Liu,

Wang,

et al. 2024

J Biomedical Materials Res

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Porous titanium exhibits low elastic modulus and porous structure is thought to be a promising implant in bone defect repair. However, the bioinert and low mechanical strength of porous titanium have limited its clinical application, especially in load‐bearing bone defect repair. Our previous study has reported an infiltration casting and acid corrosion (IC‐AC) method to fabricate a novel porous titanium (pTi) with 40% porosity and 0.4 mm pore diameter, which exerts mechanical property matching with cortical bone and interconnected channels. In this study, we introduced a nanoporous coating and incorporated an osteogenic element strontium (Sr) on the surface of porous titanium (named as Sr‐micro arch oxidation [MAO]) to improve the osteogenic ability of the pTi by MAO. Better biocompatibility of Sr‐MAO was verified by cell adhesion experiment and cell counting kit‐8 (CCK‐8) test. The in vitro osteogenic‐related tests such as immunofluorescence staining, alkaline phosphatase staining and real‐time polymerase chain reaction (RT‐PCR) demonstrated better osteogenic ability of Sr‐MAO. Femoral bone defect repair model was employed to evaluate the osseointegration of samples in vivo. Results of micro‐CT scanning, sequential fluorochrome labeling and Van Gieson staining suggested that Sr‐MAO showed better in vivo osteogenic ability than other groups. Taking results of both in vitro and in vivo experiment together, this study indicated the Sr‐MAO porous titanium could be a promising implant load‐bearing bone defect.

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“…And studies have shown that this ion inhibits apoptosis, which occurs through the ATK pathway, in addition to encouraging adherence, and the differentiation of osteoblasts that promote the deposition of a new cellular matrix through the α2 and β1 integrin pathways [85,86]. It can also positively regulate the expression of osteogenic genes, such as ALP, OCN and BSP, through the activation of mitogen-activated protein kinase (MAPK) signaling and the phosphorylation of ERK1/2 [88]. In osteoclasts, through the expression of the SOST gene, Sr 2+ stimulates the production of sclerostin, which has the function of inhibiting osteogenesis and increasing bone mass [85,86].…”

Section: Bioactive Glasses Containing Strontiummentioning

confidence: 99%

Influence of Strontium on the Biological Behavior of Bioactive Glasses for Bone Regeneration

Silva,

Gomes,

Victor

et al. 2023

Materials

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Bioactive glasses (BGs) can potentially be applied in biomedicine, mainly for bone repair and replacement, given their unique ability to connect to natural bone tissue and stimulate bone regeneration. Since their discovery, several glass compositions have been developed to improve the properties and clinical abilities of traditional bioactive glass. Different inorganic ions, such as strontium (Sr2+), have been incorporated in BG due to their ability to perform therapeutic functions. Sr2+ has been gaining prominence due to its ability to stimulate osteogenesis, providing an appropriate environment to improve bone regeneration, in addition to its antibacterial potential. However, as there are still points in the literature that are not well consolidated, such as the influence of ionic concentrations and the BG production technique, this review aims to collect information on the state of the art of the biological behavior of BGs containing Sr2+. It also aims to gather data on different types of BGs doped with different concentrations of Sr2+, and to highlight the manufacturing techniques used in order to analyze the influence of the incorporation of this ion for bone regeneration purposes.

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Strontium Ameliorates Glucocorticoid Inhibition of Osteogenesis Via the ERK Signaling Pathway

Cited by 14 publications

References 45 publications

The Effect of Strontium-Substituted Hydroxyapatite Nanofibrous Matrix on Osteoblast Proliferation and Differentiation

The Effect of Strontium-Substituted Hydroxyapatite Nanofibrous Matrix on Osteoblast Proliferation and Differentiation

A novel porous titanium with engineered surface for bone defect repair in load‐bearing position

Influence of Strontium on the Biological Behavior of Bioactive Glasses for Bone Regeneration

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