Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis

Abstract

The heterogeneous stromal vascular fraction (SVF), containing adipose‐derived stem/progenitor cells (ASCs), can be easily isolated through enzymatic digestion of aspirated adipose tissue. In clinical settings, however, strict control of technical procedures according to standard operating procedures and validation of cell‐processing conditions are required. Therefore, we evaluated the efficiency and reliability of an automated system for SVF isolation from adipose tissue. SVF cells, freshly isolated using the automated procedure, showed comparable number and viability to those from manual isolation. Flow cytometric analysis confirmed an SVF cell composition profile similar to that after manual isolation. In addition, the ASC yield after 1 week in culture was also not significantly different between the two groups. Our clinical study, in which SVF cells isolated with the automated system were transplanted with aspirated fat tissue for soft tissue augmentation/reconstruction in 42 patients, showed satisfactory outcomes with no serious side‐effects. Taken together, our results suggested that the automated isolation system is as reliable a method as manual isolation and may also be useful in clinical settings. Automated isolation is expected to enable cell‐based clinical trials in small facilities with an aseptic room, without the necessity of a good manufacturing practice‐level cell processing area. Copyright © 2012 John Wiley & Sons, Ltd.

The Celution®System: Automated Processing of Adipose-Derived Regenerative Cells in a Functionally Closed System

“…Similar nonheadto-head data have been published from a different automated system manufactured by Tissue Genesis, Inc. (Honolulu, HI). 29 Flow cytometry data in the publication demonstrate that a substantial fraction of the cells express the CD34 cell surface marker protein indicating that the population isolated contains SVF cells. However, this device is limited to a maximum processing volume of 60 mL of tissue and prepares the cells in a large final volume (35 mL), which required the authors to perform an additional, manual centrifugation step to concentrate the cells before clinical use.…”

“…12,16 Recently, the United States Government has awarded Cytori Therapeutics, Inc., the developer of the Celution System, with a large research contract for evaluation of the system for potential use in patients with acute radiation exposure with concomitant thermal burn injury. 29 Specifically, the Biomedical Advanced Research and Development Authority (BARDA; a branch of the U.S. Department of Health and Human Services) awarded Cytori a research contract valued at up to US$106 million over 5 years. This contract will support preclinical and clinical research and device development related to use of the Celution System in patients with concomitant thermal burn injury and radiation exposure following a mass casualty event.…”

Stromal vascular stem cell treatment decreases muscle fibrosis following chronic rotator cuff tear

“…This procedure was approved by the University of Michigan Medical School IRB, and conducted based on previously described techniques [10, 11]. Briefly, an Icellator system (Tissue Genesis, Honolulu, HI, USA) and Adipase (Tissue Genesis) were used to gently digest and isolate the SVFCs according to the manufacturers protocols from approximately 60cc of subcutaneous lipoaspirate obtained from a patient undergoing a cosmetic procedure.…”

“…The concentration of viable cells was measured using a NucleoCounter (ChemoMetec, Davis, CA, USA), and greater than 90% of the isolated cells were determined to be viable. Previous studies have demonstrated that the stromal vascular fraction of cells obtained with the Icellator system is highly enriched with CD31 − /CD34 + /CD45 − adipose derived MSCs [10]. …”

Adipose stem cell-based regenerative medicine for reversal of diabetic hyperglycemia

“…Recently, adult stem cells from bone marrow, umbilical cord blood, pancreatic duct, periosteum, and adipose tissue have shown a capacity to differentiate into insulin-producing cells [32][33][34][35][36][37][38][39][40][41][42][43] . Among the many tissue sources for adult stem cells, adipose tissue is particularly attractive based on its stem cell abundance and ease of tissue procurement through a minimally invasive and relatively inexpensive procedure [44][45][46][47][48] . Mesenchymal stem cells from bone marrow and adipose tissue share similar cell populations, along with cell characteristics [49][50][51] .…”