Stromal p16 Overexpression in Gastric-type Mucinous Carcinoma of the Uterine Cervix

Chung, Taek; Im, Sung Il; Na, Kiyong; Kim, Geon; Jeong, Young In; Kim, Youn Wha; Kim, Hyunsoo

doi:10.21873/anticanres.12627

Cited by 17 publications

(21 citation statements)

References 26 publications

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“…Sections (4-μm thick) cut from formalin-fixed, paraffin-embedded tissue blocks were placed onto Superfrost Plus glass slides (Thermo Fisher Scientific, Waltham, MA, USA). Thereafter, the sections were deparaffinized in xylene, rehydrated through a series of graded alcohols and immunohistochemically stained using an automated Ventana Benchmark XT (Ventana Medical Systems Inc.) as described by the manufacturer (7)(8)(9)(10)(11)(12)(13). Antigen was retrieved using CC1 Cell Conditioning Solution (Ventana Medical Systems Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…According to the recommendation of the LAST Standardization Project (6), the immunostaining profile of p16 was interpreted as block positive when p16 expression was horizontally continuous and strong, with nuclear or nuclear plus cytoplasmic staining. All other p16 immunostaining profiles, described as focal nuclear, wispy, bloblike, puddled, or scattered cytoplasmic staining were interpreted as patchy positive (9,(14)(15)(16)(17)(18)(19). in vivo 34: 2613-2621 (2020)…”

Section: Methodsmentioning

confidence: 99%

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Clinicopathological Characteristics of Squamous Cell Carcinoma and High-grade Squamous Intraepithelial Lesions Involving Endocervical Polyps

Park

Kim

Koh

et al. 2020

In Vivo

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Background/Aim: To investigate the clinicopathological characteristics of high-grade squamous intraepithelial lesions (HSILs) and squamous cell carcinomas (SCCs) involving endocervical polyps (ECPs). Patients and Methods: We collected the endocervical polypectomy cases and performed pathological examination and cytohistological correlation. Results: During a period of 12 years, 21 (1.1%) HSILs and two (0.1%) SCCs involving ECPs were identified in 1,905 cases. Twelve (63.1%) of the 19 cases were cytohistologically concordant. In five HSILs and one SCC with polypectomy margin involvement, residual HSIL was identified in conization or hysterectomy specimens. Furthermore, in two HSIL patients and one SCC patient with negative polypectomy margins, residual HSILs were found in the conization specimens. Conclusion: The prevalence of HSIL and SCC involving ECP in our cohort was similar to the rates found in previous studies. The presence of residual HSIL in nonpolypoid cervical tissue regardless of the polypectomy margin involvement suggests that conization or hysterectomy is needed for diagnostic or treatment purposes. Endocervical polyps (ECPs) are the most common benign neoplasms of the uterine cervix that characteristically arise in the endocervical canal (1). They can occur over a wide age range, but about 90% of patients are aged >40 years (2). The reported prevalence of ECP is 1.5-10%, and its pathogenesis remains unknown. Focal reactive epithelial and stromal hyperplasia associated with repeated episodes of inflammation, an abnormal local response to increased estrogen levels, or local congestion of cervical stromal blood vessels might be involved in the development of ECP (1, 3). Approximately 60% of ECP patients is asymptomatic and discovered incidentally during routine gynecologic examinations, whereas symptomatic ECPs manifest as menorrhagia, metrorrhagia, postcoital bleeding, postmenopausal bleeding, vaginal discharge, or leukorrhea (2, 4). Even though the reported prevalence of malignancies arising from ECP is 0.1-0.5%, a thorough literature review revealed no information about malignancies involving ECP in Asian women (2, 3). We recently experienced several patients with high-grade squamous intraepithelial lesions (HSIL) and squamous cell carcinoma (SCC) involving ECP, which initiated a comprehensive review of our archival 2613 This article is freely accessible online.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Clinicopathological Characteristics of Squamous Cell Carcinoma and High-grade Squamous Intraepithelial Lesions Involving Endocervical Polyps

Park

Kim

Koh

et al. 2020

In Vivo

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“…The slices were then deparaffinized and rehydrated with a xylene and alcohol solution. Immunostaining was performed using an automatic instrument [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. After antigen retrieval, the slices were incubated with primary antibodies including p16 (prediluted, clone E6H4, Ventana Medical Systems, Oro Valley, AZ, USA) and Ki-67 (1:200, clone MIB-1, Dako, Glostrup, Denmark).…”

Section: Methodsmentioning

confidence: 99%

Clinicopathological Characteristics of Pleomorphic High-Grade Squamous Intraepithelial Lesion of the Uterine Cervix: A Single-Institutional Series of 31 Cases

Kim

Choi

et al. 2020

Diagnostics

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We investigated the clinicopathological characteristics of 31 cases of pleomorphic high-grade squamous intraepithelial lesions (PHSIL) of the uterine cervix. We reviewed electronic medical records and all available slides to collect clinical and pathological information. PHSILs were histologically characterized by significant nuclear enlargement, marked pleomorphism, hyperchromasia, increased mitotic activity, and frequent atypical mitoses. In the majority of cases (24/31; 77.4%), this striking nuclear atypia involved both the surface epithelium and the endocervical glands. In the remaining seven cases, pleomorphic cells were observed in the surface epithelium only. PHSILs involving both the surface epithelium and glands showed higher mitotic counts and Ki-67 labelling indices than the surface-only PHSILs. Invasive squamous cell carcinoma was present in only one case (3.2%), and none developed recurrent disease. Our observations of striking nuclear atypia in cases of HSIL did not indicate increased aggressiveness. Further investigations are required for confirmation of our data in larger cohorts.

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“…The 4 µm-thick, FFPE slices were de-paraffinized and rehydrated using a xylene and alcohol solution. Immunostaining was performed using automated instruments (Ventana Benchmark XT (Ventana Medical Systems) and or Dako Omnis (Dako, Carpinteria, CA, USA)) [1,[18][19][20][21][22][23][24][25][26]. After antigen retrieval, the slices were incubated with primary antibodies including pan-cytokeratin (pan-CK; 1:600, clone AE1/AE3, Dako), CK7 (Dako, 1:100, clone OV-TL 12/30, Dako), CK20 (1:100, clone Ks20.8, Dako), caudal type homeobox 2 (CDX2; 1:400, clone EPR2764Y, Cell Marque, Rocklin, CA, USA), paired box 8 (PAX8; 1:50, polyclonal, Cell Marque), estrogen receptor (ER; 1:150, clone 6F11, Novocastra, Leica Biosystems, Newcastle Upon Tyne, UK), progesterone receptor (PR; 1:100, clone 16, Novocastra), p53 (1:300, clone DO-7, Novocastra), p16 (prediluted, clone E6H4, Ventana Medical Systems), p63 (1:50, clone 4A4, Dako), and GATA-binding protein 3 (GATA3; 1:150, clone L50-823, Cell Marque).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Endometrium-Limited Metastasis of Extragenital Malignancies: A Challenge in the Diagnosis of Endometrial Curettage Specimens

Choi

Joo

et al. 2020

Diagnostics

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Malignancies of extragenital origin very rarely metastasize to the uterine body. Endometrium-limited metastases may pose diagnostic challenges in endometrial curettage specimens as they may be misdiagnosed as primary endometrial tumors. We investigated the clinicopathological characteristics of seven cases with endometrial-limited metastases from carcinomas of the nasopharynx (n = 1), breast (n = 2), colon (n = 2), stomach (n = 1), and appendix (n = 1). The patients’ ages ranged from 36 to 71 (mean: 55.4) years. None of the patients had a remarkable gynecological history, and the presenting sign in all cases was abnormal uterine bleeding. Although myometrial involvement was absent, multiple metastases were already present in extrauterine locations such as the lung, liver, bone, abdominopelvic peritoneum, and omentum. All patients underwent ultrasonographic examination prior to endometrial curettage. The histologies of the endometrial metastases identified from the curettage specimens were identical to those of the corresponding primary tumors. Ancillary tests including immunostaining and Epstein–Barr virus-encoded RNA in situ hybridization confirmed the extragenital origin. Endometrium-limited metastases from extragenital malignancies are extremely rare. They present with abnormal vaginal bleeding and mimic endometrial carcinomas of endometrioid or poorly differentiated types. Since their clinical presentations and histological features are similar to those of primary endometrial tumors, pathologists should consider the possibility of metastases while evaluating endometrial curettage specimens obtained from patients with a history of extragenital malignancies.

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Stromal p16 Overexpression in Gastric-type Mucinous Carcinoma of the Uterine Cervix

Cited by 17 publications

References 26 publications

Clinicopathological Characteristics of Squamous Cell Carcinoma and High-grade Squamous Intraepithelial Lesions Involving Endocervical Polyps

Clinicopathological Characteristics of Squamous Cell Carcinoma and High-grade Squamous Intraepithelial Lesions Involving Endocervical Polyps

Clinicopathological Characteristics of Pleomorphic High-Grade Squamous Intraepithelial Lesion of the Uterine Cervix: A Single-Institutional Series of 31 Cases

Endometrium-Limited Metastasis of Extragenital Malignancies: A Challenge in the Diagnosis of Endometrial Curettage Specimens

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