“…Briefly, 4-μm-thick, formalin-fixed, paraffinembedded slices were deparaffinized and rehydrated using a xylene and alcohol solution. Immunostaining was performed using the Bond Polymer Intense Detection System (Vision Biosystems, Mount Waverly, Victoria, Australia) (13)(14)(15)(16)(17)(18)(19). After antigen retrieval, the slices were incubated with primary antibodies including estrogen receptor (ER; 1:150, clone 6F11, Novocastra, Leica Biosystems, Newcastle Upon Tyne, UK), progesterone receptor (PR; 1:100, clone 16, Novocastra), p53 (1:300, clone DO-7, Novocastra), p16 (prediluted, clone E6H4, Ventana Medical Systems, Oro Valley, AZ, USA), neural cell adhesion molecule (CD56, 1:200, clone CD564, Novocastra), chromogranin A (1:800, clone DAK-A3, Dako, Agilent Technologies, Inc., Santa Clara, CA, USA), synaptophysin (1:400, clone DAKO-SYNAP, Dako), and Wilms tumor 1 (WT1, 1:800, clone 6F-H2, Cell Marque, Rocklin, CA, USA).…”