Strikingly Different Angiogenic Properties of Endothelial Progenitor Cell Subpopulations

Sieveking, Daniel; Buckle, Andrew; Celermajer, David S.; Ng, M.

doi:10.1016/j.jacc.2007.09.059

Cited by 316 publications

(134 citation statements)

References 24 publications

(37 reference statements)

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…The early EPC population, also referred to as early outgrowth cells, is derived after 7 to 10 days in culture, whereas the late EPCs arise after 14 to 21 days in culture. Early EPCs possess a lower expression of endothelial lineage markers and secrete more vasoreparative factors when compared with late EPCs 11, 13, 14. In vivo, circulating EPC numbers and the function of EPCs are decreased in patients with cardiovascular disease or diabetes mellitus 15, 16, 17, 18.…”

mentioning

confidence: 99%

Loss of the Liver X Receptors Disrupts the Balance of Hematopoietic Populations, With Detrimental Effects on Endothelial Progenitor Cells

Rasheed

Tsai

Cummins

2018

JAHA

View full text Add to dashboard Cite

BackgroundThe liver X receptors (LXRs; α/β) are nuclear receptors known to regulate cholesterol homeostasis and the production of select hematopoietic populations. The objective of this study was to determine the importance of LXRs and a high‐fat high‐cholesterol diet on global hematopoiesis, with special emphasis on endothelial progenitor cells (EPCs), a vasoreparative cell type that is derived from bone marrow hematopoietic stem cells.Methods and ResultsWild‐type and LXR double‐knockout (Lxrαβ−/−) mice were fed a Western diet (WD) to increase plasma cholesterol levels. In WD‐fed Lxrαβ−/− mice, flow cytometry and complete blood cell counts revealed that hematopoietic stem cells, a myeloid progenitor, and mature circulating myeloid cells were increased; EPC numbers were significantly decreased. Hematopoietic stem cells from WD‐fed Lxrαβ−/− mice showed increased cholesterol content, along with increased myeloid colony formation compared with chow‐fed mice. In contrast, EPCs from WD‐fed Lxrαβ−/− mice also demonstrated increased cellular cholesterol content that was associated with greater expression of the endothelial lineage markers Cd144 and Vegfr2, suggesting accelerated differentiation of the EPCs. Treatment of human umbilical vein endothelial cells with conditioned medium collected from these EPCs increased THP‐1 monocyte adhesion. Increased monocyte adhesion to conditioned medium–treated endothelial cells was recapitulated with conditioned medium from Lxrαβ−/− EPCs treated with cholesterol ex vivo, suggesting cholesterol is the main component of the WD inducing EPC dysfunction.Conclusions LXRs are crucial for maintaining the balance of hematopoietic cells in a hypercholesterolemic environment and for mitigating the negative effects of cholesterol on EPC differentiation/secretome changes that promote monocyte‐endothelial adhesion.

show abstract

mentioning

confidence: 99%

Loss of the Liver X Receptors Disrupts the Balance of Hematopoietic Populations, With Detrimental Effects on Endothelial Progenitor Cells

Rasheed

Tsai

Cummins

2018

JAHA

View full text Add to dashboard Cite

show abstract

“…It is well known that EPCs consist of two different subpopulations, termed early and late EPCs, which are phenotypically and morphologically distinct. Recently, it has been shown that their role in vascular network formation is also strikingly different [48,49]. It is presumed that the endothelial cells expanded in the present study are reasonably late outgrowth ECs, according to the time at which they appear in culture and their cobblestone morphology [29,30].…”

Section: Discussionmentioning

confidence: 56%

Capability of human umbilical cord blood progenitor-derived endothelial cells to form an efficient lining on a polyester vascular graft in vitro

et al. 2009

View full text Add to dashboard Cite

“…Interestingly, further studies associated the presence/absence of surface markers with specific functionalities of EPCs, like vascular homing and repair competence, incorporation into the damaged endothelium, and in vivo vessel formation (Asahara et al, 1997;Becher et al, 2010;Friedrich et al, 2006;Hur et al, 2004;Sieveking et al, 2008).…”

Section: The Role Of Endothelial Progenitor Cells In Vascular Integritymentioning

confidence: 99%

Infections and cardiovascular disease: is Bartonella henselae contributing to this matter?

Salvatore

Zullo

Sommese

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Infections and cardiovascular disease: is Bartonella henselae contributing to this matter? Cardiovascular disease is still the major cause of death worldwide despite the remarkable progress in its prevention and treatment. Endothelial progenitor cells (EPCs) have recently emerged as key players of vascular repair and regenerative medicine applied to cardiovascular disease. A large amount of effort has been put into discovering the factors that could aid or impair the number and function of EPCs, and also into characterizing these cells at the molecular level in order to facilitate their therapeutic applications in vascular disease. Interestingly, the major cardiovascular risk factors have been associated with reduced number and function of EPCs. The bacterial contribution to cardiovascular disease represents a long-standing controversy. The discovery that Bartonella henselae can infect and damage EPCs revitalizes the enduring debate about the microbiological contribution to atherosclerosis, thus allowing the hypothesis that this infection could impair the cardiovascular regenerative potential and increase the risk for cardiovascular disease. In this review, we summarize the rationale suggesting that Bartonella henselae could favour atherogenesis by infecting and damaging EPCs, thus reducing their vascular repair potential. These mechanisms suggest a novel link between communicable and non-communicable human diseases, and put forward the possibility that Bartonella henselae could enhance the susceptibility and worsen the prognosis in cardiovascular disease.

show abstract

Strikingly Different Angiogenic Properties of Endothelial Progenitor Cell Subpopulations

Cited by 316 publications

References 24 publications

Loss of the Liver X Receptors Disrupts the Balance of Hematopoietic Populations, With Detrimental Effects on Endothelial Progenitor Cells

Loss of the Liver X Receptors Disrupts the Balance of Hematopoietic Populations, With Detrimental Effects on Endothelial Progenitor Cells

Capability of human umbilical cord blood progenitor-derived endothelial cells to form an efficient lining on a polyester vascular graft in vitro

Infections and cardiovascular disease: is Bartonella henselae contributing to this matter?

Contact Info

Product

Resources

About