Capability of human umbilical cord blood progenitor-derived endothelial cells to form an efficient lining on a polyester vascular graft in vitro

In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

“…To support cells seeded in vitro, a variety of cell types, materials, fabrication techniques, and bioreactors have been used to provide a mechanical and biological environment for the development of tissue-engineered vascular grafts (TEVGs). [4][5][6][7][8] In this study, we focus on seeding, proliferating, and differentiating early endothelial progenitor cells (EPCs) on a biodegradable vascular graft within a tubular perfusion system (TPS) bioreactor.…”

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

“…The cells adhere, are maintained, but do not proliferate until day 9. Nevertheless, the application of flow for 6 h restores the endothelial monolayer [41]. In our study, the bovine EC significantly increased in number on both pure PET and proteinmodified PET surfaces during 7 day culture even under static conditions.…”

“…Bérard et al [41] tested the lining of knitted PET prostheses commercially impregnated with type I and type III bovine collagen (i.e. unmodified) with human umbilical cord blood progenitor-derived EC in serum-free conditions.…”

Polyelectrolyte multilayer films promote human cord blood stem cells differentiation into mature endothelial cells exhibiting a stable phenotype

“…In conclusion, this EPCs source has recently brought new possibilities in vascular and tissue bioengineering [2,17]. In parallel, many applications of the PEMs have already been demonstrated [5,10,12], in favour of stem cells differentiation, but also for prosthesis coating.…”

“…Moreover, compared to peripheral blood, cord blood contains much more mononuclear cells, which is in favour of a quick EPCs expansion [14]. Umbilical cord bloodderived EPCs were successfully used in bio-engineering, in particular on heart valve leaflets [17] and on vessels [2].…”