<i>In Vitro</i> Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

Melchiorri, Anthony J.; Bracaglia, Laura G.; Kimerer, Lucas K.; Hibino, Narutoshi; Fisher, John P.

doi:10.1089/ten.tec.2015.0562

Cited by 70 publications

(52 citation statements)

References 47 publications

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“…Mahmoud et al showed that the EndMT can be induced under low shear stress (0.4 Pa) [133]. In our approach, the cells experienced a wall shear stress of about 0.03 Pa, which is slightly lower than a venous wall shear stress (0.06 Pa) [134]. In silico simulations of our dynamic bioreactor culture confirmed laminar flow conditions along a large part of the vascular wall using the applied parameters.…”

Section: Discussionmentioning

confidence: 50%

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

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Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.

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Section: Discussionmentioning

confidence: 50%

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

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“…When in vivo, VSMCs are continuously exposed to a maximum cyclic strain (ε circ ) of around 10%, while ECs and their progenitors (EPCs) are subjected to a fluid wall shear stress (WSS) in the range of 5-20 and 0.1-2.5 dyne/ cm 2 , respectively, due to pulsatile blood flow [5]. Cyclic strain aligns VSMCs in a circumferential direction and preserves their contractile phenotype; WSS addresses ECs to dispose longitudinally to the fluid direction and to keep their quiescent state and anti-inflammatory function, therefore creating a functional endothelial monolayer [15][16][17][18]. Thus, pulsatile perfusion is often adopted as an in vitro mechanical stimulus, using perfusion bioreactors on tubular scaffolds with seeded VSMCs in the external side and ECs in the lumen.…”

Section: Introductionmentioning

confidence: 99%

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi

Pacheco

Formenti

et al. 2019

Materials Science and Engineering: C

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Aiming to perfuse porous tubular scaffolds for vascular tissue engineering (VTE) with controlled flow rate, prevention of leakage through the scaffold lumen is required. A gel coating made of 8% w/v alginate and 6% w/v gelatin functionalized with fibronectin was produced using a custom-made bioreactor-based method. Different volumetric proportions of alginate and gelatin were tested (50/50, 70/30, and 90/10). Gel swelling and stability, and rheological, and uniaxial tensile tests reveal superior resistance to the aggressive biochemical microenvironment, and their ability to withstand physiological deformations (~10%) and wall shear stresses (5-20 dyne/cm 2). These are prerequisites to maintain the physiologic phenotypes of vascular smooth muscle cells and endothelial cells (ECs), mimicking blood vessels microenvironment. Gels can induce ECs proliferation and colonization, especially in the presence of fibronectin and higher percentages of gelatin. The custom-designed bioreactor enables the development of reproducible and homogeneous tubular gel coating. The permeability tests show the effectiveness of tubular scaffolds coated with 70/30 alginate/gelatin gel to occlude wadding pores, and therefore prevent leakages. The synthesized double-layered tubular scaffolds coated with alginate/gelatin gel and fibronectin represent both promising substrate for ECs and effective leakproof scaffolds, when subjected to pulsatile perfusion, for VTE applications.

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“…Bioreactors are also popular tools for developing vascularized tissue constructs, as flow‐induced shear stress is known to promote vascularization . Consequently, the presence of dynamic flow is key to developing a full‐thickness skin tissue with the required degree of vasculature for rapid in vivo integration.…”

Section: Advanced Tissue Engineering Approaches To Regenerate Skinmentioning

confidence: 99%

Current and Future Perspectives on Skin Tissue Engineering: Key Features of Biomedical Research, Translational Assessment, and Clinical Application

Navarro

Coburn

et al. 2019

Adv Healthcare Materials

Self Cite

144

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The skin is responsible for several important physiological functions and has enormous clinical significance in wound healing. Tissue engineered substitutes may be used in patients suffering from skin injuries to support regeneration of the epidermis, dermis, or both. Skin substitutes are also gaining traction in the cosmetics and pharmaceutical industries as alternatives to animal models for product testing. Recent biomedical advances, ranging from cellular‐level therapies such as mesenchymal stem cell or growth factor delivery, to large‐scale biofabrication techniques including 3D printing, have enabled the implementation of unique strategies and novel biomaterials to recapitulate the biological, architectural, and functional complexity of native skin. This progress report highlights some of the latest approaches to skin regeneration and biofabrication using tissue engineering techniques. Current challenges in fabricating multilayered skin are addressed, and perspectives on efforts and strategies to meet those limitations are provided. Commercially available skin substitute technologies are also examined, and strategies to recapitulate native physiology, the role of regulatory agencies in supporting translation, as well as current clinical needs, are reviewed. By considering each of these perspectives while moving from bench to bedside, tissue engineering may be leveraged to create improved skin substitutes for both in vitro testing and clinical applications.

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In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor

Cited by 70 publications

References 47 publications

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Current and Future Perspectives on Skin Tissue Engineering: Key Features of Biomedical Research, Translational Assessment, and Clinical Application

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