Stretch‐mediated responses of osteoblast‐like cells cultured on titanium‐coated substrates <i>in vitro</i>

Walboomers, X. Frank; Habraken, Wouter J. E. M.; Feddes, B.; Winter, Lukas; Bumgardner, Joel D.; Jansen, John A.

doi:10.1002/jbm.a.20127

Cited by 20 publications

(21 citation statements)

References 24 publications

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“…MG63 (29) and HOS-TE85 (30) cells isolated from human osteosarcomas, UMR-106-01, UMR-106-06, ROS17/2.8; MSK cells isolated from rat osteosarcomas; and MC3T3-E1 cells isolated from the calvaria of an embryo/fetus mouse have also been established as cell lines in culture. In this study, osteoblast-like cells from rat femur were chosen because those cells have long been recognized as a possible source of osteoprogenitor cells both in vivo and in vitro (17,(24)(25)(26)(27)(28)(31)(32)(33)(34). However, there is little known about cell responses under centrifugal force in terms of proliferation, differentiation and homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

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Section: Discussionmentioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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“…Centrifuge setting ranges were 10, 25 or 50 g for two different time periods: 4 or 24 hours. [Wang et al, 1995;Neidlinger-Wilke et al, 2002;Walboomers et al, 2004]. Directly at the end of each time period, the RDF cell layers were washed three times with PBS, fixed and prepared for further analysis.…”

Section: Cell Culturementioning

confidence: 99%

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

Loesberg

Walboomers

Loon

et al. 2006

Cell Motil. Cytoskeleton

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This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and micro-grooved substrata (groove depth: 1 mum, width: 1, 2, 5, 10 microm), which undergo artificial hypergravity by centrifugation (10, 24 and 50 g; or 1 g control). The aim of the study was to clarify which of these parameters was more important to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell spreading and alignment. Confocal laser scanning microscopy visualised distribution of actin filaments and vinculin anchoring points through immunostaining. Finally, expression of collagen type I, fibronectin, and alpha(1)- and beta(1)-integrin were investigated by PCR. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata (control), cells spread out in a random fashion. The alignment of cells cultured on grooved surfaces increased with higher g-forces until a peak value at 25 g. An ANOVA was performed on the data, for all main parameters: topography, gravity force, and time. In this analysis, all parameters proved significant. In addition, most gene levels were reduced by hypergravity. Still, collagen type 1 and fibronectin are seemingly unaffected by time or force. From our data it is concluded that the fibroblasts primarily adjust their shape according to morphological environmental cues like substratum surface whilst a secondary, but significant, role is played by hypergravity forces.

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“…Previous studies already confirmed that the composition of thin magnetron sputtered titanium coatings is very similar to the surface composition of bulk titanium implants [9]. Rutherford backscattering spectroscopy and Xray photoelectron spectroscopy indicated that sputtered titanium coatings consist of titanium and oxygen in amounts of 1:1.…”

Section: Discussionmentioning

confidence: 64%

Evaluation of bone response to titanium-coated polymethyl methacrylate resin (PMMA) implants by X-ray tomography

Shalabi

Wolke

Cuijpers

et al. 2007

J Mater Sci: Mater Med

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High-resolution three-dimensional data about the bone response to oral implants can be obtained by using microfocus computer tomography. However, a disadvantage is that metallic implants cause streaking artifacts due to scattering of X-rays, which prevents an accurate evaluation of the interfacial bone-to-implant contact. It has been suggested that the use of thin titanium coatings deposited on polymeric implants can offer an alternative option for analyzing bone contact using micro-CT imaging. Consequently, the aim of the current study was to investigate bone behavior to titanium-coated polymethylmethacrylate (PMMA) implants by micro-CT and histological evaluation. For the experiment titanium-coated PMMA implants were used. The implants had a machined threaded appearance and were provided with a 400-500 nm thick titanium coating. The implants were inserted in the right or left tibia of 10 goats. After an implantation period of 12 weeks the implants were retrieved and prepared for micro-computer tomography (lCT), light microscopy, and X-ray microanalysis. The micro-CT showed that the screwthreads and typical implant configuration were well maintained through the installation procedure. Overall, histological responses showed that the titanium-coated implants were well tolerated and caused no atypical tissue response. In addition, the bone was seen in direct contact with the titanium-coated layer. The X-ray microanalysis results confirmed the light microscopical data. In conclusion, the obtained results proof the final use of titanium-coated PMMA implants for evaluation of the bone-implant response using lCT. However, this study also confirms that for a proper analysis of the bone-implant interface the additional use of microscopical techniques is still required.

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Stretch‐mediated responses of osteoblast‐like cells cultured on titanium‐coated substrates in vitro

Cited by 20 publications

References 24 publications

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

Evaluation of bone response to titanium-coated polymethyl methacrylate resin (PMMA) implants by X-ray tomography

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