Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Abstract

Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

<b>Response to light compressive force in human cementoblasts </b><i><b>in </b></i><i><b>vitro </b></i>

“…Our results showed no significant differences in cell numbers between the compressed and control groups. Previous studies reported slower proliferation of osteoblast-like cells on weighted glass cylinders and faster proliferation using centrifugal force (17,25). These differences may be caused by the application of compressive force or the characteristics of HCEM.…”

“…Various methods have been developed for the application of compressive forces in vitro, including weighted glass cylinders (18), hydrostatic pressure (2), the release of a pre-stretched membrane (16), and centrifugal force (25,28). Cell damage such as apoptosis (13) must also be considered during the application of force.…”

Influence of Mechanical Force on Bone Matrix Proteins in Ovariectomised Mice and Osteoblast-like MC3T3-E1 Cells

“…MC3T3-E1 cells were seeded at 5×10 5 cells/well in 24-well plates in triplicate. After 24 h, they were burdened by mechanical stress by use of a centrifuge (CF-7D; Hitachi Koki Co., Ltd., Tokyo, Japan) (24,25). The cells were divided into control (no centrifugation), and groups centrifuged at 16 ×g) or 64 ×g).…”

Effect of mechanical loading on cultured osteogenic cells derived from different stages of bone wound healing in rats: experimental models for immediate or early implantation

“…The answer will likely depend upon factors such as implant geometry and surface texture, the loading applied to the implant, the initial anchorage of the implant in bone, and the quality and quantity of the surrounding bone. Naito et al obtained the results showing that adequate mechanical force promotes the cell proliferation and early differentiation of osteoblastic cells (38). Leucht et al reported that when implant micromotion creates effective strains, osseointegration occurs (39).…”