Stretch‐driven microfluidic chip for nucleic acid detection

Abstract

Molecular diagnosis is an essential means to detect pathogens. The portable nucleic acid detection chip has excellent prospects in places where medical resources are scarce, and it is also of research interest in the field of microfluidic chips. Here, the article developed a new type of microfluidic chip for nucleic acid detection where stretching acts as the driving force. The sample entered the chip by applying capillary force. The strain valve was opened under the action of tensile force, and the spring pump generated the power to drive the fluid to flow to the detection chamber in a specific direction. The detection of coronavirus disease 2019 (COVID‐19) was realized on the chip. The RT‐LAMP amplification system was adopted to observe the liquid color in the detection chamber to decide whether the sample tested positive or negative qualitatively.

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

“…Researchers [ 108 ]. developed a new microfluidic chip for nucleic acid detection using stretching acts as the driving force.…”

Recent Advances, Opportunities, and Challenges in Developing Nucleic Acid Integrated Wearable Biosensors for Expanding the Capabilities of Wearable Technologies in Health Monitoring

“…Then, a mask is applied to the coated substrate and inserted into a mask aligner machine to make negative or positive patterns of microfluidic design. Using this process, a master mold for a three-layer microfluidic chamber was fabricated for nucleic acid detection [ 31 ]. The first and the third layer would encapsulate the microfluidics on the second layer and seal the channels.…”

“…This master mold was used with a mixture of Polydimethylsiloxane (PDMS) precursors and curing agents to develop the fluidics prototype. Another thin layer was fabricated to cover the microfluidic channel for reagent integration using an oxygen plasma treatment ( Figure 2 a) [ 31 ]. The silanol groups (-OH) on the surface of the PDMS layers are exposed more after plasma treatment, and once they are brought together with other PDMS layers, they create strong covalent (Si-O-Si) connections.…”

“…( a ) Microfluidics was patterned and assembled using three layers of PDMS (Reprinted with permission from Ref. [ 31 ] Copyright 2021, John Wiley and Sons). ( b ) Polyurethane microfluidic pattern produced by photolithography (Reprinted with permission from Ref.…”

Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications

“…For example, Ezgi Ahi et al fabricated a capillary-driven microfluidic chip with four continuous chambers for SERS-based hCG detection [ 83 ]. Li et al prepared a stretch-driven microfluidic channel with multiple wing structures for nucleic acid detection [ 84 ].…”