Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Li, Zheng; Xu, Xiaojian; Wang, Dou; Jiang, Xingyu

doi:10.1016/j.trac.2022.116871

Cited by 35 publications

(25 citation statements)

References 138 publications

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“…The small size and the high efficiency enable them to be well applied in the POC test . A microfluidic chip typically integrates a miniaturized flow pipeline, valves, reaction chambers, and a detector . Once a droplet of sample was added to the microfluidic chip, it would automatically flow through all components in the chip and generate a signal in the end.…”

Section: Portability Improving Strategiesmentioning

confidence: 99%

“…83 A microfluidic chip typically integrates a miniaturized flow pipeline, valves, reaction chambers, and a detector. 84 Once a droplet of sample was added to the microfluidic chip, it would automatically flow through all components in the chip and generate a signal in the end. And some well-designed microfluidic chips can even achieve simultaneous multiplexed detection.…”

Section: ■ Portability Improving Strategiesmentioning

confidence: 99%

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

Li,

Cheng

2023

ACS Sens.

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Point-of-care (POC) detection is getting more and more attention in many fields due to its accuracy and on-site test property. The CRISPR/Cas12a system is endowed with excellent sensitivity, target identification specificity, and signal amplification ability in biosensing because of its unique trans-cleavage ability. As a result, a lot of research has been made to develop CRISPR/Cas12a-based biosensors. In this review, we focused on signal readout strategies and summarized recent sensitivity-improving strategies in fluorescence, colorimetric, and electrochemical signaling. Then we introduced novel portability-improving strategies based on lateral flow assays (LFAs), microfluidic chips, simplified instruments, and one-pot design. In the end, we also provide our outlook for the future development of CRISPR/Cas12a biosensors.

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Section: Portability Improving Strategiesmentioning

confidence: 99%

Section: ■ Portability Improving Strategiesmentioning

confidence: 99%

Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

Li,

Cheng

2023

ACS Sens.

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“…There is an urgent need for more powerful diagnostic tools to improve testing capabilities. The outbreak of COVID-19 has greatly advanced the development of nucleic acid testing and extended its application to accurate and rapid molecular diagnosis of other pathogens. , Quantitative real-time PCR is the gold standard for nucleic acid detection. , The integration of PCR with point-of-care testing (POCT) is limited by the high dependence on external instruments such as thermal cyclers, high cost per test, reagent storage, the need for specialized personnel, and so on. − …”

Section: Introductionmentioning

confidence: 99%

Automated Microfluidic Nucleic Acid Detection Platform-Integrated RPA-T7-Cas13a for Pathogen Diagnosis

Hua

Xie

et al. 2023

Anal. Chem.

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There is a growing urgent need for point-of-care testing (POCT) devices that integrate sample pretreatment and nucleic acid detection in a rapid, economical, and non-laborintensive way. Here, we have developed an automated, portable nucleic acid detection system employing microfluidic chips integrating rotary valve-assisted sample pretreatment and recombinase polymerase amplification (RPA)-T7-Cas13a into one-step nucleic acid detection. The RPA and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a were integrated into a single-chamber reaction. As a validation model, we used this method to detect Group B streptococci (GBS) DNA and achieved a detection sensitivity of 8 copies/reaction, which is 6 times more sensitive than gold-standard polymerase chain reactions (PCRs). Dual specific recognition of RPA with CRISPR/Cas13a makes our method ultraspecific, with correct detection of Group B streptococci from 8 kinds of pathogenic bacteria. For the 16 positive and 24 negative clinical GBS samples, our assay achieved 100% accuracy compared to the PCR technique. The whole procedure can be automatically completed within 30 min, providing a more robust, sensitive, and accurate molecular diagnostic tool for POCT.

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“…Highly sensitive and specific nucleic acid detection is crucial for in vitro diagnosis of pathogenic microorganisms. 1,2 In recent years, the advancement of clustered regularly interspaced short palindromic repeat (CRISPR) technology has innovated the field of nucleic acid detection because it could sensitively discriminate single-nucleotide polymorphisms and possess outstanding signal amplification capacity. 3,4 For example, Cas12 is a type V CRISPR-associated protein which can precisely recognize the target DNA fragment and trigger a conformational change under the guidance of CRISPR RNA (crRNA), thereby sequentially activating its cis-and trans-cleavage activities.…”

Section: Introductionmentioning

confidence: 99%

Magnetofluid-integrated biosensors based on DNase-dead Cas12a for visual point-of-care testing of HIV-1 by an up and down chip

Huang,

Zhao,

Fang

et al. 2023

Lab Chip

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Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Cited by 35 publications

References 138 publications

Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

Automated Microfluidic Nucleic Acid Detection Platform-Integrated RPA-T7-Cas13a for Pathogen Diagnosis

Magnetofluid-integrated biosensors based on DNase-dead Cas12a for visual point-of-care testing of HIV-1 by an up and down chip

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