In this study, conventional long ramie fiber was stretch-broken into short fibers with lengths of 30 mm, 35 mm, 40 mm, 45 mm, and 50 mm. Then, these stretch-broken fibers were processed in a cotton spinning system. The results show that, compared to long ramie fibers processed in a conventional ramie spinning system, the stretch-broken fibers, with reasonable fiber length and high length uniformity, can be processed in a cotton spinning system with high efficiency and generally have better resultant yarn quality. For all of the stretch-broken yarns, the yarn processed from fiber with 40 mm length shows the best comprehensive performance.