Stressors alter intercellular communication and exosome profile of nasopharyngeal carcinoma cells

Harmati, Mária; Tarnai, Zsófia; Decsi, Gábor; Körmöndi, Sándor; Szegletes, Zsolt; Janovák, László; Dékány, Imre; Saydam, Okay; Gyukity-Sebestyen, Edina; Dobra, Gabriella; Nagy, István; Nagy, Katalin; Buzás, Krisztina

doi:10.1111/jop.12486

Cited by 41 publications

(40 citation statements)

References 34 publications

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“…The presence and potential role of extracellular vesicles especially exosomes in follicular fluid has been reported in mare [ 25 ], women [ 68 ] and cow [ 22 , 69 ]. The quality and quantity of these extracellular vesicles vary depending on the physiological status of the cells, from where they are released [ 68 , 70 , 71 ] and are highly triggered by various stresses factors including diseases, heat stress as well as oxidative stress [ 72 ]. In agreement with that, our resultes demonstrated that culture media containing granulosa cells exposed to stress contained higher concentration of exosomes compared to those released by granulosa cells under normal conditions ( Fig 7 ).…”

Section: Discussionmentioning

confidence: 99%

Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress

et al. 2017

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Various environmental insults including diseases, heat and oxidative stress could lead to abnormal growth, functions and apoptosis in granulosa cells during ovarian follicle growth and oocyte maturation. Despite the fact that cells exposed to oxidative stress are responding transcriptionally, the potential release of transcripts associated with oxidative stress response into extracellular space through exosomes is not yet determined. Therefore, here we aimed to investigate the effect of oxidative stress in bovine granulosa cells in vitro on the cellular and exosome mediated defense mechanisms. Bovine granulosa cells were aspirated from ovarian follicles and cultured in DMEM/F-12 Ham culture medium supplemented with 10% exosome-depleted fetal bovine serum. In the first experiment sub-confluent cells were treated with 5 μM H2O2 for 40 min to induce oxidative stress. Thereafter, cells were subjected to ROS and mitochondrial staining, cell proliferation and cell cycle assays. Furthermore, gene and protein expression analysis were performed in H2O2-challenged versus control group 24 hr post-treatment using qRT-PCR and immune blotting or immunocytochemistry assay, respectively. Moreover, exosomes were isolated from spent media using ultracentrifugation procedure, and subsequently used for RNA isolation and qRT-PCR. In the second experiment, exosomes released by granulosa cells under oxidative stress (StressExo) or those released by granulosa cells without oxidative stress (NormalExo) were co-incubated with bovine granulosa cells in vitro to proof the potential horizontal transfer of defense molecules from exosomes to granulosa cells and investigate any phenotype changes. Exposure of bovine granulosa cells to H2O2 induced the accumulation of ROS, reduced mitochondrial activity, increased expression of Nrf2 and its downstream antioxidant genes (both mRNA and protein), altered the cell cycle transitions and induced cellular apoptosis. Granulosa cells exposed to oxidative stress released exosomes enriched with mRNA of Nrf2 and candidate antioxidants. Subsequent co-incubation of StressExo with cultured granulosa cells could alter the relative abundance of cellular oxidative stress response molecules including Nrf2 and antioxidants CAT, PRDX1 and TXN1. The present study provide evidences that granulosa cells exposed to oxidative stress conditions react to stress by activating cascades of cellular antioxidant molecules which can also be released into extracellular environment through exosomes.

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Section: Discussionmentioning

confidence: 99%

Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress

et al. 2017

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“…Different kinds of exosomes have been isolated from the serum of NPC patients, such as EBV-related exosomes and pure nasopharyngeal carcinoma-derived exosomes 40 . These exosomes contain different contents and thus play differential roles in tumor-host crosstalk and modulate the immune response, angiogenesis, cell proliferation, cell-to-cell communication, and tumor invasion 41 , 42 .…”

Section: Nasopharyngeal Carcinoma and Exosomesmentioning

confidence: 99%

Exosomes in Nasopharyngeal Carcinoma

et al. 2018

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Exosomes are nanosized (30-100nm) membrane microvesicles secreted through a complex cellular process. Exosomes contain a variety of bioactive molecules, such as proteins, microRNAs(miRNAs or miRs) and long non-coding RNAs (lncRNAs), playing an important role in the cell-to-cell substance transportation and signal transduction. Nasopharyngeal carcinoma-related exosomes (NPC-Exo) have been identified in circulating blood and contribute to tumor cell proliferation, angiopoiesis, and immune tolerance through remodeling of tumor microenvironment (TME). Nasopharyngeal carcinoma-related exosomes may also induce epithelial-mesenchymal transition (EMT), thus promoting tumor metastasis and chemoradioresistance. Clinically, the exosomes may serve as novel biomarkers for diagnosis and targeted therapies of nasopharyngeal carcinoma. This review article updates the understanding of exosomes in nasopharyngeal carcinoma(NPC).

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“…Typically, 1 ml of culture medium will yield<1 µg of exosomal protein, while effective doses of exosomes range from 10 ug to100 ug. Certain techniques have been found to enhance exosome production, including the application of stressors such as low pH, hypoxia and anti-cancer drugs [109]. However, it is important to note that the effects of these techniques on exosome cargo are not fully understood, and caution must be taken in their evaluation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles Biology and its Emerging Role in Osteoarthritis and Related Arthritides

Attur¹,

Mignatti²,

Han³

et al. 2019

Rheumatology

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Extracellular vesicles (EV) are a heterogeneous group of membrane-enclosed structures that are shed from multiple cell types and can contain a variety of bioactive molecules including, nucleic acids, lipids, and proteins. They range in size from 50 nm to 5,000 nm and, based on their size, are sub-classified into exosomes, microvesicles and apoptotic bodies. While the content and formation of EV are diverse, research has yet to pinpoint their physiological and pathological roles. This review presents an overview of EV and a focus on exosomes, the most studied EV, and on the cellular processes that mediate their biogenesis. Understanding these mechanisms can shed light on their roles in physiology and disease, and indicate their potential therapeutic efficacy in clinical settings, especially in musculoskeletal diseases.

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Stressors alter intercellular communication and exosome profile of nasopharyngeal carcinoma cells

Cited by 41 publications

References 34 publications

Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress

Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress

Exosomes in Nasopharyngeal Carcinoma

Extracellular Vesicles Biology and its Emerging Role in Osteoarthritis and Related Arthritides

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