Stress responses of the oil-producing green microalga<i>Botryococcus braunii</i>Race B

Cornejo-Corona, Ivette; Thapa, Hem R.; Browne, Daniel R.; Devarenne, Timothy P.; Lozoya‐Gloria, Edmundo

doi:10.7717/peerj.2748

Cited by 12 publications

(6 citation statements)

References 91 publications

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“…Generation of reactive oxygen species (ROS) in the bacteria is hypothesized to be one of the reasons for antimicrobial activity of the GA + UV-A treatment against E. coli O157:H7. To evaluate this hypothesis, we used a fluorescent probe, CellROX ® Green Reagent for measuring oxidative stress in cells 25 – 28 . Figure 3a shows the results from CellROX ® fluorescence intensity measurement.…”

Section: Resultsmentioning

confidence: 99%

On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

Wang

Oliveira

Alborzi

et al. 2017

Sci Rep

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Possible mechanisms behind the enhanced antimicrobial activity of gallic acid (GA) and its ester propyl gallate (PG) in the presence of UV-A light against Escherichia coli O157:H7 were investigated. GA by itself is a mild antimicrobial and has a pro-oxidant ability. We found that the presence of UV-A light increases the uptake of GA by the bacteria. Once GA is internalized, the interaction between GA and UV-A induces intracellular ROS formation, leading to oxidative damage. Concurrently, GA + UV-A also inhibits the activity of superoxide dismutase (SOD), magnifying the imbalance of redox status of E. coli O157:H7. In addition to ROS induced damage, UV-A light and GA also cause injury to the cell membrane of E. coli O157:H7. UV-A exposed PG caused oxidative damage to the cell and significantly higher damage to the cell membrane than GA + UV-A treatment, explaining its higher effectiveness than GA + UV-A treatment. The findings presented here may be useful in developing new antimicrobial sanitation technologies for food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

Wang

Oliveira

Alborzi

et al. 2017

Sci Rep

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“…Since a high C/N ratio can be used for signatures of high-carbon compounds in the cell, the SXM visualization of the subcellular C/N ratio distribution can be applied to other physiological or cytological studies. Examples of such studies include oil production by microalgae (Suzuki et al, 2013;Khatri et al, 2014;Cornejo-Corona et al, 2016) and the CO 2 -concentrating processes in the algal pyrenoid (Freemen Rosenzweig et al, 2017) and the cyanobacterial carboxysome (Turmo et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria

et al. 2018

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Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium sp. PCC 7120, which can form heterocysts during nitrogen starvation. Statistical and spectroscopic analyses from SXM images around the K-absorption edge of nitrogen revealed a significant difference in the carbon-to-nitrogen (C/N) ratio between vegetative cells and heterocysts. Application of this analysis to soft x-ray images of sp. PCC 7120 revealed inhomogenous C/N ratios in the cells. Furthermore, soft x-ray tomography of sp. PCC 7120 revealed differing cellular C/N ratios, indicating different carbon and nitrogen distributions between vegetative cells and heterocysts in three dimensions.

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“…In addition, CellROX Green reagent (Invitrogen, Waltham, MA, United States) was used to detect the location of ROS by fluorescence microscopy following the method of Cornejo-Corona et al (2016) optimized for the present conditions. Briefly, cells irradiated on BG11 medium agar plates as above were resuspended in 100 μL of 0.1 M PBS.…”

Section: Methodsmentioning

confidence: 99%

Response of Endolithic Chroococcidiopsis Strains From the Polyextreme Atacama Desert to Light Radiation

et al. 2021

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Cyanobacteria exposed to high solar radiation make use of a series of defense mechanisms, including avoidance, antioxidant systems, and the production of photoprotective compounds such as scytonemin. Two cyanobacterial strains of the genus Chroococcidiopsis from the Atacama Desert – which has one of the highest solar radiation levels on Earth- were examined to determine their capacity to protect themselves from direct photosynthetically active (PAR) and ultraviolet radiation (UVR): the UAM813 strain, originally isolated from a cryptoendolithic microhabitat within halite (NaCl), and UAM816 strain originally isolated from a chasmoendolithic microhabitat within calcite (CaCO3). The oxidative stress induced by exposure to PAR or UVR + PAR was determined to observe their short-term response, as were the long-term scytonemin production, changes in metabolic activity and ultrastructural damage induced. Both strains showed oxidative stress to both types of light radiation. The UAM813 strain showed a lower acclimation capacity than the UAM816 strain, showing an ever-increasing accumulation of reactive oxygen species (ROS) and a smaller accumulation of scytonemin. This would appear to reflect differences in the adaptation strategies followed to meet the demands of their different microhabitats.

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Stress responses of the oil-producing green microalgaBotryococcus brauniiRace B

Cited by 12 publications

References 91 publications

On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria

Response of Endolithic Chroococcidiopsis Strains From the Polyextreme Atacama Desert to Light Radiation

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