Stress Responses of Bacterial Cells as Mechanism of Development of Antibiotic Tolerance (Review)

Ткаченко, А. Г.

doi:10.1134/s0003683818020114

Cited by 35 publications

(25 citation statements)

References 227 publications

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“…Adaptive molecular strategies ensure the ability of microorganisms to survive in environments significantly different from those, optimal for their growth, and have been the subject of active research over the past years [2][3][4][5][6][7][8]. Such adaptive strategy often launch the increasing synthesis (up to 150-200 thousand copies) of a ferritin-like protein Dps (DNA-binding protein of starved cells) [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…[9,15,[19][20][21]. The protective functions of Dps are carried out through condensation of DNA into "biocrystalline" or "in cellulo nanocrystalline" structures [2][3][4][5][6][7]19,[22][23][24] It is considered that the bacterial nucleoid represents an intermediate engineering solution between the protein-free DNA packaging in viruses and protein-defined DNA packaging in eukaryotes [25]. In diluted solutions, the diameter of a DNA double-helix is about 2 nm, while its length may reach up to several centimeters.…”

Section: Introductionmentioning

confidence: 99%

“…This corresponds to the heterogeneity of cells within a population. The heterogeneity of cells allows for a flexible response to environmental changes and helps to survive in stressful situations [5].…”

Section: Introductionmentioning

confidence: 99%

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Morphological peculiarities of DNA-protein complexes in dormantEscherichia colicells, subjected to prolonged starvation Condensation of DNA in dormant cells ofEscherichia coli

Krupyanskiǐ

Лойко

Danilova

et al. 2020

Preprint

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One of the adaptive strategies for the constantly changing conditions of the environment utilized in bacterial cells involves the condensation of DNA in complex with the DNA-binding protein, Dps. With the use of electron microscopy and electron tomography, we observed several morphologically different types of DNA condensation in dormant Escherichia coli cells, namely: nanocrystalline, liquid crystalline, and the folded nucleosome-like. We confirmed the presence of both Dps and DNA in all of the ordered structures using EDX analysis. The comparison of EDX spectra obtained for the three different ordered structures revealed that in nanocrystalline formation the majority of Dps protein is tightly bound to nucleoid DNA. We demonstrated that the population of the dormant cell is structurally heterogeneous, which allows cells to respond flexibly to environmental changes. It increases the ability of the whole bacterial population to survive under extreme stress conditions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological peculiarities of DNA-protein complexes in dormantEscherichia colicells, subjected to prolonged starvation Condensation of DNA in dormant cells ofEscherichia coli

Krupyanskiǐ

Лойко

Danilova

et al. 2020

Preprint

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“…The incidence of antibiotic resistant infections is rising globally, leading the world into a "post antibiotic era" and thus, the need to develop novel therapeutic interventions against bacterial pathogens is urgent. Emerging evidence suggests that targeting bacterial systems involved in stress response is a potential avenue for developing antimicrobials (Lee et al, 2009;Poole, 2014;Tkachenko, 2018). Therefore, identification and characterization of stress detection and detoxification mechanisms in pathogenic bacteria is of critical importance.…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial transcriptional changes in response to stress can be modulated by signal transduction systems known as two-component systems (TCSs). TCSs detect a wide range of signals and stressors including pH, temperature, nutrient, light, small molecules, envelope stress, osmotic pressure, and the redox state (Brunskill & Bayles, 1996;Fournier & Klier, 2004;Giraudo et al, 1997;Martin et al, 1999;Recsei et al, 1986;Tkachenko, 2018;Yarwood et al, 2001). TCSs enable cells to sense, respond, and adapt to changes in their environment and regulate a wide variety of processes including virulence, sporulation, antibiotic resistance, nutrient uptake, quorum sensing, and membrane integrity (Hoch, 2017;Mike et al, 2014;Stauff & Skaar, 2009;Tierney & Rather, 2019).…”

Section: Introductionmentioning

confidence: 99%

Directed evolution reveals the mechanism of HitRS signal transduction inBacillus anthracis

Skaar

Chu

et al. 2020

Preprint

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AbstractBacterial two component systems (TCSs) have been studied for decades; however, most work has focused on individual domains or proteins. Systematic characterization of an entire TCS could provide a mechanistic understanding of these important signal transduction systems. Here, genetic selections were employed to dissect the molecular basis of signal transduction by the HitRS system that has been implicated in detecting cell envelope stress in the pathogen Bacillus anthracis. Numerous point mutations were isolated within HitRS, 17 of which were in a 50-residue HAMP domain. Mutational analysis revealed the importance of hydrophobic interactions within the HAMP domain and highlighted its essentiality in TCS signaling. In addition, these data defined residues critical for activities intrinsic to HitRS, uncovered specific interactions among individual domains and between the two signaling proteins, and revealed that phosphotransfer is the rate-limiting step for signal transduction. This study establishes the use of unbiased genetic selections to study TCS signaling, provides a comprehensive mechanistic understanding of an entire TCS, and lays the foundation for development of novel antimicrobial therapeutics against this important infectious threat.

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Bacterial synthesized metal and metal salt nanoparticles in biomedical applications: An up and coming approach

Mohanta

Hashem

Abd_Allah

et al. 2020

Applied Organom Chemis

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In the present scenario, metal nanoparticles have elicited a great deal of interest in biomedical applications because of their unique properties and antimicrobial potentials. Over the past few years, the green nanotechnology has materialized as a momentous approach for the synthesis and fabrication of noble metal salt and metal nanoparticles. The green route synthesis exploits diverse reducing and stabilizing agents from bacterial resources for the successful synthesis of metal nanoparticles. This review mainly focuses on the biosynthesis of the most commonly studied metal and metal salt nanoparticles such as gold, silver, platinum, palladium, copper, cadmium, titanium oxide, zinc oxide, zinc sulphate, cadmium sulphide and many more. These noble nanoparticles can be exploited in pharmaceutical industry as antimicrobial and anti‐biofilm agents, targeted delivery of anticancer drugs, biosensors, etc.

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Stress Responses of Bacterial Cells as Mechanism of Development of Antibiotic Tolerance (Review)

Cited by 35 publications

References 227 publications

Morphological peculiarities of DNA-protein complexes in dormantEscherichia colicells, subjected to prolonged starvation Condensation of DNA in dormant cells ofEscherichia coli

Morphological peculiarities of DNA-protein complexes in dormantEscherichia colicells, subjected to prolonged starvation Condensation of DNA in dormant cells ofEscherichia coli

Directed evolution reveals the mechanism of HitRS signal transduction inBacillus anthracis

Bacterial synthesized metal and metal salt nanoparticles in biomedical applications: An up and coming approach

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