Stress Responses and Changes in Protein Metabolism in Carp Cyprinus carpio during Cadmium Exposure

Smet, Hyo Jung De; Blust, Ronny

doi:10.1006/eesa.2000.2011

Cited by 169 publications

(75 citation statements)

References 28 publications

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“…Presumably, this was induced by hypoxia resulting from impaired gas exchange at the gills, as has been reported in other fishes [29]. There were no clear changes in plasma Hb and protein, possibly because these secondary responses to environmental stress depend upon the exposure scenario and the acclimatization condition of fish [16,17,30]. Similar results have been reported on other fishes [3133].…”

Section: Hematological Responses To Cadmium Exposuresupporting

confidence: 79%

Exposure of silver carp (Hypophthalmichthys molitrix) to environmentally relevant levels of cadmium: hematology, muscle physiology, and implications for stock enhancement in the Xiangjiang River (Hunan, China)

Zhang

Zhuo

et al. 2013

Sci. China Life Sci.

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Cadmium is a non-essential metal with a wide distribution that has severe toxic effects on aquatic animals. Changes in hematology and muscle physiology were examined in silver carp (Hypophthalmichthys molitrix) exposed to environmentally relevant levels of cadmium (0.01 mg L 1 ) for 96 h. Cadmium exposure induced significant increases in the red blood cell count, and in the plasma concentrations of cortisol, glucose, and lactate. This suggests that the dose of cadmium was sufficient to cause stress, possibly associated with impaired gas exchange at the gills. There were no changes in hemoglobin concentration or plasma protein concentration. Significant decreases in muscle energy fuels (ATP and glycogen), and increases in muscle lactate persisted until the end of the exposure period, respectively. The changes in muscle lactate and protein in silver carp differed from those observed in response to exposure of fish to cadmium and heavy metals in other studies. The study highlights the importance of selecting unpolluted release sites with suitable water conditions for the survival of newly released individuals for stock enhancement of the Xiangjiang River. silver carp, hematology, muscle physiology, cadmium exposure, stock enhancement Citation:Zhang T, Zhang Y, Li D L, et al. Exposure of silver carp (Hypophthalmichthys molitrix) to environmentally relevant levels of cadmium: hematology, muscle physiology, and implications for stock enhancement in the Xiangjiang River

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Section: Hematological Responses To Cadmium Exposuresupporting

confidence: 79%

Exposure of silver carp (Hypophthalmichthys molitrix) to environmentally relevant levels of cadmium: hematology, muscle physiology, and implications for stock enhancement in the Xiangjiang River (Hunan, China)

Zhang

Zhuo

et al. 2013

Sci. China Life Sci.

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“…Tissues found to be positive for hsp70 ( Figure 3) and hsp70-eGFP (Figure 4) in our experiments are considered target tissues or accumulators of Cd in fish (30)(31)(32), and histopathologic lesions in skin, digestive tract, olfactory epithelium, kidney, and gills have been reported after a 96-hr acute exposure to 12 ppm CdCl 2 (~66 µM) (33). This dose is within our exposure range, but expression of hsp70 and hsp70-eGFP was detected in developing tissues after a much shorter period of exposure (3 vs. 96 hr).…”

Section: Discussionmentioning

confidence: 81%

“…Stromberg et al (33) found that adult tissues directly exposed to Cd in water (gills, olfactory tissue, and skin) exhibited more severe lesions than did more visceral tissues. Gills are also the osmoregulatory organ in fish and therefore are the principal route of absorption for waterborne Cd (31,35), although it is not known whether the developing larval gill plays a similar role. The liver and pronephros expressed endogenous hsp70 and eGFP only at the highest 125-µM dose, indicating a lower degree of sensitivity to Cd relative to the skin, gill, and olfactory tissues.…”

Section: Discussionmentioning

confidence: 99%

Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line.

Blechinger

Warren

Kuwada

et al. 2002

Environ Health Perspect

146

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The toxic effects of cadmium and other heavy metals have been well established, and many of these and other environmental pollutants are known to be embryotoxic or teratogenic. However, it has proven difficult to identify individual cells that respond to toxicants among the wide range of cell populations in an intact animal, particularly during early development when cells are continually changing their molecular and physiologic characteristics as they differentiate. Here we report the establishment of an in vivo system that uses hsp70 gene activation as a measure of cadmium toxicity in living early larvae of transgenic zebrafish carrying a stably integrated hsp70-enhanced green fluorescent protein (eGFP) reporter gene. We demonstrate that eGFP expression in this strain of fish acts as an accurate and reproducible indicator of cell-specific induction of hsp70 gene expression. Furthermore, the transgene responds in a dose-dependent manner at concentrations similar to those observed for morphologic indicators of early-life-stage toxicity and is sensitive enough to detect cadmium at doses below the median combined adverse effect concentration and the median lethal concentration. The stable nature of this transgenic line should allow for extremely rapid and reproducible toxicologic profiling of embryos and larvae throughout development.

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“…The entry/accumulation of toxins in the liver may increase protein and carbohydrate metabolism to account for the energy crisis or need for detoxification during stress, leading to increased ALT and AST activities (De Smet and Blust, 2001;Malarvizhi et al, 2012). The results of the current and previous studies thus suggest that ALT and AST activities in zebrafish liver may be induced by aphantoxins/PSPs in response to the increased metabolism associated with hepatic dysfunction and liver structural damage (Loteste et al, 2013).…”

Section: Alt and Ast Activitiesmentioning

confidence: 68%

Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins

Zhang

et al. 2014

Aquatic Toxicology

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Stress Responses and Changes in Protein Metabolism in Carp Cyprinus carpio during Cadmium Exposure

Cited by 169 publications

References 28 publications

Exposure of silver carp (Hypophthalmichthys molitrix) to environmentally relevant levels of cadmium: hematology, muscle physiology, and implications for stock enhancement in the Xiangjiang River (Hunan, China)

Exposure of silver carp (Hypophthalmichthys molitrix) to environmentally relevant levels of cadmium: hematology, muscle physiology, and implications for stock enhancement in the Xiangjiang River (Hunan, China)

Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line.

Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins

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