Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF

Abstract

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.

Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver

“…Camele et al [ 28 ] described that the quorum sensing mechanism is deteriorated by the influence of EOs and that disrupting the quorum sensing mechanism can help to prevent biofilm formation. Moreover, the differences in protein production of bacteria stressed with EOs was evaluated with MALDI-TOF MS by Božik et al [ 29 ] demonstrating that EOs affect the production of ribosomal proteins and stress-related, membrane-related, and biofilm-related proteins.…”

Data on low-molecular weight proteins of Escherichia coli treated by essential oils components, tetracycline, chlorine and peroxide by MALDI-TOF MS

“…The signal intensities were normalized to the same median of intensity ratios. For a protein identification and comprehensive discussion and of the full dataset, please see the research article that accompanies this data article (Božik et al, 2018) [1] .…”

“… In this dataset we provide MALDI-TOF MS spectra of Escherichia coli. The data presented in this article are related to the article entitled “Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF” (Božik et al, 2018) [1] . Essential oils and their components are known for their antibacterial effect.…”

Synthesis and Matrix Properties of α-Cyano-5-phenyl-2,4-pentadienic Acid (CPPA) for Intact Proteins Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

“…Here, since the detected proteins exhibit relatively low MWs (i.e., <10 kDa), CClCA produced a good spectrum, confirming its efficacy in promoting the ionization of peptides or small proteins compared to CHCA [ 32 ]. Although it is not possible to distinguish a protein from its intact molecular weight, a tentative assignment of peaks detected in Figure 7 A was pursued by combining literature data [ 58 , 63 ] with database mining. Specifically, the experimental m / z values were used as search parameters and were matched with sequence-derived theoretical MW values in the UniProtKB/Swiss-Prot database [ 58 , 64 ].…”