Stress Protection of Transgenic Tobacco by Production of the Osmolyte Mannitol

Tarczynski, Mitchell C.; Jensen, Richard G.; Bohnert, Hans J.

doi:10.1126/science.259.5094.508

Cited by 600 publications

(287 citation statements)

References 15 publications

(12 reference statements)

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“…Recently, results from metabolic engineering experiments have confirmed the functional roles for compatible osmolytes, such as mannital, proline, and betaine, in salt adaptation of high plants [1], [2], [3]. These compounds are presumed to function both in osmoprotection and osmotic adjustment.…”

Section: Introductionmentioning

confidence: 93%

HAL1 mediate salt adaptation in Arabidopsis thaliana

Yang

Zhang

et al. 2001

Cell Res

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The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. Several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K + and Na + showed that transgenic lines were able to retain less Na + than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.

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Section: Introductionmentioning

confidence: 93%

HAL1 mediate salt adaptation in Arabidopsis thaliana

Yang

Zhang

et al. 2001

Cell Res

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“…The actual role of carbohydrates should now be determined by compartmentation studies and analytical determinations on subcellular fractions. Finally the protective effect of sugars should be confirmed by experiments aiming at changing the Eucalyptus cell carbohydrate pattern through genetic engineering, as performed in the case of mannitol for salt stress protection (Tarczynski et al, 1993). Transformation procedures for Eucalyptus are now available for this purpose (Teulikres et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Leborgne

Teulières

Travert

et al. 1995

European Journal of Biochemistry

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The comparison of soluble sugar content in various cell lines of Eucalyptus gunnii exhibiting different freezing resistances revealed that the most resistant cell line contained the highest soluble sugar content. It was possible to increase the freezing resistance of the sensitive cell line by progressive exposure to low temperatures (acclimation). During the early stage of cold acclimation, an increase of soluble sugar concentration was observed in the cells confirming the correlation between freezing resistance and soluble carbohydrate content in this species. In addition, feeding experiments on the sensitive cell line were performed to introduce specific sugars into the cells. Both electropulsation and long‐term incubation in the presence of fructose and raffinose led to an increase in the tolerance of the cells during a freezing programme. Using radioactive fructose, the uptake of the sugar into cells and protoplasts was checked. In the light of these results, hypotheses are presented concerning the possible role of intracellular sugars in cryoprotection.

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“…Specialized metabolites also are the source of many of our plant‐based medicines and therefore have value to human health and well‐being (Briskin, 2000). Much attention has therefore been placed on environmental and geographic factors influencing specialized metabolite production in plants, particularly in crop species and in model plant systems (Agrawal, Conner, Johnson, & Wallsgrove, 2002; Asai, Matsukawa, & Kajiyamal, 2016; Carrari et al., 2006; Dan et al., 2016; Hirai et al., 2004; Lasky et al., 2012; Tarczynski, Jensen, & Bohnert, 1993; Riedelsheimer et al., 2012). However, the importance of natural variation in the environment in explaining metabolite variation within plant species remains little understood (Maldonado et al., 2017; Moore, Andrew, Külheim, & Foley, 2014).…”

Section: Introductionmentioning

confidence: 99%

Untargeted metabolic profiling reveals geography as the strongest predictor of metabolic phenotypes of a cosmopolitan weed

Ahlstrand

Reghev

Markussen

et al. 2018

Ecology and Evolution

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Plants produce a multitude of metabolites that contribute to their fitness and survival and play a role in local adaptation to environmental conditions. The effects of environmental variation are particularly well studied within the genus Plantago; however, previous studies have largely focused on targeting specific metabolites. Studies exploring metabolome‐wide changes are lacking, and the effects of natural environmental variation and herbivory on the metabolomes of plants growing in situ remain unknown. An untargeted metabolomic approach using ultra‐high‐performance liquid chromatography–mass spectrometry, coupled with variation partitioning, general linear mixed modeling, and network analysis was used to detect differences in metabolic phenotypes of Plantago major in fifteen natural populations across Denmark. Geographic region, distance, habitat type, phenological stage, soil parameters, light levels, and leaf area were investigated for their relative contributions to explaining differences in foliar metabolomes. Herbivory effects were further investigated by comparing metabolomes from damaged and undamaged leaves from each plant. Geographic region explained the greatest number of significant metabolic differences. Soil pH had the second largest effect, followed by habitat and leaf area, while phenological stage had no effect. No evidence of the induction of metabolic features was found between leaves damaged by herbivores compared to undamaged leaves on the same plant. Differences in metabolic phenotypes explained by geographic factors are attributed to genotypic variation and/or unmeasured environmental factors that differ at the regional level in Denmark. A small number of specialized features in the metabolome may be involved in facilitating the success of a widespread species such as Plantago major into such wide range of environmental conditions, although overall resilience in the metabolome was found in response to environmental parameters tested. Untargeted metabolomic approaches have great potential to improve our understanding of how specialized plant metabolites respond to environmental change and assist in adaptation to local conditions.

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Stress Protection of Transgenic Tobacco by Production of the Osmolyte Mannitol

Cited by 600 publications

References 15 publications

HAL1 mediate salt adaptation in Arabidopsis thaliana

HAL1 mediate salt adaptation in Arabidopsis thaliana

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Untargeted metabolic profiling reveals geography as the strongest predictor of metabolic phenotypes of a cosmopolitan weed

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