Stress-induced somatic embryogenesis on seedlings of Azadirachta indica A. Juss. by thidiazuron and its inhibition by ethylene modulators

Nautiyal, Aparna; Ramlal, Ayyagari; Agnihotri, Ashish; Rashid, Abdur

doi:10.1007/s11240-023-02473-y

Cited by 3 publications

(5 citation statements)

References 49 publications

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“…In addition, Thidiazuron (TDZ; N-phenyl-N′-1,2,3-thiadiazol-5-ylurea) was recognized as a potent regulator in vitro as early as 1990, with cytokinin-likes responses [ 130 ]. In addition to its cytokinin-like effects, TDZ can also be considered as a stress factor [ 131 ]. Currently, TDZ is used successfully in many plant species (for a review, see [ 132 , 133 ]).…”

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

“…Ethylene should get special attention in PTC since plant growth is relatively small in isolated volumes with lower air exchange rates than in natural conditions, a system with a high risk of ethylene accumulation. During the induction step, high ethylene concentration has a positive effect on somatic embryo induction [ 131 ] by promoting auxin biosynthesis and the inhibition of transport [ 153 , 154 ]. Moreover, ethylene also influences mineral nutrition through auxin signaling [ 155 , 156 ].…”

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

“…In PTC, stress-inducing factors are key for morphogenesis induction through creating the organizer cell niche by the initial accumulation of high auxin contents, the induction of histone hyperacetylation, and auxin biosynthesis induction in specific cell types [ 164 ]. In this case, with a few exceptions (i.e., heat shock on microspore embryogenesis), this effect of the stress-induced agents can be achieved only in combination with exogenous 2,4,-D [ 165 ] or cytokinin (TDZ) [ 131 ]. Stress factors in general play a key role in embryo induction during microspore embryogenesis through changes in epigenetics and, therefore, auxin homeostasis [ 166 ].…”

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

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Plant Growth Regulation in Cell and Tissue Culture In Vitro

Pasternak,

Steinmacher

2024

Plants

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Precise knowledge of all aspects controlling plant tissue culture and in vitro plant regeneration is crucial for plant biotechnologists and their correlated industry, as there is increasing demand for this scientific knowledge, resulting in more productive and resilient plants in the field. However, the development and application of cell and tissue culture techniques are usually based on empirical studies, although some data-driven models are available. Overall, the success of plant tissue culture is dependent on several factors such as available nutrients, endogenous auxin synthesis, organic compounds, and environment conditions. In this review, the most important aspects are described one by one, with some practical recommendations based on basic research in plant physiology and sharing our practical experience from over 20 years of research in this field. The main aim is to help new plant biotechnologists and increase the impact of the plant tissue culture industry worldwide.

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Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

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Plant Growth Regulation in Cell and Tissue Culture In Vitro

Pasternak,

Steinmacher

2024

Plants

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“…Aseptic seedling tissues (cotyledons, petioles, tubers, and roots) have also been used as explants to initiate SE culture in many medicinal and endemic geophytes (e.g., Crocus, Iris, Hypoxis, Colchicum, etc.) [135,143,163,164,[175][176][177][178]. Some embryo-like structures from P. maritimum bulb explants are shown in Figure 7A-C.…”

Section: Stage 2: Multiplicationmentioning

confidence: 99%

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

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This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and bulb growth. Bioreactor systems for healthy plant regeneration, rooting methods, acclimatization strategies, and considerations for ex vitro survival are elucidated. The review also delves into somaclonal variation dynamics and acknowledges the burgeoning field of gene editing, particularly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) studies, as a promising avenue for enhancing valuable compound content in geophytes. In addition to addressing challenges in flower bulb micropropagation, this review briefly highlights emerging opportunities, including the potential integration of artificial intelligence (AI) to optimize culture conditions, predict growth parameters, and enhance efficiency in bulb production. The conclusion emphasizes the necessity of a multifaceted approach integrating biochemistry, physiology, and molecular biology to address existing challenges and improve tissue culture protocols for diverse geophyte species. This review article also intends to highlight how tissue culture techniques could contribute to the development and valorization of flower bulbs in today’s scenario of the ornamental industry.

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“…Crocus, Iris, Hypoxis,Colchicum etc.) [123,131,153,154,[165][166][167][168]. Some embryo-like structures from P maritimum bulb explants are shown in Figure 7A, 7B, and 7C.…”

Section: Stage 2: Multiplicationmentioning

confidence: 99%

A Review on Geophytes Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Preprint

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Stress-induced somatic embryogenesis on seedlings of Azadirachta indica A. Juss. by thidiazuron and its inhibition by ethylene modulators

Cited by 3 publications

References 49 publications

Plant Growth Regulation in Cell and Tissue Culture In Vitro

Plant Growth Regulation in Cell and Tissue Culture In Vitro

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

A Review on Geophytes Micropropagation: Challenges and Opportunities

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