Stress induced phosphoprotein 1 overexpression controls proliferation, migration and invasion and is associated with poor survival in oral squamous cell carcinoma

Abstract

ObjectiveAlthough there have been remarkable achievements in the molecular landscape of oral squamous cell carcinoma (OSCC) in recent years, bringing advances in the understanding of its pathogenesis, development and progression, little has been applied in the prognosis and choosing the optimal treatment. In this study, we explored the influence of the stress induced phosphoprotein 1 (STIP1), which is frequently reported to be highly expressed in many cancers, in OSCCs.MethodsSTIP1 expression was assessed in the TCGA database and in two independent cohorts by immunohistochemistry. Knockdown strategy was applied in OSCC cell lines to determine the impact of STIP1 on viability, proliferation, migration and invasion. The zebrafish model was applied for studying tumor formation and metastasis in vivo. The association of STIP1 and miR-218-5p was explored by bioinformatics and mimics transfection.ResultsSTIP1 was highly expressed in OSCCs and significantly associated with shortened survival and higher risk of recurrence. STIP1 down-regulation decreased proliferation, migration and invasion of tumor cells, and reduced the number of metastases in the Zebrafish model. STIP1 and miR-218-5p were inversely expressed, and the transfection of miR-218-5p mimics into OSCC cells decreased STIP1 levels as well as proliferation, migration and invasion.ConclusionOur findings show that STIP1 overexpression, which is inversely associated with miR-218-5p levels, contributes to OSCC aggressiveness by controlling proliferation, migration and invasion and is a determinant of poor prognosis.

High-Multiplex Aptamer-Based Serum Proteomics to Identify Candidate Serum Biomarkers of Oral Squamous Cell Carcinoma

“…Among these 12 proteins, several were overexpressed proteins, with links to OSCC or cancer such as interstitial collagenase (MMP1), stress-induced-phosphoprotein 1 (STIP1), glia-derived nexin (SERPINE2), membrane frizzled-related protein (MFRP) and MAP kinase-activated protein kinase 3 (MAPKAPK3). For example, STIP1 is overexpressed in OSCC and its expression directly correlates with poor survival and an increased risk of recurrence [ 57 ]. SERPINE2 has also been reported to be highly expressed in OSCC and to induce angiogenesis [ 58 ].…”

Overexpression of heat‐shock protein 47 impacts survival of patients with oral squamous cell carcinoma

“…The 24‐well plates with transwell inserts of polycarbonate with 6.5 mm diameter and 8 μm pore were used to evaluate the migration and invasion of the cells, as previously reported by us 21 . In the invasion assays, the membrane of inserts was covered with myogel.…”

Aptamers as Potential Therapeutic Tools for Ovarian Cancer: Advancements and Challenges

“…This complex plays a critical role in the folding and maturation of transcription factors, hormone receptors, and protein kinases. Hsps are implicated in the initiation, facilitation, and progression of malignant conditions [120][121][122]. Reports have indicated the overexpression of STIP1 in ovarian, breast, renal, gastric, and colorectal cancers and oral squamous cell carcinoma [121,122].…”

“…Hsps are implicated in the initiation, facilitation, and progression of malignant conditions [120][121][122]. Reports have indicated the overexpression of STIP1 in ovarian, breast, renal, gastric, and colorectal cancers and oral squamous cell carcinoma [121,122]. In human OC, STIP1 has been identified as a released biomarker.…”