Stress elicits trans-synaptic activation of adrenal neuropeptide Y gene expression

Hiremagalur, Bhargava; Květňanský, Richard; Nankova, Bistra B.; Fleischer, Joseph; Geertman, Robert T.; Fukuhara, Kazunobu; Viskupic, E; Sabban, Esther L.

doi:10.1016/0169-328x(94)90194-5

Cited by 41 publications

(33 citation statements)

References 25 publications

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“…The biosynthesis of secreted peptides, such as proenkephalin A (7) and neuropeptide Y (8), is likewise activated by nicotinic stimulation. Chromogranin A is the quantitatively major protein stored and released with catecholamines (2).…”

Section: Introductionmentioning

confidence: 99%

Stimulus coupling to transcription versus secretion in pheochromocytoma cells. Convergent and divergent signal transduction pathways and the crucial roles for route of cytosolic calcium entry and protein kinase C.

Tang

Wu²,

Mahata³

et al. 1997

J. Clin. Invest.

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How do chromaffin cell secretory stimuli program resynthesis of secreted peptides and amines? We previously showed that the physiologic nicotinic cholinergic signal for secretion also activates the biosynthesis of chromogranin A, the major protein released with catecholamines. Here, we examine signal transduction pathways whereby secretory stimuli influence exocytotic secretion versus chromogranin A transcription. Both secretion and transcription depended on initial nicotinic-triggered sodium entry into the cytosol, followed by calcium entry through L -type voltage-gated channels. When calcium entered through L -type channels, activation of secretion paralleled activation of transcription ( r ϭ 0.897, P ϭ 0.002). Calcium entry from intracellular stores or through calcium ionophore channels activated secretion, though not transcription. Nicotinic-stimulated transcription depended upon protein kinase C activation; nicotine caused translocation of protein kinase C to the cell membrane fraction, and inhibition of protein kinase C blocked activation of transcription, while activation of protein kinase C mimicked nicotine effects. Transcriptional responses to both nicotine and protein kinase C mapped principally onto the chromogranin A promoter's cAMP response element (TGACGTAA; CRE box). KCREB, a dominant negative mutant of the CREbinding protein CREB, blunted activation of chromogranin A transcription by nicotine, phorbol ester, or membrane depolarization. We conclude that activation of chromogranin A transcription by secretory stimulation in chromaffin cells is highly dependent upon precise route of calcium entry into the cytosol; transcription occurred after entry of calcium through L -type channels on the cell surface, and was mediated by protein kinase C activation. The trans -acting factor CREB ultimately relays the secretory signal to the chromogranin A promoter's CRE box in cis. ( J. Clin. Invest.

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Section: Introductionmentioning

confidence: 99%

Stimulus coupling to transcription versus secretion in pheochromocytoma cells. Convergent and divergent signal transduction pathways and the crucial roles for route of cytosolic calcium entry and protein kinase C.

Tang

Wu²,

Mahata³

et al. 1997

J. Clin. Invest.

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“…A large-scale stress-antileptic physiological reaction refers to a complicated neural endocrine, and the interaction between the immune system to preserve internal homeostasis and to respond to life-threatening unexpected events (43). The first sign of the stress response is the activation of the sympathetic nervous system.…”

Section: Discussionmentioning

confidence: 99%

Effects of lycium barbarum polysaccharides on neuropeptide Y and heat-shock protein 70 expression in rats exposed to heat

et al. 2014

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Abstract. The purpose of the present study was to investigate the effects of high ambient temperature on the neuropeptide Y (NPY) mRNA level in the hypothalamus, the plasma concentration of corticotropin-releasing hormone (CRH), cortisol (Cor), heat-shock protein 70 (HSP70) and epinephrine (EPI), and the intervention of lycium barbarum polysaccharides (LBPs) in rats. Compared to the control (CN) group, the plasma levels of CRH, Cor, HSP70 and EPI were markedly increased, and the level of NPY mRNA was downregulated in the high ambient temperature (HT) group. By contrast, rats in the HT + LBP (HTL) group had: i) a significantly enhanced expression of HSP70 compared to the HT and CN groups; ii) clearly increased plasma levels of CRH, Cor and EPI compared to the CN group; and iii) a markedly upregulated expression of NPY mRNA compared to the HT group. Thus, the results showed that high-temperature environments may damage the body, and LBPs have a potentially protective function by increasing the expression of HSP70 and NPY.

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“…Alternative or additional explanation takes into consideration the influence of neuropeptide Y (NPY) on the adrenal blood vessels because this peptide causes vasoconstriction [21]. Cold stress cause increases in rat adrenal NPY mRNA [22]. The vasoconstrictor NPY coexists with noradrenaline in perivascular sympathetic nerves and its functional roles include potent vasoconstriction in the hyperadrenergic state [23], such as exposure to stressogenic stimuli.…”

Section: Discussionmentioning

confidence: 99%

Effect of acute heat stress on rat adrenal cortex — a morphological and ultrastructural study

Petrovic-Kosanovic¹,

Veličković²,

Koko³

et al. 2012

Open Life Sciences

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The stereological structure of rat adrenal gland was analysed by light and electron microscopy after an acute (60 min) exposure to high ambient temperature (38°C). Under these conditions a significant increase in plasma corticotrophin (ACTH), serum corticosterone and aldosterone levels were observed. Histological and stereological investigation at light microscopy showed significant decrease in volume density of capsule and zona glomerulosa, increase in volume of fasciculata cells, and decrease of numerical density of zona fasciculata cells and mean diameter of blood vessels. At the ultrastructural level, volume density of nuclei and mitochondria of zona glomerulosa cells were significantly increased and that of lipid droplets decreased. Volume density of mitochondria of fasciculata cells was significantly increased, while number of lipid droplets per µm2 of cell was reduced. In the cells of zona reticularis significant increase in the number of lipid droplets was found. The response of zona glomerulosa may be interpreted as immediate reaction to dehydration, while alterations detected in zona fasciculata, which were less extensive, were related to purely stressogenic effects of high ambiental temperature.

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Stress elicits trans-synaptic activation of adrenal neuropeptide Y gene expression

Cited by 41 publications

References 25 publications

Stimulus coupling to transcription versus secretion in pheochromocytoma cells. Convergent and divergent signal transduction pathways and the crucial roles for route of cytosolic calcium entry and protein kinase C.

Stimulus coupling to transcription versus secretion in pheochromocytoma cells. Convergent and divergent signal transduction pathways and the crucial roles for route of cytosolic calcium entry and protein kinase C.

Effects of lycium barbarum polysaccharides on neuropeptide Y and heat-shock protein 70 expression in rats exposed to heat

Effect of acute heat stress on rat adrenal cortex — a morphological and ultrastructural study

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